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Molecules 2015, 20(2), 2576-2590; doi:10.3390/molecules20022576

Anti-Tuberculous Activity of Treponemycin Produced by a Streptomyces Strain MS-6-6 Isolated from Saudi Arabia

1
Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, P.O. Box 80260, Jeddah 21589, Saudi Arabia
2
Department of Microbiology and Immunology, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt
3
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt
4
Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, P.O. Box 80260, Jeddah 21589, Saudi Arabia
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 21 December 2014 / Accepted: 27 January 2015 / Published: 2 February 2015
(This article belongs to the Section Natural Products)
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Abstract

A Streptomyces strain MS-6-6 with promising anti-tuberculous activity was isolated from soil samples in Saudi Arabia. The nucleotide sequence of its 16S rRNA gene (1426 bp) evidenced a 100% similarity to Streptomyces mutabilis. Through an anti-tuberculous activity-guided approach, a polyketide macrolide was isolated and identified as treponemycin (TP). The structure of the isolated compound was determined by comprehensive analyses of its 1D and 2D NMR as well as HRESI-MS. In addition to the promising anti-tuberculous activity (MIC = 13.3 µg/mL), TP showed broad spectrum of activity against the Gram positive, Gram negative strains, and Candida albicans. Improvement of TP productivity (150%) was achieved through modification in liquid starch nitrate medium by replacing KNO3 with corn steep liquor and yeast extract or tryptone, and removing CaCO3 and K2HPO4. The follow up of TP percentage as well as its metabolites profile for each media was assessed by LC/DAD/MS. View Full-Text
Keywords: Streptomyces mutabilis; treponemycin; anti-tuberculous activity Streptomyces mutabilis; treponemycin; anti-tuberculous activity
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MDPI and ACS Style

Yassien, M.A.; Abdallah, H.M.; El-Halawany, A.M.; Jiman-Fatani, A.A.M. Anti-Tuberculous Activity of Treponemycin Produced by a Streptomyces Strain MS-6-6 Isolated from Saudi Arabia. Molecules 2015, 20, 2576-2590.

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