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Molecules 2015, 20(2), 3431-3462; doi:10.3390/molecules20023431

Analysis of Phenolic and Cyclic Compounds in Plants Using Derivatization Techniques in Combination with GC-MS-Based Metabolite Profiling

Department of Biology, Norwegian University of Science and Technology, Trondheim 7491, Norway
Academic Editor: Derek J. McPhee
Received: 18 December 2014 / Revised: 6 January 2015 / Accepted: 10 February 2015 / Published: 17 February 2015
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [4126 KB, uploaded 17 February 2015]   |  

Abstract

Metabolite profiling has been established as a modern technology platform for the description of complex chemical matrices and compound identification in biological samples. Gas chromatography coupled with mass spectrometry (GC-MS) in particular is a fast and accurate method widely applied in diagnostics, functional genomics and for screening purposes. Following solvent extraction and derivatization, hundreds of metabolites from different chemical groups can be characterized in one analytical run. Besides sugars, acids, and polyols, diverse phenolic and other cyclic metabolites can be efficiently detected by metabolite profiling. The review describes own results from plant research to exemplify the applicability of GC-MS profiling and concurrent detection and identification of phenolics and other cyclic structures. View Full-Text
Keywords: derivatization; food chemistry; gas chromatography; mass spectrometry; phenols; phenolic acids derivatization; food chemistry; gas chromatography; mass spectrometry; phenols; phenolic acids
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Rohloff, J. Analysis of Phenolic and Cyclic Compounds in Plants Using Derivatization Techniques in Combination with GC-MS-Based Metabolite Profiling. Molecules 2015, 20, 3431-3462.

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