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Correction published on 12 July 2016, see Molecules 2016, 21(7), 907.

Open AccessArticle
Molecules 2015, 20(12), 22674-22690; doi:10.3390/molecules201219868

Short-Term and Sub-Chronic Dietary Exposure to Aspalathin-Enriched Green Rooibos (Aspalathus linearis) Extract Affects Rat Liver Function and Antioxidant Status

1
Department of Food Science, Stellenbosch University, Private Bag X1, Matieland (Stellenbosch) 7602, South Africa
2
Post-Harvest and Wine Technology Division, Agricultural Research Council (ARC), Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa
3
Institute of Biomedical and Microbial Biotechnology, Cape Peninsula University of Technology, P. O. Box 1906, Bellville 7535, South Africa
4
Department of Biochemistry, Stellenbosch University, Private Bag X1, Matieland (Stellenbosch) 7602, South Africa
Current address: Food and Allergy Consulting and Testing Services, 18 Uplands Road, Cape Town 7435, South Africa
*
Author to whom correspondence should be addressed.
Academic Editor: David D. Kitts
Received: 9 November 2015 / Revised: 9 December 2015 / Accepted: 11 December 2015 / Published: 18 December 2015
(This article belongs to the Special Issue Antioxidants—A Risk-Benefit Analysis for Health)
View Full-Text   |   Download PDF [1075 KB, uploaded 12 July 2016]   |  

Abstract

An aspalathin-enriched green rooibos (Aspalathus linearis) extract (GRE) was fed to male Fischer rats in two independent studies for 28 and 90 days. The average dietary total polyphenol (TP) intake was 756 and 627 mg Gallic acid equivalents (GAE)/kg body weight (bw)/day over 28 and 90 days, respectively, equaling human equivalent doses (HEDs) of 123 and 102 GAE mg/kg bw/day. Aspalathin intake of 295 mg/kg bw/day represents a HED of 48 mg/kg bw/day (90 day study). Consumption of GRE increased feed intake significantly (p < 0.05) compared to the control after 90 days, but no effect on body and organ weight parameters was observed. GRE significantly (p < 0.05) reduced serum total cholesterol and iron levels, whilst significantly (p < 0.05) increasing alkaline phosphatase enzyme activity after 90 days. Endogenous antioxidant enzyme activity in the liver, i.e., catalase and superoxide dismutase activity, was not adversely affected. Glutathione reductase activity significantly (p < 0.05) increased after 28 days, while glutathione (GSH) content was decreased after 90 days, suggesting an altered glutathione redox cycle. Quantitative Real Time polymerase chain reaction (PCR) analysis showed altered expression of certain antioxidant defense and oxidative stress related genes, indicative, among others, of an underlying oxidative stress related to changes in the GSH redox pathway and possible biliary dysfunction. View Full-Text
Keywords: herbal tea; biochemical parameters; serum iron; endogenous antioxidant enzymes; gene expression herbal tea; biochemical parameters; serum iron; endogenous antioxidant enzymes; gene expression
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MDPI and ACS Style

van der Merwe, J.D.; de Beer, D.; Joubert, E.; Gelderblom, W.C.A. Short-Term and Sub-Chronic Dietary Exposure to Aspalathin-Enriched Green Rooibos (Aspalathus linearis) Extract Affects Rat Liver Function and Antioxidant Status. Molecules 2015, 20, 22674-22690.

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