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Molecules 2015, 20(12), 21298-21312; doi:10.3390/molecules201219766

Oligonucleotide Functionalised Microbeads: Indispensable Tools for High-Throughput Aptamer Selection

School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editors: Alain O. A. Miller and Jean Jacques Vanden Eynde
Received: 22 September 2015 / Revised: 21 October 2015 / Accepted: 12 November 2015 / Published: 1 December 2015
(This article belongs to the Special Issue Aptamers: Past, Present, and Future)
View Full-Text   |   Download PDF [1479 KB, uploaded 1 December 2015]   |  

Abstract

The functionalisation of microbeads with oligonucleotides has become an indispensable technique for high-throughput aptamer selection in SELEX protocols. In addition to simplifying the separation of binding and non-binding aptamer candidates, microbeads have facilitated the integration of other technologies such as emulsion PCR (ePCR) and Fluorescence Activated Cell Sorting (FACS) to high-throughput selection techniques. Within these systems, monoclonal aptamer microbeads can be individually generated and assayed to assess aptamer candidate fitness thereby helping eliminate stochastic effects which are common to classical SELEX techniques. Such techniques have given rise to aptamers with 1000 times greater binding affinities when compared to traditional SELEX. Another emerging technique is Fluorescence Activated Droplet Sorting (FADS) whereby selection does not rely on binding capture allowing evolution of a greater diversity of aptamer properties such as fluorescence or enzymatic activity. Within this review we explore examples and applications of oligonucleotide functionalised microbeads in aptamer selection and reflect upon new opportunities arising for aptamer science. View Full-Text
Keywords: aptamer; SELEX; microbead; monoclonal bead; ePCR; FACS aptamer; SELEX; microbead; monoclonal bead; ePCR; FACS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Fraser, L.A.; Kinghorn, A.B.; Tang, M.S.L.; Cheung, Y.-W.; Lim, B.; Liang, S.; Dirkzwager, R.M.; Tanner, J.A. Oligonucleotide Functionalised Microbeads: Indispensable Tools for High-Throughput Aptamer Selection. Molecules 2015, 20, 21298-21312.

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