Analysis of Chemical Constituents in Wuzi-Yanzong-Wan by UPLC-ESI-LTQ-Orbitrap-MS

Abstract

Wuzi-Yanzong-Wan (WZYZW), a classical traditional Chinese medicine (TCM) prescription containing Fructus Lych, Semen Cuscutae (fried), Fructus Rubi, Fructus Schisandrae chinensis (steamed) and Semen Plantaginis (fried with salt), is widely used to treat impotence, sterility, spermatorrhea, premature ejaculation, lumbago and post-micturation dribble. However, the chemical profile of WZYZW has not been established yet. In this work, a rapid and sensitive method for systematically screening and identifying the chemical constituents of WZYZW in both positive and negative ion modes using Ultra-Performance LC coupled with ESI-linear ion trap-Orbitrap tandem mass spectrometry (UPLC-ESI-LTQ-Orbitrap-MS) has been developed. Based on the chromatographic and spectrometric data, and referring to the literature, we could tentatively identify 106 compounds, including organic acids, flavonoids, phenylpropanoids, alkaloids and terpenoids. Fourteen ingredients from Fructus Lych were identified, while 10 ingredients were from Semen Cuscutae (fried), 33 ingredients were from Fructus Rubi, 37 ingredients were from Fructus Schisandrae chinensis (steamed), and 20 ingredients were from Semen Plantaginis (fried with salt). The results may provide essential data for further quality control, pharmacological research and clinical evaluation of WZYZW. Furthermore, this study indicates the developed approach based on UPLC-ESI-LTQ-Orbitrap-MS is suitable for characterizing the chemical profiles of TCM prescriptions. This is the first report to provide a comprehensive analysis of the chemical constituents of WZYZW.

1. Introduction

Chinese herbal prescriptions, the basic form of clinical application of TCM for thousands of years, have been proven by clinical practice to play a positive role in human health. The multi-chemical constituents of Chinese herbal prescriptions are the key to achieving their prevention and treatment effects, so comprehensive analysis of their chemical constituents is the premise and foundation to promote development and innovation in TCM [1,2,3]. Therefore, it is necessary to develop rapid and sensitive methods for identifying the chemical constituents of TCM prescriptions, to facilitate their quality control, safe and effective use in clinic.

Wuzi-Yanzong-Wan (WZYZW), the most classical Chinese herb prescription for nourishing the kidney and strengthening the essence, is called “ancient and modern seed first choice prescription”, being firstly detailed recorded in She-sheng-zhong-miao-fang treatise of the Ming Dynasty and widely used to treat syndrome of kidney deficiency and damage of essence, including impotence, sterility, spermatorrhea, premature ejaculation, lumbago and post-micturation dribble [4,5,6]. WZYZW is composed of Fructus Lych, Semen Cuscutae (fried), Fructus Rubi, Fructus Schi-sandrae chinensis (steamed), and Semen Plantaginis (fried with salt).

Over the years, the chemical work on WZYZW has mainly focused on either qualitative or quantitative analyses of its major components using different techniques [7,8,9,10], such as HPLC, LC-MS, etc. These methods have contributed to the study of WZYZW to some extent, but there has been no systematic characterization of the chemical constituents in this prescription, which could contribute to interpret the material basis for its therapeutic effects.

The comprehensive analysis of the chemical constituents in Chinese herbal prescriptions and herbal medicines is hard work, on account of the fact they typically include extraordinarily complex components. LTQ-Orbitrap-MS as a high resolution mass spectrometry technique has proven to be an advanced, accurate and reliable tool for the comprehensive identification of compounds in complex systems such as TCMs [11,12,13,14,15]. It has high sensitivity, resolution and mass accuracy, which provides multi-stage MS analysis (MSⁿ) information, acquisition of precursor ion data of high-resolution, determination of accurate mass charge ratios, structural information of fragment ions from MSⁿ, and gives the elementary composition of compounds and allows the structure confirmation of trace components in complicated samples [16]. A C18 column with a 1.7 µm particle pore size is employed for UPLC, resulting in higher peak capacity, greater resolution, and faster and more sensitive separations in comparison with HPLC [17,18]. Therefore, the present study is aimed at establishing a rapid and sensitive method for making the constituents of WZYZW more clear and comprehensible, using the UPLC-LTQ-Orbitrap-MS technique in both negative and positive modes. As a result, a total of 106 constituents were screened and identified, and the developed method was proved efficient, giving accurate and information-rich results. To our knowledge, this is the first time a comprehensive analysis of the multiple class compounds of WZYZW has been reported.

2. Results and Discussion

2.1. Optimization of Analytical Conditions

To obtain better chromatographic separation and mass spectrometric detection, four mobile phase systems: methanol-aqueous solution, acetonitrile-aqueous solution, acetonitrile-formic acid aqueous solution and acetonitrile containing formic acid-formic acid aqueous solution were examined, the acetonitrile containing formic acid-formic acid aqueous solution showed a better degree of separation. Formic acid (0.1%) was added to the mobile phase to enhance the intensity of mass signals and improve the peak shape. In addition, the flow rate (0.25, 0.3, 0.35 mL/min), column temperature (25, 30, 40 °C) and injection volume (2, 3, 5 uL) were respectively optimized in this work. The chromatographic separation was better when the chromatographic conditions were as follows: acetonitrile containing formic acid-aqueous formic acid solution, flow rate of 0.3 mL/min, column temperature of 30 °C and injection volume of 3 μL.

2.2. Chemical Constituents Identification in WZYZW

Chemical profiles of WZYZW in both negative and positive modes were well separated and detected by using the established UPLC-ESI-LTQ-Orbitrap-MS method. The TIC chromatograms in both ESI modes are shown in Figure 1.

In this study, for the compounds with available standards, the compound was identified by comparing the retention time and high-resolution accurate mass with the reference compound. Thus peaks 1, 5, 6, 10, 14, 17, 19, 20, 21, 27, 36, 51, 59, 60, 61, 64, 65, 68 and 75 were unambiguously identified as abromine, nicotinic acid, thiamine, riboflavin, taurine, quinic acid, atropine, ferulic acid, chlorogenic acid, scopoletin, rutin, esculin, apigenin, hesperidin, quercetin, kaempferol, luteolin, isorhamnetin and schisandrin, respectively. Meanwhile, the fragmentation patterns and pathways of the standards helped further confirm the structures of the derivatives of the reference compounds. When no standard compounds were available, the structures were elucidated by adopting the following measures to improve the quality of the identifications: firstly, based on the high-accuracy precursor ions obtained from the LTQ-Orbitrap-MS, using Xcalibur 2.1, the element compositions were calculated following some rules (C = 40, H = 100, O = 30, N = 5, Na = 2 and RDB equivalent value = 15). The maximum tolerance of mass error for all the precursor and product ions was set at 5 ppm, which can satisfy the requirements for positive identification. Besides, the most rational molecular formula was sought in different chemical databases such as the Spectral Database for Organic Compounds SDBS (http://sdbs.db.aist.go.jp) and Massbank (http://www.massbank.jp). When several matching compounds with the same formula were found, the structures were sought in the chemical information of the compounds from the five crude drugs of WZYZW. The component herb from which each compound was derived was confirmed by individually analyzing the herbs with the same UPLC-LTQ-Orbitrap-MS method. Finally, the chemical structures were further confirmed by combining the chromatographic data, the high resolution and accurate mass product ion information provided by data-dependent scan from MSⁿ experiments and referring to literature references. Moreover, the fragmentation patterns and pathways of the standards contributed to further confirming the derivatives of the reference compounds, then, the structure could be tentatively identified. A total of 106 compounds of WZYYW were thus identified or tentatively identified, including 35 flavonoids, 34 phenylpropanoids, 17 organic acids, 8 alkaloids, 11 terpenoids and one miscellaneous ingredient; 14 ingredients from Fructus Lych were identified, 10 ingredients from Semen Cuscutae (fried), 33 ingredients from Fructus Rubi, 37 ingredients from Fructus Schisandrae chinensis (steamed), and 20 ingredients from Semen Plantaginis (fried with salt). Table 1 summarizes their information.

Figure 1. Total ion chromatogram (TIC) of WZYZW in negative ion mode (a) and positive ion mode (b) using UPLC-ESI-LTQ-Orbitrap-MS.

Figure 1. Total ion chromatogram (TIC) of WZYZW in negative ion mode (a) and positive ion mode (b) using UPLC-ESI-LTQ-Orbitrap-MS.

Table 1. Identification of the chemical constituents of WZYZW by UPLC-ESI-LTQ-Obitrap-MS.

No.	tR (min)	Molecular Formula	Caculated Mass (m/z)	Experimental Mass (m/z)		MS/MS Fragments		Identification Compound	Source
				N-ion	ppm	P-ion	ppm
1	0.81	C5H11NO2	117.08626	―	―	118.08569	−0.6	235[2M + H]+;	Abromine	a
								118[M + H]+;
								59[C3H9N]+;
								58[C3H8N]+
2	0.87	C6H8O6	176.02371	175.02289	−4.7	―	―	175[M − H]−;	Ascorbic acid	a
								115[C4H3O4]−;
								113[C5H5O3]−
3	1.12	C6H8O7	192.01863	191.0189	1.4	―	―	191[M − H]−;	Citric acid	d
								173[M − H − H2O]−;
								129[M − H − H2O − CO2]−;
								111[M − H − H2O − COOH − OH]−
4	1.12	C4H6O5	134.01315	133.01372	4.3	―	―	133[M + H]+;	Malic acid	d
								115[M + H − H2O]+
5	1.28	C6H5NO2	123.03931	122.02417	4.2	124.03876	−4.3	122[M − H]−;	Nicotinic acid	a
								124[M + H]+;
								106 [M + H − H2O]+
6	1.58	C12H16N4OS	264.11176	―	―	265.11071	−3.9	265[M + H]+;	Thiamine	a
								156[C7H10NOS]+;
								144[C6H10NOS]+;
								122[C6H8N3]+
7	1.97	C4H6O4	118.01824	117.01791	−2.8	―	―	117[M − H]−;	Succinic acid	d
								99[M − H − H2O]−;
								73[M − H − CO2]−
8	2.68	C7H6O5	170.01315	169.01301	−0.8	―	―	169[M − H]−;	Gallic acid	c
								125[M – H − CO2]−
9	3.08	C4H6O6	150.00806	149.00808	0.1	―	―	149[M − H]−;	Tartaric acid	d
								131[M − H − H2O]−;
								103[M − H − HCOOH]−
10	3.28	C17H20N4O6	376.12991	375.12988	−0.1	―	―	375[M − H]−;	Riboflavin	a
								255[C13H11N4O2]−;
								212[C12H10N3O]−
11	4.48	C7H6O3	138.02332	137.02365	2.4	―	―	137[M − H]−;	Salicylic acid/	c
								93[C6H5O]−	4-Hydroxyben-zoic acid
12	4.66	C7H6O4	154.01824	153.01862	2.5	―	―	153[M − H]−;	Protocatechuic acid	d
								109[M − H − CO2]−
13	4.74	C7H6O3	138.02332	137.02373	3	―	―	137[M − H]−;	Salicylic acid/	c
								93[C6H5O]−	4-Hydroxyben-zoic acid
14	7.2	C2H7NO3S	125.00629	124.00638	0.7	―	―	124[M − H]−;	Taurine	a
								80[SO3]−
15	7.38	C8H8O4	168.03389	167.0342	1.9	―	―	167[M − H]−;	Vanillic acid	a,c
								152[M − H − CH3]−;
								123[M − H − CO2]−;
								108[M − H − CH3 − CO2]−
16	8.35	C16H22O10	374.11293	373.11377	2.3	397.10892	−4.0	373[M − H]−;	Geniposidic acid	e
								329[M − H − CO2]−;
								211[M − H − Glc]−;
								193 [M − H − Glc − H2O]−;
								167[M − H − Glc − CO2]−;
								149[M − H − Glc − CO2 − H2O]−;
								397[M + Na]+;
								353[M + Na − CO2]+;
								235[M + Na − Glc]+;
								217[M + Na − Glc − H2O]+;
								149[C7H10O2Na]+
17	8.79	C7H12O6	192.05502	191.05503	0.1	193.06981	−4.4	191[M − H]−;	Quinic acid	d
								173[M − H − H2O]−;
								155[M − H − 2H2O]−;
								127[M − H − 2H2O − CO]−;
								193 [M + H]+;
								175[M + H − H2O]+;
								157[M + H − 2H2O]+
18	9.68	C9H8O4	180.03389	179.03429	2.2	181.04889	−3.5	179[M − H]−;	Caffeic acid	a
								135[M − H − CO2]−;
								181[M + H]+;
								163[M + H − H2O]+;
								145[M + H − 2H2O]+
19	10.2	C17H23NO3	289.17507	―	―	290.17578	2.4	290[M + H]+;	Atropine	a
								272[M + H − H2O]+;
								260[M + H − HCHO]+;
								242[M + H − HCHO − H2O]+;
								124[C8H14N]+;
								95[C7H11]+
20	11.91	C10H10O4	194.04954	193.05001	2.4	195.06447	−3.7	193[M − H]−;	Ferulic Acid	a,c
								175[M − H − H2O]−;
								149[M − H − CO2]−;
								134[M − H − CH3 − CO2]−;
								195[M + H]+;
								177[M + H − H2O]+;
								145[M + H − H2O − CH3 − OH]+
21	12.37	C16H18O9	354.08671	353.08731	1.7	355.10097	−3.9	353[M − H]−;	Chlorogenic acid	a
								191[C7H11O6]−;
								173[C7H9O5]−;
								127[C6H7O3]−;
								355[M + H]+;
								163[C9H7O3]+;
								117[C8H5O]+
22	13.1	C9H8O3	164.03897	163.03905	0.5	165.05393	−4.2	163[M − H]−;	p-Coumaric acid	a
								119[M − H − CO2]−;
								165 [M + H]+;
								147[M + H − H2O]+
23	13.64	C7H10O5	174.04445	173.04381	−3.7	―	―	173[M − H]−;	Shikimic acid	c
								155[M − H − H2O]−;
								137[M − H − 2H2O]−;
								128[M − H − COOH]−
24	13.97	C27H30O17	626.24908	625.25148	3.8	―	―	625[M − H]−;	Quercetin-3-O-β-d-galactopyra-noside-7-O-β-d-glucopyranoside	b
								463[M − H − Gal]−;
								301[M − H − Gal − Glc]−;
								300[M − H − Gal − Glc − H]−;
								273[M − H − Gal − Glc − CO]−;
								255[M − H − Gal − Glc − CO − H2O]−
25	15.6	C9H6O4	178.01824	177.01847	1.3	179.03317	−4.0	177[M − H]−;	Esculetin	c
								149[M − H − CO]−;
								133[M − H − CO2]−;
								121[M − H − 2CO]−;
								105[M − H − CO2 − CO]−;
								93[M − H − 3CO]−;
								179[M + H]+;
								151[M + H − CO]+;
								133[M + H − CO − H2O]+
26	17.17	C22H22O11	462.10784	461.10962	3.9	―	―	461[M − H]−;	Homoplantagini-n	e
								299[M − H − Glu]−;
								271[M − H − Glu − CO]−;
								181[C8H5O5]−
27	19.43	C10H8O4	192.03387	191.03419	1.6	193.04933	−1.1	191[M − H]−;	Scopoletin	a
								193[M + H]+;
								178[M + H − CH3]+;
								165[M + H − CO]+;
								161[M + H − CH3OH]+;
								133[M + H − CH3OH − CO]+
28	19.6	C21H20O12	464.08711	463.08829	2.6	―	―	463[M − H]−;	6-Hydroxy-luteolin-7-O-glucoside	e
								301[M − H − Glc]−;
								273[M − H − Glc − CO]−;
								257[M − H − Glc − CO2]−;
								167[C7H3O5]−
29	20.93	C22H20O12	476.0871	475.08633	−1.6	―	―	475[M − H]−;	Kaempferol-3-O-β-d-glucuronic acid methyl ester	c
								457[M − H − H2O]−;
								433[M − H − CO=CH2]−;
								415[M − H − CH3COOH]−;
								285[M − H − C7H10O6]−
30	21.62	C15H10O7	302.03428	301.03396	−1.1	―	―	301[M − H]−;	6-Hydroxy-luteolin	e
								273[M − H − CO]−;
								257[M − H − CO2]−;
								167[C7H3O5]−
31	21,78	C26H28O16	596.14463	595.14543	1.4	―	―	595[M − H]−;	Quercetin-3-O-β-d-galactosyl-(1→2)-β-d-apioside	b
								505[M − H – Api + C2H2O]−;
								463[M − H − Api]−;
								445[M − H − Api − H2O]−;
								301[M − H − Api − Gal]−;
								300[M − H − Api − Gal − H]−;
								273[M − H − Api − Gal − CO]−;
								257[M − H − Api − Gal − 2CO]−;
								179[C8H3O5]−
32	21.88	C14H6O8	302.01355	―	―	303.01321	−1.1	303[M+H]+;	Ellagic acid	c
								285[M + H − H2O]+;
								275[M + H − CO]+;
								257[M + H − CO − H2O]+;
								247[M + H − 2CO]+;
								201[C11H5O4]+
33	22.26	C29H28O9	520.18061	―	―	521.18042	−0.4	521[M + H]+;	Schisantherin D	d
								399[M + H − C6H5COOH]+;
								355[M + H − C6H5COOH − C2H4O]+
34	22.9	C21H20O12	464.08711	463.08887	3.8	―	―	463[M − H]−;	Hyperoside	b,c
								301[M − H − Gal]−;
								255[M − H − Gal − CO − H2O]−;
								151[C7H3O4]−;
								107[C6H3O2]−
35	23.02	C16H12O6	300.05502	299.05453	−1.6	―	―	299[M − H]−;	Hispidulin	e
								271[M − H − CO]−;
								255[M − H − CO2]−;
								227[M − H − CO − CO2]−;
								181[C8H5O5]−
36	23.1	C27H30O16	610.14501	609.1479	4.7	―	―	609[M − H]−;	Rutin	c
								343[C17H11O8]−;
								301[M − H − Rha − Glc]−;
								300[M − H − Rha − Glc − H]−;
								271[M − H − Rha − Glc − H − CO − H]−;
								255[M − H − Rha − Glc − H − CO − OH]−;
								179[C8H3O5]−;
								151[C7H3O4]−
37	23.74	C21H20O12	464.08711	463.0874	0.6	―	―	463[M − H]−;	Isoquercitrin	c
								301[M − H − Glc]−;
								255[M − H − Glc − CO − H2O]−;
								151[C7H3O4]−;
								107[C6H3O2]−
38	24.2	C29H36O16	640.19197	639.19263	1	―	―	639[M − H]−;	Plantamajoside	e
								477[M − H − C9H6O3]−;
								315[M − H − C9H6O3 − Glc]−;
								153[M − H − C9H6O3 − 2Glc]−;
								135[M − H − C9H6O3 − 2Glc − H2O]−
39	24.95	C27H30O15	594.15009	593.1507	1	―	―	593[M − H]−;	Kaempferol-3-O-rutinoside	c
								327[M − H − C10H18O8]−;
								285[M − H − Rha − Glc]−;
								284[M − H − Rha − Glc − H]−;
								257[M − H − Rha − Glc − CO]−;
								255[M − H − Rha − Glc − H − CO − H]−;
								229[M − H − Rha − Glc − 2CO]−;
								227[M − H − Rha − Glc − H − 2CO − H]−
40	25.34	C28H36O8	500.24829	―	―	501.25002	3.4	501[M + H]+;	Tigloylgomisin H/Angeloygomi-sin H	d
								484[M + H − OH]+;
								483[M + H − H2O]+;
								384[M + H − OH − C4H7COOH]+;
								357[M + H − C4H7COOH − C2H4O]+
41	25.95	C21H20O11	448.09219	447.09378	3.6	―	―	447[M − H]−;	Luteoloside	e
								285[M − H − Glc]−;
								284[M − H − Glc − H]−;
								257[M − H − Glc − CO]−;
								243[M − H − Glc − C2H2O]−;
								241[M − H − Glc − CO2]−;
								199[M − H − Glc − C2H2O − CO2]−;
								197[M − H − Glc − 2CO2]−;
								151[C7H3O4]−
42	26.1	C28H36O8	500.24829	―	―	501.25071	4.8	501[M + H]+;	Tigloylgomisin H/Angeloygomi-sin H	d
								484[M + H − OH]+;
								483[M + H − H2O]+;
								384[M + H − OH − C4H7COOH]+;
								357[M + H − C4H7COOH − C2H4O]+
43	26.57	C29H36O15	624.19707	623.19604	−1.6	―	―	623[M − H]−;	Acteoside	e
								461[M − H − C9H6O3]−;
								315[M − H − C9H6O3 − Rha]−;
								179[C9H7O4]−;
								153[M − H − C9H6O3 − Rha − Glc]−;
								135[M − H − C9H6O3 − Rha − Glc − H2O]−
44	27.19	C21H20O11	448.09219	447.09225	0.1	449.10532	−2.5	447[M − H]−;	Astragalin	b,c
								285[M − H − Glc]−;
								151[C7H3O4]−;
								449[M + H]+;
								287[M − H − Glc]+;
								241[M − H − Glc − CH2O2]+;
								213[M + H − Glc − C2H2O3]+
45	27.19	C21H20O11	448.09219	447.09225	0.1	449.10699	−1.9	447[M − H]−;	Plantaginin	e
								285[M − H − Glc]−;
								267[M − H − Glc − H2O]−;
								255[M − H − Glc − CH2O]−;
								239[M − H − Glc − H2O − CO]−;
								167[C7H3O5]−;
								449[M + H]+;
								287[M+H − Glc]+;
								269[M + H − Glc − H2O]+;
								169[C7H5O5]+
46	27.48	C29H36O15	624.19705	623.19794	1.4	625.21005	−4.2	623[M − H]−;	Methylhesperidin	c
								477[M − H − Rha]−;
								315[M − H − Rha − Glc]−;
								300[M − H − Rha − Glc − CH3]−;
								297[M − H − Rha − Gldc − H2O]−;
								285[M − H − Rha − Glc − 2CH3]−;
								282[M − H − Rha − Glc − H2O − CH3]−;
								272[M − H − Rha − Glc − CH3 − CO]−;
								257[M − H − Rha − Glc − 2CH3 − CO]−;
								229[M − H − Rha − Glc − 2CH3 − 2CO]−;
								625[M + H]+;
								479[M + H − Rha]+;
								317[M + H − Rha − Glc]+;
								299[M + H − Rha − Glc − H2O]+;
								281[M + H − Rha − Glc − 2H2O]+;
								193[C10H9O4]+;
								165[C9H9O3]+
47	27.72	C21H20O11	448.09219	447.09402	4.1	―	―	447[M − H]−;	Quercitrin	c
								301[M − H − Rha]−;
								300[M − H − Rha − H]−;
								273[M − H − Rha − CO]−;
								255[M − H − Rha − CO − H2O]−
48	27.86	C22H22O12	478.10275	477.10461	3.9	―	―	477[M − H]−;	Nepetin-7-O-glucoside	e
								315[M − H − Glc]−;
								287[M − H − Glc − CO]−;
								271[M − H − Glc − CO2]−;
								181[C8H5O5]−
49	27.93	C21H20O10	432.09727	431.09805	1.8	433.11093	−4.6	431[M − H]−;	Kaempferol-7-O-α-l-rhamnoside	c
								413[M − H − H2O]−;
								285[M − H − Rha]−;
								267[M − H − Rha − H2O]−;
								169[C12H9O]−;
								155[C11H7O]−;
								433[M + H]+
50	27.97	C21H20O10	432.09727	431.09866	3.2			431[M − H]−;	Apigenin-7-O-glucoside	e
								269[M − H − Glc]−;
								201[M − H − Glc − C3O2]−;
								151[C7H3O4]−
51	27.99	C15H16O9	340.07106	339.07058	−1.4	―	―	339[M − H]−;	Esculin	c
								177[M − H − Glc]−;
								133[M − H − Glc − CO2]−;
								105[M − H − Glc − CO2 − CO]−;
								89[C7H5]−
52	28.28	C27H30O14	578.15518	577.15662	2.5	579.17073	−0.2	577[M − H]−;	Rhoifolin	e
								457[M − H − C4H8O4]−;
								431[M − H − Rha]−;
								413[M − H − Rha − H2O]−;
								269[M − H − Rha − Glc]−;
								225[M − H − Rha − Glc − CO2]−;
								183[C12H7O2]−;
								579[M + H]+;
								433[M + H − Rha]+;
								415[M + H − Rha − H2O]+;
								397[M + H − Rha − 2H2O]+;
								271[M + H − Rha − Glc]+;
								253[M + H − Rha − Glc − H2O]+;
								243[M + H − Rha − Glc − CO]+;
								225[M + H − Rha − Glc − H2O − CO]+;
								211[M + H − Rha − Glc − H2O − C2H2O]+;
								153[C7H5O4]+;
								119[C8H7O]+;
								91[C7H7]+
53	28.28	C28H32O16	624.16066	623.16248	2.9	625.17896	4.2	623[M − H]−;	Isorhamnetin-3-O-β-d-rutinoside	a
								477[M − H − Rha]−;
								315[M − H − Rha − Glc]−;
								300[M − H − Rha − Glc − CH3]−;
								272[M − H − Rha − Glc − CH3 − CO]−;
								271[M − H − Rha − Glc − CH3 − CO − H]−;
								243[M − H − Rha − Glc − CH3 − CO − H − CO]−;
								227[M − H − Rha − Glc − CH3 − CO − H − CO2]−;
								151[C7H3O4]−;
								625[M + H]+;
								479[M + H − Rha]+;
								317[M + H − Rha − Glc]+;
								302[M + H − Rha − Glc − CH3]+;
								285[M + H − Rha − Glc − CH3OH]+;
								274[M + H − Rha − Glc − CH3 − CO]+;
								257[M + H − Rha − Glc − CH3OH − CO]+;
								246[M + H − Rha − Glc − CH3 − 2CO]+;
								229[M + H − Rha − Glc − CH3OH − 2CO]+;
								153[C7H5O4]+
54	28.31	C21H18O11	446.07654	445.07493	−3.6	―	―	445[M − H]−;	Baicalin	e
								269[M − H − GluA]−;
								225[M − H − GluA − CO2]−;
								167[C7H3O5]−
55	29.09	C20H26O4	330.19039	―	―	331.18914	−3.8	331[M + H]+;	Meso-dihydrog-uaiaretic acid	d
								313[M + H − H2O]+;
								301[M + H − 2CH3]+
56	29.58	C15H10O5	270.0601	269.09566	−1.6	271.05966	−1.7	269[M − H]−;	Baicalein	e
								241[M − H − CO]−;
								225[M − H − CO2]−;
								197[M − H − CO − CO2]−;
								167[C7H3O5]−;
								271[M + H]+;
								253[M + H − H2O]+;
								169[C7H5O5]+
57	30.7	C28H34O15	610.1814	609.18229	1.5	611.1994	3.9	609[M − H]−;	Neohesperidin	c
								505[M − H − C4H8O3]−;
								463[M − H − Rha]−;
								445[M − H − Rha − H2O]−;
								427[M − H − Rha − 2H2O]−;
								301[M − H − Rha − Glc]−;
								286[M − H − Rha − Glc − CH3]−;
								283[M − H − Rha − Glc − H2O]−;
								268[M − H − Rha − Glc − H2O − CH3]−;
								258[M − H − Rha − Glc − CH3 − CO]−;
								257[M − H − Rha − Glc − CO2]−;
								242[M − H − Rha − Glc − CH3 − CO2]−;
								239[M − H − Rha − Glc − H2O − CO2]−;
								199[C12H7O3]−;
								125[C6H5O3]−;
								611[M + H]+;
								303[M + H − Rha − Glc]+;
								285[M + H − Rha − Glc − H2O]+;
								179[C9H7O4]+;
								177[C10H9O3]+;
								151[C8H7O3]+
58	31.48	C23H28O8	432.1857	―	―	433.18538	−0.7	433[M + H]+;	Schizandradiol	d
								415[M + H − H2O]+;
								361[M + H − C4H8O]+;
								372[M + H − H2O − CH3CO]+;
								343[M + H − H2O − C4H7OH]+
59	31.54 39.44	C15H10O5	270.04445	269.04353	−3.4	271.06145	4.9	269[M − H]−;	Apigenin	e
								241[M − H − CO]−;
								227[M − H − C2H2O]−;
								225[M − H − CO2]−;
								201[M − H − C3O2]−;
								151[C7H3O4]−;
								271[M + H]+;
								253[M + H − H2O]+;
								225[M + H − H2O − CO]+
60	31.71	C28H34O15	610.1814	609.18351	3.5	611.20001	4.8	609[M − H]−;	Hesperidin	c
								463[M − H − Rha]−;
								301[M − H − Rha − Glc]−;
								286[M − H − Rha − Glc − CH3]−;
								283[M − H − Rha − Glc − H2O]−;
								268[M − H − Rha − Glc − H2O − CH3]−;
								258[M − H − Rha − Glc − CH3 − CO]−;
								257[M − H − Rha − Glc − CO2]−;
								242[M − H − Rha − Glc − CH3 − CO2]−;
								239[M − H − Rha − Glc − H2O − CO2]−;
								199[C12H7O3]−;
								125[C6H5O3]−;
								611[M + H]+;
								303[M + H − Rha − Glc]+;
								285[M + H − Rha − Glc − H2O]+;
								179[C9H7O4]+;
								177[C10H9O3]+;
								151[C8H7O3]+
61	32.13	C15H10O7	302.03428	301.03549	4	303.0488	−1.1	301[M − H]−;	Quercetin	b,c
								273[M − H − CO]−;
								255[M − H − CO − H2O]−;
								151[C7H3O4]−;
								303[M + H]+;
								285[M + H − H2O]+;
								257[M + H − H2O − CO]+;
								247[M + H − 2CO]+;
								229[M + H − H2O − 2CO]+
62	33.02	C30H26O13	594.12897	593.13049	2.6	595.14142	−3.2	593[M − H]−;	Tiliroside	c
								447[M − H − C9H6O2]−;
								285[M − H − C9H6O2 − Glc]−;
								257[M − H − C9H6O2 − Glc − CO]−;
								229[M − H − C9H6O2 − Glc − 2CO]−;
								151[C7H3O4]−;
								617[M + Na]+;
								595[M + H]+;
								449[M + H − C9H6O2]+;
								287[M + H − C9H6O2 − Glc]+
63	34.12	C15H12O6	288.05502	287.05549	1.7	289.07002	−2.2	287[M − H]−;	Aromadendrin	c
								269[M − H − H2O]−;
								259[M − H − CO]−;
								243[M − H − CO2]−;
								225[M − H − H2O − CO2]−;
								215[M − H − CO − CO2]−;
								151[C7H3O4]−;
								107[C6H3O2]−;
								289[M + H]+;
								271[M + H − H2O]+;
								195[C9H7O5]+;
								145[C9H5O2]+
64	35.18	C15H10O6	286.03936	285.03995	2.1	287.05359	4.9	285[M − H]−;	Kaempferol	b,c
								257[M − H − CO]−;
								229[M − H − 2CO]−;
								151[C7H3O4]−;
								309[M + Na]+;
								287[M + H]+;
								241[M + H − CH2O2]+;
								213[M + H − C2H2O3]+
65	35.31	C15H10O6	286.03936	285.04019	2.9	―	―	571[2M − H]−;	Iuteolin	e
								285[M − H]−;
								243[M − H − C2H2O]−;
								241[M − H − CO2]−;
								199[M − H − C2H2O − CO2]−;
								197[M − H − 2CO2]−;
								171[M − H − C2H2O − CO2 − CO]−;
								151[C7H3O4]−
66	35.58	C16H12O7	316.04993	315.05117	3.9	―	―	315[M − H]−;	Nepetin	e
								287[M − H − CO]−;
								271[M − H − CO2]−;
								243[M − H − CO − CO2]−;
								181[C8H5O5]−
67	35.61	C15H10O6	286.03937	285.03989	1.8	287.05627	4.4	285[M − H]−;	Scutellarein	e
								257[M − H − CO]−;
								241[M − H − CO2]−;
								167[C7H3O5]−;
								287[M + H]+;
								269[M + H − H2O]+;
								241[M + H − H2O − CO]+;
								169[C7H5O5]+
68	35.61	C16H12O7	316.04993	315.05027	1.1	317.06486	−2.3	315[M − H]−;	Isorhamnetin	b
								300[M − H − CH3]−;
								272[M − H − CH3 − CO]−;
								271[M − H − CH3 − CO − H]−;
								243[M − H − CH3 − CO − H − CO]−;
								227[M − H − CH3 − CO − H − CO2]−;
								151[C7H3O4]−;
								107[C6H3O2]−;
								339[M + Na]+;
								317[M + H]+;
								302[M + H − CH3]+;
								285[M + H − CH3OH]+;
								274[M + H − CH3 − CO]+;
								257[M + H − CH3OH − CO]+;
								246[M + H − CH3 − 2CO]+;
								229[M + H − CH3OH − 2CO]+;
								153[C7H5O4]+
69	35.76	C30H46O4	470.34689	469.33251	2.7	471.34509	−3.8	469[M − H]−;	Nigranoic acid	d
								423[M − H − HCOOH]−;
								378[M − H − HCOOH − HCOO]−;
								471[M + H]+;
								456[M + H − CH3]+;
								453[M + H − H2O]+;
								390[M + H − C6H9]+
70	35.89	C28H24O16	616.09807	615.10097	4.7	―	―	615[M − H]−;	2″-O-Galloylhyperoside	c
								463[M − H − C7H4O4]−;
								445[M − H − C7H6O5]−;
								301[M − H − C7H4O4 − Glc]−
71	36.79	C23H30O7	418.20643	―	―	419.20477	−4.0	419[M + H]+;	Gomisin H	d
								401[M + H − H2O]+;
								384[M + H − OH − H2O]+;
								383[M + H − 2H2O]+;
								369[M + H − OH − CH3 − H2O]+;
								353[M + H − OH − OCH3 − H2O]+
72	37.08	C28H34O8	498.23264	―	―	499.23119	−2.9	499[M + H]+;	Angeloygomisin O/Angeloylisogomisin O	d
								399[M + H − C4H7COOH]+;
								369[M + H − C4H7COOH − CH2O]+;
								357[M + H − C4H7COOH − C3H6]+;
								343[M + H − C4H7COOH − C4H8]+
73	38.12	C28H34O8	498.23264	―	―	499.23026	−4.8	499[M + H]+;	Angeloygomisin O/Angeloylisogomisin O	d
								399[M + H − C4H7COOH]+;
								369[M + H − C4H7COOH − CH2O]+;
								357[M + H − C4H7COOH − C3H6]+;
								343[M + H − C4H7COOH − C4H8]+
74	39.28	C23H28O7	416.19078	―	―	417.18903	−4.2	417[M + H]+;	Gomisin O/Epigomisin O	d
								402[M + H − CH3]+;
								399[M + H − H2O]+;
								385[M + H − CH3OH]+;
								373[M + H − C2H4O]+
75	39.38	C24H32O7	432.22209	―	―	433.22103	−2.4	433[M + H]+;	Schisandrin	d
								415[M + H − H2O]+;
								400[M + H − H2O − CH3]+;
								384[M + H − H2O − OCH3]+;
								373[M + H − H2O − C3H6]+;
								359[M + H − H2O − C4H8]+
76	39.44	C30H46O4	470.34689	―	―	471.34551	−2.9	471[M + H]+;	Kadsuric acid	d
								453[M + H − H2O]+;
								398[M + H − CH2CH2COOH]+;
								370[M + H − CH2CH2COOH − C2H4]+
77	39.6	C23H28O7	416.19078	―	―	417.19103	0.6	417[M + H]+;	Gomisin O/Epigomisin O	d
								402[M + H − CH3]+;
								399[M + H − H2O]+;
								385[M + H − CH3OH]+;
								373[M + H − C2H4O]+
78	40.63	C28H34O10	530.22247	―	―	531.22218	−0.6	531[M + H]+;	Gomisin D	d
								485[M + H − CH2O2]+;
								401[M + H − C6H10O3]+;
								383[M + H − C6H10O3 − H2O]+
79	40.98	C22H28O6	388.19586	―	―	389.19503	−2.1	389[M + H]+;	Gomisin J	d
								374[M + H − CH3]+;
								357[M + H − CH3OH]+;
								342[M + H − CH3OH − CH3]+
80	41.02	C30H48O5	488.3418	487.3439	4.3	489.35526	−4.5	487[M − H]−;	Rosolic acid/2α,3α,19α-Trihydroxyolean-12-ene-28-oic acid/Arjunolic acid	c
								469[M − H − H2O]−;
								443[M − H − CO2]−;
								425[M − H − CO2 − H2O]−;
								407[M − H − CO2 − 2H2O]−;
								391[M − H − CO2 − 2H2O − CH4]−;
								489[M + H]+;
								453[M + H − 2H2O]+
81	41.22	C30H48O5	488.3418	487.34378	4	489.35544	−4.1	487[M − H]−;	Rosolic acid/2α,3α,19α-Trihydroxyolean-12-ene-28-oic acid/Arjunolic acid	c
								469[M − H − H2O]−;
								443[M − H − CO2]−;
								425[M − H − CO2 − H2O]−;
								407[M − H − CO2 − 2H2O]−;
								391[M − H − CO2 − 2H2O − CH4]−;
								489[M + H]+;
								453[M + H − 2H2O]+
82	41.75	C15H24N2O2	264.10889	―	―	265.18997	−4.0	265[M + H]+;	Sophoranol	b
								248[M + H − OH]+;
								247[M + H − H2O]+
83	42.18	C30H46O3	454.35198	―	―	455.35025	−3.8	455[M + H]+;	Ganwuweizic acid	d
								409[M + H − HCOOH]+;
								313[M + H − C8H14O2]+
84	42.32	C30H48O5	488.3418	487.34302	2.5	489.35529	−4.4	487[M − H]−;	Rosolic acid/2α,3α,19α-Trihydroxyolean-12-ene-28-oic acid/Arjunolic acid	c
								469[M − H − H2O]−;
								443[M − H − CO2]−;
								425[M − H − CO2 − H2O]−;
								407[M − H − CO2 − 2H2O]−;
								391[M − H − CO2 − 2H2O − CH4]−;
								489[M + H]+;
								453[M + H − 2H2O]+
85	42.89	C12H16N2O	204.13354	―	―	205.13409	2.6	205[M + H]+;	N-methylcytisine	b
								146[M + H − C3H9N]+;
								108[C6H6NO]+
86	43.27	C23H28O6	400.19587	―	―	401.19434	−3.8	401[M + H]+;	Schizandrin B	d
								386[M + H − CH3]+;
								370[M + H − OCH3]+;
								355[M + H − CH3 − OCH3]+;
								339[M + H − 2CH3 − CH3OH]+
87	43.3	C30H34O8	522.23265	―	―	523.23095	−3.2	523[M + H]+;	Benzoylgomisin H	d
								505[M + H − H2O]+;
								401[M + H − C6H5COOH]+;
								383[M + H − C6H5COOH − H2O]+
88	45.42	C23H30O6	402.21151	―	―	403.21011	−3.5	403[M + H]+;	Schisanhenol	d
								372[M + H − OCH3]+;
								371[M + H − CH3OH]+;
								356[M + H − CH3OH − CH3]+;
								340[M + H − OCH3 − CH3OH]+
89	45.44	C23H28O7	416.19078	―	―	417.18957	−2.9	417[M + H]+;	Schisandrol B	d
								399[M + H − H2O]+;
								343[M + H − C4H8 − H2O]+;
								307[M + H − 2CH3 − 2OCH3 − H2O]+
90	45.67	C28H34O9	514.22755	―	―	515.22559	−3.8	515[M + H]+;	Schisantherin B/Schisantherin C	d
								415[M + H − C4H7COOH]+;
								385[M + H − C4H7COOH − CH2O]+;
								367[M + H − C4H7COOH − CH2O − H2O]+;
								353[M + H − C4H7COOH − CH2O − CH3OH]+
91	46.06	C30H32O9	536.21191	―	―	537.20981	−3.9	537[M + H]+;	Schisantherin A	d
								415[M + H − C6H5COOH]+;
								397[M + H − C6H5COOH − H2O]+;
								373[M + H − C6H5COOH − C3H6]+
92	46.58	C30H48O4	472.34689	471.34872	3.9	473.30911	−3.7	471[M − H]−;	Corosolic acid/Maslinic acid	c
								453[M − H − H2O]−;
								427[M − H − CO2]−;
								391[M − H − CO2 − 2H2O]−;
								473[M + H]+;
								455[M + H − H2O]+;
								203[C15H23]+;
								105[C8H9]+
93	46.73	C15H24N2O	248.19614	―	―	249.19699	2.2	271[M + Na]+;	Matrine	b
								249[M+H]+;
								231[M + H − H2O]+;
								150[C10H16N]+;
								148[C10H14N]+
94	47.28	C22H26O6	386.18022	―	―	387.17992	−0.8	387[M + H]+;	Gomisin L1/Gomisin L2/Gomisin M1/Gomisin M2	d
								372[M + H − CH3]+;
								369[M + H − H2O]+;
								357[M + H − CH2O]+;
								329[M + H − C2H2O2]+
95	47.52	C30H48O4	472.34689	471.34863	3.7	473.36044	−4.4	471[M − H]−;	Corosolic acid/Maslinic acid	c
								453[M − H − H2O]−;
								427[M − H − CO2]−;
								391[M − H − CO2 − 2H2O]−;
								473[M + H]+;
								455[M + H − H2O]+;
								203[C15H23]+;
								105[C8H9]+
96	47.53	C22H26O6	386.18022	―	―	387.17847	−4.5	387[M + H]+;	Gomisin L1/Gomisin L2/Gomisin M1/Gomisin M2	d
								372[M + H − CH3]+;
								369[M + H − H2O]+;
								357[M + H − CH2O]+;
								329[M + H − C2H2O2]+
97	47.76	C28H34O9	514.22755	―	―	515.22716	−0.7	515[M + H]+;	Schisantherin B/Schisantherin C	d
								415[M + H − C4H7COOH]+;
								385[M + H − C4H7COOH − CH2O]+;
								367[M + H − C4H7COOH − CH2O − H2O]+;
								353[M + H − C4H7COOH − CH2O − CH3OH]+
98	48.01	C30H48O3	456.36763	455.35424	4.9	457.36636	−2.7	455[M − H]−;	Oleanolic acid/Ursolic acid	c,e
								407[M − H − HCHO − H2O]−;
								391[M − H − HCHO − H2O − CH4]−;
								479[M + Na]+;
								457[M + H]+;
								439[M + H − H2O]+;
								393[M + H − HCOOH − H2O]+
99	48.24	C22H26O6	386.18022	―	―	387.17883	−3.6	387[M + H]+;	Gomisin L1/Gomisin L2/Gomisin M1/Gomisin M2	d
								372[M + H − CH3]+;
								369[M + H − H2O]+;
								357[M + H − CH2O]+;
								329[M + H − C2H2O2]+
100	48.4	C30H48O3	456.36763	455.35394	4.3	457.36612	−3.3	455[M − H]−;	Oleanolic acid/Ursolic acid	c,e
								407[M − H − HCHO − H2O]−;
								391[M − H − HCHO − H2O − CH4]−;
								479[M + Na]+;
								457[M + H]+;
								457[M + H − H2O]+;
								393[M + H − HCOOH − H2O]+
101	48.65	C22H26O6	386.18022	―	―	387.17877	−3.7	387[M + H]+;	Gomisin L1/Gomisin L2/Gomisin M1/Gomisin M2	d
								372[M + H − CH3]+;
								369[M + H − H2O]+;
								357[M + H − CH2O]+;
								329[M + H − C2H2O2]+
102	49.79	C24H32O6	416.22714	―	―	417.22649	−1.6	417[M + H]+;	Deoxyschizan-drin	d
								402[M + H − CH3]+;
								386[M + H − OCH3]+;
								370[M + H − CH3 − CH3OH]+;
								347[M + H − CH3 − C4H7]+;
								316[M + H − C6H13O]+;
								286[M + H − C6H13O − 2CH3]+;
								273[M + H − C6H13O − CH3 − CO]+;
								227[M + H − C6H13O − CO − OCH3 − 2CH3]+
103	52.02	C16H14O4	270.09649	―	―	271.09595	−2.0	271[M + H]+; 203[M + H − C5H8]+;	Imperatorin	c
								175[M + H − C5H8 − CO]+;
								159[M + H − C5H8 − CO2]+
104	53.29	C30H32O8	520.21701	―	―	521.21559	−2.7	521[M + H]+;	Benzoylgomisin O/Benzoyisolgomisin O	d
								399[M + H − C6H5COOH]+;
								369[M + H − C6H5COOH − CH2O]+;
								357[M + H − C6H5COOH − C3H6]+;
								343 M + H − C6H5COOH − C4H8]+
105	53.43	C22H24O6	384.16457	―	―	385.16406	−1.3	385[M + H]+;	Schizandrin C	d
								370[M + H − CH3]+;
								355[M + H − CH2O]+
106	54.08	C30H32O8	520.21701	―	―	521.21611	−1.7	521[M + H]+;	Benzoylgomisin O/Benzoyisolgomisin O	d
								399[M + H − C6H5COOH]+;
								369[M + H − C6H5COOH − CH2O]+;
								357[M + H − C6H5COOH − C3H6]+;
								343 M + H − C6H5COOH − C4H8]+

a: Fructus Lych, b: Semen Cuscutae (fried), c: Fructus Rubi, d: Fructus Schisandrae chinensis (steamed), e: Semen Plantaginis (fried with salt); Glc : β-d-glucose, GluA: Glucuronic acid, Xyl: β-d-xylose, Rha: l-rhamnose; Gal: d-galactose; Api: d-apiose, ppm: difference between calculated and found mass.

Table 1. Identification of the chemical constituents of WZYZW by UPLC-ESI-LTQ-Obitrap-MS.

No.	tR (min)	Molecular Formula	Caculated Mass (m/z)	Experimental Mass (m/z)		MS/MS Fragments		Identification Compound	Source
				N-ion	ppm	P-ion	ppm
1	0.81	C5H11NO2	117.08626	―	―	118.08569	−0.6	235[2M + H]+;	Abromine	a
								118[M + H]+;
								59[C3H9N]+;
								58[C3H8N]+
2	0.87	C6H8O6	176.02371	175.02289	−4.7	―	―	175[M − H]−;	Ascorbic acid	a
								115[C4H3O4]−;
								113[C5H5O3]−
3	1.12	C6H8O7	192.01863	191.0189	1.4	―	―	191[M − H]−;	Citric acid	d
								173[M − H − H2O]−;
								129[M − H − H2O − CO2]−;
								111[M − H − H2O − COOH − OH]−
4	1.12	C4H6O5	134.01315	133.01372	4.3	―	―	133[M + H]+;	Malic acid	d
								115[M + H − H2O]+
5	1.28	C6H5NO2	123.03931	122.02417	4.2	124.03876	−4.3	122[M − H]−;	Nicotinic acid	a
								124[M + H]+;
								106 [M + H − H2O]+
6	1.58	C12H16N4OS	264.11176	―	―	265.11071	−3.9	265[M + H]+;	Thiamine	a
								156[C7H10NOS]+;
								144[C6H10NOS]+;
								122[C6H8N3]+
7	1.97	C4H6O4	118.01824	117.01791	−2.8	―	―	117[M − H]−;	Succinic acid	d
								99[M − H − H2O]−;
								73[M − H − CO2]−
8	2.68	C7H6O5	170.01315	169.01301	−0.8	―	―	169[M − H]−;	Gallic acid	c
								125[M – H − CO2]−
9	3.08	C4H6O6	150.00806	149.00808	0.1	―	―	149[M − H]−;	Tartaric acid	d
								131[M − H − H2O]−;
								103[M − H − HCOOH]−
10	3.28	C17H20N4O6	376.12991	375.12988	−0.1	―	―	375[M − H]−;	Riboflavin	a
								255[C13H11N4O2]−;
								212[C12H10N3O]−
11	4.48	C7H6O3	138.02332	137.02365	2.4	―	―	137[M − H]−;	Salicylic acid/	c
								93[C6H5O]−	4-Hydroxyben-zoic acid
12	4.66	C7H6O4	154.01824	153.01862	2.5	―	―	153[M − H]−;	Protocatechuic acid	d
								109[M − H − CO2]−
13	4.74	C7H6O3	138.02332	137.02373	3	―	―	137[M − H]−;	Salicylic acid/	c
								93[C6H5O]−	4-Hydroxyben-zoic acid
14	7.2	C2H7NO3S	125.00629	124.00638	0.7	―	―	124[M − H]−;	Taurine	a
								80[SO3]−
15	7.38	C8H8O4	168.03389	167.0342	1.9	―	―	167[M − H]−;	Vanillic acid	a,c
								152[M − H − CH3]−;
								123[M − H − CO2]−;
								108[M − H − CH3 − CO2]−
16	8.35	C16H22O10	374.11293	373.11377	2.3	397.10892	−4.0	373[M − H]−;	Geniposidic acid	e
								329[M − H − CO2]−;
								211[M − H − Glc]−;
								193 [M − H − Glc − H2O]−;
								167[M − H − Glc − CO2]−;
								149[M − H − Glc − CO2 − H2O]−;
								397[M + Na]+;
								353[M + Na − CO2]+;
								235[M + Na − Glc]+;
								217[M + Na − Glc − H2O]+;
								149[C7H10O2Na]+
17	8.79	C7H12O6	192.05502	191.05503	0.1	193.06981	−4.4	191[M − H]−;	Quinic acid	d
								173[M − H − H2O]−;
								155[M − H − 2H2O]−;
								127[M − H − 2H2O − CO]−;
								193 [M + H]+;
								175[M + H − H2O]+;
								157[M + H − 2H2O]+
18	9.68	C9H8O4	180.03389	179.03429	2.2	181.04889	−3.5	179[M − H]−;	Caffeic acid	a
								135[M − H − CO2]−;
								181[M + H]+;
								163[M + H − H2O]+;
								145[M + H − 2H2O]+
19	10.2	C17H23NO3	289.17507	―	―	290.17578	2.4	290[M + H]+;	Atropine	a
								272[M + H − H2O]+;
								260[M + H − HCHO]+;
								242[M + H − HCHO − H2O]+;
								124[C8H14N]+;
								95[C7H11]+
20	11.91	C10H10O4	194.04954	193.05001	2.4	195.06447	−3.7	193[M − H]−;	Ferulic Acid	a,c
								175[M − H − H2O]−;
								149[M − H − CO2]−;
								134[M − H − CH3 − CO2]−;
								195[M + H]+;
								177[M + H − H2O]+;
								145[M + H − H2O − CH3 − OH]+
21	12.37	C16H18O9	354.08671	353.08731	1.7	355.10097	−3.9	353[M − H]−;	Chlorogenic acid	a
								191[C7H11O6]−;
								173[C7H9O5]−;
								127[C6H7O3]−;
								355[M + H]+;
								163[C9H7O3]+;
								117[C8H5O]+
22	13.1	C9H8O3	164.03897	163.03905	0.5	165.05393	−4.2	163[M − H]−;	p-Coumaric acid	a
								119[M − H − CO2]−;
								165 [M + H]+;
								147[M + H − H2O]+
23	13.64	C7H10O5	174.04445	173.04381	−3.7	―	―	173[M − H]−;	Shikimic acid	c
								155[M − H − H2O]−;
								137[M − H − 2H2O]−;
								128[M − H − COOH]−
24	13.97	C27H30O17	626.24908	625.25148	3.8	―	―	625[M − H]−;	Quercetin-3-O-β-d-galactopyra-noside-7-O-β-d-glucopyranoside	b
								463[M − H − Gal]−;
								301[M − H − Gal − Glc]−;
								300[M − H − Gal − Glc − H]−;
								273[M − H − Gal − Glc − CO]−;
								255[M − H − Gal − Glc − CO − H2O]−
25	15.6	C9H6O4	178.01824	177.01847	1.3	179.03317	−4.0	177[M − H]−;	Esculetin	c
								149[M − H − CO]−;
								133[M − H − CO2]−;
								121[M − H − 2CO]−;
								105[M − H − CO2 − CO]−;
								93[M − H − 3CO]−;
								179[M + H]+;
								151[M + H − CO]+;
								133[M + H − CO − H2O]+
26	17.17	C22H22O11	462.10784	461.10962	3.9	―	―	461[M − H]−;	Homoplantagini-n	e
								299[M − H − Glu]−;
								271[M − H − Glu − CO]−;
								181[C8H5O5]−
27	19.43	C10H8O4	192.03387	191.03419	1.6	193.04933	−1.1	191[M − H]−;	Scopoletin	a
								193[M + H]+;
								178[M + H − CH3]+;
								165[M + H − CO]+;
								161[M + H − CH3OH]+;
								133[M + H − CH3OH − CO]+
28	19.6	C21H20O12	464.08711	463.08829	2.6	―	―	463[M − H]−;	6-Hydroxy-luteolin-7-O-glucoside	e
								301[M − H − Glc]−;
								273[M − H − Glc − CO]−;
								257[M − H − Glc − CO2]−;
								167[C7H3O5]−
29	20.93	C22H20O12	476.0871	475.08633	−1.6	―	―	475[M − H]−;	Kaempferol-3-O-β-d-glucuronic acid methyl ester	c
								457[M − H − H2O]−;
								433[M − H − CO=CH2]−;
								415[M − H − CH3COOH]−;
								285[M − H − C7H10O6]−
30	21.62	C15H10O7	302.03428	301.03396	−1.1	―	―	301[M − H]−;	6-Hydroxy-luteolin	e
								273[M − H − CO]−;
								257[M − H − CO2]−;
								167[C7H3O5]−
31	21,78	C26H28O16	596.14463	595.14543	1.4	―	―	595[M − H]−;	Quercetin-3-O-β-d-galactosyl-(1→2)-β-d-apioside	b
								505[M − H – Api + C2H2O]−;
								463[M − H − Api]−;
								445[M − H − Api − H2O]−;
								301[M − H − Api − Gal]−;
								300[M − H − Api − Gal − H]−;
								273[M − H − Api − Gal − CO]−;
								257[M − H − Api − Gal − 2CO]−;
								179[C8H3O5]−
32	21.88	C14H6O8	302.01355	―	―	303.01321	−1.1	303[M+H]+;	Ellagic acid	c
								285[M + H − H2O]+;
								275[M + H − CO]+;
								257[M + H − CO − H2O]+;
								247[M + H − 2CO]+;
								201[C11H5O4]+
33	22.26	C29H28O9	520.18061	―	―	521.18042	−0.4	521[M + H]+;	Schisantherin D	d
								399[M + H − C6H5COOH]+;
								355[M + H − C6H5COOH − C2H4O]+
34	22.9	C21H20O12	464.08711	463.08887	3.8	―	―	463[M − H]−;	Hyperoside	b,c
								301[M − H − Gal]−;
								255[M − H − Gal − CO − H2O]−;
								151[C7H3O4]−;
								107[C6H3O2]−
35	23.02	C16H12O6	300.05502	299.05453	−1.6	―	―	299[M − H]−;	Hispidulin	e
								271[M − H − CO]−;
								255[M − H − CO2]−;
								227[M − H − CO − CO2]−;
								181[C8H5O5]−
36	23.1	C27H30O16	610.14501	609.1479	4.7	―	―	609[M − H]−;	Rutin	c
								343[C17H11O8]−;
								301[M − H − Rha − Glc]−;
								300[M − H − Rha − Glc − H]−;
								271[M − H − Rha − Glc − H − CO − H]−;
								255[M − H − Rha − Glc − H − CO − OH]−;
								179[C8H3O5]−;
								151[C7H3O4]−
37	23.74	C21H20O12	464.08711	463.0874	0.6	―	―	463[M − H]−;	Isoquercitrin	c
								301[M − H − Glc]−;
								255[M − H − Glc − CO − H2O]−;
								151[C7H3O4]−;
								107[C6H3O2]−
38	24.2	C29H36O16	640.19197	639.19263	1	―	―	639[M − H]−;	Plantamajoside	e
								477[M − H − C9H6O3]−;
								315[M − H − C9H6O3 − Glc]−;
								153[M − H − C9H6O3 − 2Glc]−;
								135[M − H − C9H6O3 − 2Glc − H2O]−
39	24.95	C27H30O15	594.15009	593.1507	1	―	―	593[M − H]−;	Kaempferol-3-O-rutinoside	c
								327[M − H − C10H18O8]−;
								285[M − H − Rha − Glc]−;
								284[M − H − Rha − Glc − H]−;
								257[M − H − Rha − Glc − CO]−;
								255[M − H − Rha − Glc − H − CO − H]−;
								229[M − H − Rha − Glc − 2CO]−;
								227[M − H − Rha − Glc − H − 2CO − H]−
40	25.34	C28H36O8	500.24829	―	―	501.25002	3.4	501[M + H]+;	Tigloylgomisin H/Angeloygomi-sin H	d
								484[M + H − OH]+;
								483[M + H − H2O]+;
								384[M + H − OH − C4H7COOH]+;
								357[M + H − C4H7COOH − C2H4O]+
41	25.95	C21H20O11	448.09219	447.09378	3.6	―	―	447[M − H]−;	Luteoloside	e
								285[M − H − Glc]−;
								284[M − H − Glc − H]−;
								257[M − H − Glc − CO]−;
								243[M − H − Glc − C2H2O]−;
								241[M − H − Glc − CO2]−;
								199[M − H − Glc − C2H2O − CO2]−;
								197[M − H − Glc − 2CO2]−;
								151[C7H3O4]−
42	26.1	C28H36O8	500.24829	―	―	501.25071	4.8	501[M + H]+;	Tigloylgomisin H/Angeloygomi-sin H	d
								484[M + H − OH]+;
								483[M + H − H2O]+;
								384[M + H − OH − C4H7COOH]+;
								357[M + H − C4H7COOH − C2H4O]+
43	26.57	C29H36O15	624.19707	623.19604	−1.6	―	―	623[M − H]−;	Acteoside	e
								461[M − H − C9H6O3]−;
								315[M − H − C9H6O3 − Rha]−;
								179[C9H7O4]−;
								153[M − H − C9H6O3 − Rha − Glc]−;
								135[M − H − C9H6O3 − Rha − Glc − H2O]−
44	27.19	C21H20O11	448.09219	447.09225	0.1	449.10532	−2.5	447[M − H]−;	Astragalin	b,c
								285[M − H − Glc]−;
								151[C7H3O4]−;
								449[M + H]+;
								287[M − H − Glc]+;
								241[M − H − Glc − CH2O2]+;
								213[M + H − Glc − C2H2O3]+
45	27.19	C21H20O11	448.09219	447.09225	0.1	449.10699	−1.9	447[M − H]−;	Plantaginin	e
								285[M − H − Glc]−;
								267[M − H − Glc − H2O]−;
								255[M − H − Glc − CH2O]−;
								239[M − H − Glc − H2O − CO]−;
								167[C7H3O5]−;
								449[M + H]+;
								287[M+H − Glc]+;
								269[M + H − Glc − H2O]+;
								169[C7H5O5]+
46	27.48	C29H36O15	624.19705	623.19794	1.4	625.21005	−4.2	623[M − H]−;	Methylhesperidin	c
								477[M − H − Rha]−;
								315[M − H − Rha − Glc]−;
								300[M − H − Rha − Glc − CH3]−;
								297[M − H − Rha − Gldc − H2O]−;
								285[M − H − Rha − Glc − 2CH3]−;
								282[M − H − Rha − Glc − H2O − CH3]−;
								272[M − H − Rha − Glc − CH3 − CO]−;
								257[M − H − Rha − Glc − 2CH3 − CO]−;
								229[M − H − Rha − Glc − 2CH3 − 2CO]−;
								625[M + H]+;
								479[M + H − Rha]+;
								317[M + H − Rha − Glc]+;
								299[M + H − Rha − Glc − H2O]+;
								281[M + H − Rha − Glc − 2H2O]+;
								193[C10H9O4]+;
								165[C9H9O3]+
47	27.72	C21H20O11	448.09219	447.09402	4.1	―	―	447[M − H]−;	Quercitrin	c
								301[M − H − Rha]−;
								300[M − H − Rha − H]−;
								273[M − H − Rha − CO]−;
								255[M − H − Rha − CO − H2O]−
48	27.86	C22H22O12	478.10275	477.10461	3.9	―	―	477[M − H]−;	Nepetin-7-O-glucoside	e
								315[M − H − Glc]−;
								287[M − H − Glc − CO]−;
								271[M − H − Glc − CO2]−;
								181[C8H5O5]−
49	27.93	C21H20O10	432.09727	431.09805	1.8	433.11093	−4.6	431[M − H]−;	Kaempferol-7-O-α-l-rhamnoside	c
								413[M − H − H2O]−;
								285[M − H − Rha]−;
								267[M − H − Rha − H2O]−;
								169[C12H9O]−;
								155[C11H7O]−;
								433[M + H]+
50	27.97	C21H20O10	432.09727	431.09866	3.2			431[M − H]−;	Apigenin-7-O-glucoside	e
								269[M − H − Glc]−;
								201[M − H − Glc − C3O2]−;
								151[C7H3O4]−
51	27.99	C15H16O9	340.07106	339.07058	−1.4	―	―	339[M − H]−;	Esculin	c
								177[M − H − Glc]−;
								133[M − H − Glc − CO2]−;
								105[M − H − Glc − CO2 − CO]−;
								89[C7H5]−
52	28.28	C27H30O14	578.15518	577.15662	2.5	579.17073	−0.2	577[M − H]−;	Rhoifolin	e
								457[M − H − C4H8O4]−;
								431[M − H − Rha]−;
								413[M − H − Rha − H2O]−;
								269[M − H − Rha − Glc]−;
								225[M − H − Rha − Glc − CO2]−;
								183[C12H7O2]−;
								579[M + H]+;
								433[M + H − Rha]+;
								415[M + H − Rha − H2O]+;
								397[M + H − Rha − 2H2O]+;
								271[M + H − Rha − Glc]+;
								253[M + H − Rha − Glc − H2O]+;
								243[M + H − Rha − Glc − CO]+;
								225[M + H − Rha − Glc − H2O − CO]+;
								211[M + H − Rha − Glc − H2O − C2H2O]+;
								153[C7H5O4]+;
								119[C8H7O]+;
								91[C7H7]+
53	28.28	C28H32O16	624.16066	623.16248	2.9	625.17896	4.2	623[M − H]−;	Isorhamnetin-3-O-β-d-rutinoside	a
								477[M − H − Rha]−;
								315[M − H − Rha − Glc]−;
								300[M − H − Rha − Glc − CH3]−;
								272[M − H − Rha − Glc − CH3 − CO]−;
								271[M − H − Rha − Glc − CH3 − CO − H]−;
								243[M − H − Rha − Glc − CH3 − CO − H − CO]−;
								227[M − H − Rha − Glc − CH3 − CO − H − CO2]−;
								151[C7H3O4]−;
								625[M + H]+;
								479[M + H − Rha]+;
								317[M + H − Rha − Glc]+;
								302[M + H − Rha − Glc − CH3]+;
								285[M + H − Rha − Glc − CH3OH]+;
								274[M + H − Rha − Glc − CH3 − CO]+;
								257[M + H − Rha − Glc − CH3OH − CO]+;
								246[M + H − Rha − Glc − CH3 − 2CO]+;
								229[M + H − Rha − Glc − CH3OH − 2CO]+;
								153[C7H5O4]+
54	28.31	C21H18O11	446.07654	445.07493	−3.6	―	―	445[M − H]−;	Baicalin	e
								269[M − H − GluA]−;
								225[M − H − GluA − CO2]−;
								167[C7H3O5]−
55	29.09	C20H26O4	330.19039	―	―	331.18914	−3.8	331[M + H]+;	Meso-dihydrog-uaiaretic acid	d
								313[M + H − H2O]+;
								301[M + H − 2CH3]+
56	29.58	C15H10O5	270.0601	269.09566	−1.6	271.05966	−1.7	269[M − H]−;	Baicalein	e
								241[M − H − CO]−;
								225[M − H − CO2]−;
								197[M − H − CO − CO2]−;
								167[C7H3O5]−;
								271[M + H]+;
								253[M + H − H2O]+;
								169[C7H5O5]+
57	30.7	C28H34O15	610.1814	609.18229	1.5	611.1994	3.9	609[M − H]−;	Neohesperidin	c
								505[M − H − C4H8O3]−;
								463[M − H − Rha]−;
								445[M − H − Rha − H2O]−;
								427[M − H − Rha − 2H2O]−;
								301[M − H − Rha − Glc]−;
								286[M − H − Rha − Glc − CH3]−;
								283[M − H − Rha − Glc − H2O]−;
								268[M − H − Rha − Glc − H2O − CH3]−;
								258[M − H − Rha − Glc − CH3 − CO]−;
								257[M − H − Rha − Glc − CO2]−;
								242[M − H − Rha − Glc − CH3 − CO2]−;
								239[M − H − Rha − Glc − H2O − CO2]−;
								199[C12H7O3]−;
								125[C6H5O3]−;
								611[M + H]+;
								303[M + H − Rha − Glc]+;
								285[M + H − Rha − Glc − H2O]+;
								179[C9H7O4]+;
								177[C10H9O3]+;
								151[C8H7O3]+
58	31.48	C23H28O8	432.1857	―	―	433.18538	−0.7	433[M + H]+;	Schizandradiol	d
								415[M + H − H2O]+;
								361[M + H − C4H8O]+;
								372[M + H − H2O − CH3CO]+;
								343[M + H − H2O − C4H7OH]+
59	31.54 39.44	C15H10O5	270.04445	269.04353	−3.4	271.06145	4.9	269[M − H]−;	Apigenin	e
								241[M − H − CO]−;
								227[M − H − C2H2O]−;
								225[M − H − CO2]−;
								201[M − H − C3O2]−;
								151[C7H3O4]−;
								271[M + H]+;
								253[M + H − H2O]+;
								225[M + H − H2O − CO]+
60	31.71	C28H34O15	610.1814	609.18351	3.5	611.20001	4.8	609[M − H]−;	Hesperidin	c
								463[M − H − Rha]−;
								301[M − H − Rha − Glc]−;
								286[M − H − Rha − Glc − CH3]−;
								283[M − H − Rha − Glc − H2O]−;
								268[M − H − Rha − Glc − H2O − CH3]−;
								258[M − H − Rha − Glc − CH3 − CO]−;
								257[M − H − Rha − Glc − CO2]−;
								242[M − H − Rha − Glc − CH3 − CO2]−;
								239[M − H − Rha − Glc − H2O − CO2]−;
								199[C12H7O3]−;
								125[C6H5O3]−;
								611[M + H]+;
								303[M + H − Rha − Glc]+;
								285[M + H − Rha − Glc − H2O]+;
								179[C9H7O4]+;
								177[C10H9O3]+;
								151[C8H7O3]+
61	32.13	C15H10O7	302.03428	301.03549	4	303.0488	−1.1	301[M − H]−;	Quercetin	b,c
								273[M − H − CO]−;
								255[M − H − CO − H2O]−;
								151[C7H3O4]−;
								303[M + H]+;
								285[M + H − H2O]+;
								257[M + H − H2O − CO]+;
								247[M + H − 2CO]+;
								229[M + H − H2O − 2CO]+
62	33.02	C30H26O13	594.12897	593.13049	2.6	595.14142	−3.2	593[M − H]−;	Tiliroside	c
								447[M − H − C9H6O2]−;
								285[M − H − C9H6O2 − Glc]−;
								257[M − H − C9H6O2 − Glc − CO]−;
								229[M − H − C9H6O2 − Glc − 2CO]−;
								151[C7H3O4]−;
								617[M + Na]+;
								595[M + H]+;
								449[M + H − C9H6O2]+;
								287[M + H − C9H6O2 − Glc]+
63	34.12	C15H12O6	288.05502	287.05549	1.7	289.07002	−2.2	287[M − H]−;	Aromadendrin	c
								269[M − H − H2O]−;
								259[M − H − CO]−;
								243[M − H − CO2]−;
								225[M − H − H2O − CO2]−;
								215[M − H − CO − CO2]−;
								151[C7H3O4]−;
								107[C6H3O2]−;
								289[M + H]+;
								271[M + H − H2O]+;
								195[C9H7O5]+;
								145[C9H5O2]+
64	35.18	C15H10O6	286.03936	285.03995	2.1	287.05359	4.9	285[M − H]−;	Kaempferol	b,c
								257[M − H − CO]−;
								229[M − H − 2CO]−;
								151[C7H3O4]−;
								309[M + Na]+;
								287[M + H]+;
								241[M + H − CH2O2]+;
								213[M + H − C2H2O3]+
65	35.31	C15H10O6	286.03936	285.04019	2.9	―	―	571[2M − H]−;	Iuteolin	e
								285[M − H]−;
								243[M − H − C2H2O]−;
								241[M − H − CO2]−;
								199[M − H − C2H2O − CO2]−;
								197[M − H − 2CO2]−;
								171[M − H − C2H2O − CO2 − CO]−;
								151[C7H3O4]−
66	35.58	C16H12O7	316.04993	315.05117	3.9	―	―	315[M − H]−;	Nepetin	e
								287[M − H − CO]−;
								271[M − H − CO2]−;
								243[M − H − CO − CO2]−;
								181[C8H5O5]−
67	35.61	C15H10O6	286.03937	285.03989	1.8	287.05627	4.4	285[M − H]−;	Scutellarein	e
								257[M − H − CO]−;
								241[M − H − CO2]−;
								167[C7H3O5]−;
								287[M + H]+;
								269[M + H − H2O]+;
								241[M + H − H2O − CO]+;
								169[C7H5O5]+
68	35.61	C16H12O7	316.04993	315.05027	1.1	317.06486	−2.3	315[M − H]−;	Isorhamnetin	b
								300[M − H − CH3]−;
								272[M − H − CH3 − CO]−;
								271[M − H − CH3 − CO − H]−;
								243[M − H − CH3 − CO − H − CO]−;
								227[M − H − CH3 − CO − H − CO2]−;
								151[C7H3O4]−;
								107[C6H3O2]−;
								339[M + Na]+;
								317[M + H]+;
								302[M + H − CH3]+;
								285[M + H − CH3OH]+;
								274[M + H − CH3 − CO]+;
								257[M + H − CH3OH − CO]+;
								246[M + H − CH3 − 2CO]+;
								229[M + H − CH3OH − 2CO]+;
								153[C7H5O4]+
69	35.76	C30H46O4	470.34689	469.33251	2.7	471.34509	−3.8	469[M − H]−;	Nigranoic acid	d
								423[M − H − HCOOH]−;
								378[M − H − HCOOH − HCOO]−;
								471[M + H]+;
								456[M + H − CH3]+;
								453[M + H − H2O]+;
								390[M + H − C6H9]+
70	35.89	C28H24O16	616.09807	615.10097	4.7	―	―	615[M − H]−;	2″-O-Galloylhyperoside	c
								463[M − H − C7H4O4]−;
								445[M − H − C7H6O5]−;
								301[M − H − C7H4O4 − Glc]−
71	36.79	C23H30O7	418.20643	―	―	419.20477	−4.0	419[M + H]+;	Gomisin H	d
								401[M + H − H2O]+;
								384[M + H − OH − H2O]+;
								383[M + H − 2H2O]+;
								369[M + H − OH − CH3 − H2O]+;
								353[M + H − OH − OCH3 − H2O]+
72	37.08	C28H34O8	498.23264	―	―	499.23119	−2.9	499[M + H]+;	Angeloygomisin O/Angeloylisogomisin O	d
								399[M + H − C4H7COOH]+;
								369[M + H − C4H7COOH − CH2O]+;
								357[M + H − C4H7COOH − C3H6]+;
								343[M + H − C4H7COOH − C4H8]+
73	38.12	C28H34O8	498.23264	―	―	499.23026	−4.8	499[M + H]+;	Angeloygomisin O/Angeloylisogomisin O	d
								399[M + H − C4H7COOH]+;
								369[M + H − C4H7COOH − CH2O]+;
								357[M + H − C4H7COOH − C3H6]+;
								343[M + H − C4H7COOH − C4H8]+
74	39.28	C23H28O7	416.19078	―	―	417.18903	−4.2	417[M + H]+;	Gomisin O/Epigomisin O	d
								402[M + H − CH3]+;
								399[M + H − H2O]+;
								385[M + H − CH3OH]+;
								373[M + H − C2H4O]+
75	39.38	C24H32O7	432.22209	―	―	433.22103	−2.4	433[M + H]+;	Schisandrin	d
								415[M + H − H2O]+;
								400[M + H − H2O − CH3]+;
								384[M + H − H2O − OCH3]+;
								373[M + H − H2O − C3H6]+;
								359[M + H − H2O − C4H8]+
76	39.44	C30H46O4	470.34689	―	―	471.34551	−2.9	471[M + H]+;	Kadsuric acid	d
								453[M + H − H2O]+;
								398[M + H − CH2CH2COOH]+;
								370[M + H − CH2CH2COOH − C2H4]+
77	39.6	C23H28O7	416.19078	―	―	417.19103	0.6	417[M + H]+;	Gomisin O/Epigomisin O	d
								402[M + H − CH3]+;
								399[M + H − H2O]+;
								385[M + H − CH3OH]+;
								373[M + H − C2H4O]+
78	40.63	C28H34O10	530.22247	―	―	531.22218	−0.6	531[M + H]+;	Gomisin D	d
								485[M + H − CH2O2]+;
								401[M + H − C6H10O3]+;
								383[M + H − C6H10O3 − H2O]+
79	40.98	C22H28O6	388.19586	―	―	389.19503	−2.1	389[M + H]+;	Gomisin J	d
								374[M + H − CH3]+;
								357[M + H − CH3OH]+;
								342[M + H − CH3OH − CH3]+
80	41.02	C30H48O5	488.3418	487.3439	4.3	489.35526	−4.5	487[M − H]−;	Rosolic acid/2α,3α,19α-Trihydroxyolean-12-ene-28-oic acid/Arjunolic acid	c
								469[M − H − H2O]−;
								443[M − H − CO2]−;
								425[M − H − CO2 − H2O]−;
								407[M − H − CO2 − 2H2O]−;
								391[M − H − CO2 − 2H2O − CH4]−;
								489[M + H]+;
								453[M + H − 2H2O]+
81	41.22	C30H48O5	488.3418	487.34378	4	489.35544	−4.1	487[M − H]−;	Rosolic acid/2α,3α,19α-Trihydroxyolean-12-ene-28-oic acid/Arjunolic acid	c
								469[M − H − H2O]−;
								443[M − H − CO2]−;
								425[M − H − CO2 − H2O]−;
								407[M − H − CO2 − 2H2O]−;
								391[M − H − CO2 − 2H2O − CH4]−;
								489[M + H]+;
								453[M + H − 2H2O]+
82	41.75	C15H24N2O2	264.10889	―	―	265.18997	−4.0	265[M + H]+;	Sophoranol	b
								248[M + H − OH]+;
								247[M + H − H2O]+
83	42.18	C30H46O3	454.35198	―	―	455.35025	−3.8	455[M + H]+;	Ganwuweizic acid	d
								409[M + H − HCOOH]+;
								313[M + H − C8H14O2]+
84	42.32	C30H48O5	488.3418	487.34302	2.5	489.35529	−4.4	487[M − H]−;	Rosolic acid/2α,3α,19α-Trihydroxyolean-12-ene-28-oic acid/Arjunolic acid	c
								469[M − H − H2O]−;
								443[M − H − CO2]−;
								425[M − H − CO2 − H2O]−;
								407[M − H − CO2 − 2H2O]−;
								391[M − H − CO2 − 2H2O − CH4]−;
								489[M + H]+;
								453[M + H − 2H2O]+
85	42.89	C12H16N2O	204.13354	―	―	205.13409	2.6	205[M + H]+;	N-methylcytisine	b
								146[M + H − C3H9N]+;
								108[C6H6NO]+
86	43.27	C23H28O6	400.19587	―	―	401.19434	−3.8	401[M + H]+;	Schizandrin B	d
								386[M + H − CH3]+;
								370[M + H − OCH3]+;
								355[M + H − CH3 − OCH3]+;
								339[M + H − 2CH3 − CH3OH]+
87	43.3	C30H34O8	522.23265	―	―	523.23095	−3.2	523[M + H]+;	Benzoylgomisin H	d
								505[M + H − H2O]+;
								401[M + H − C6H5COOH]+;
								383[M + H − C6H5COOH − H2O]+
88	45.42	C23H30O6	402.21151	―	―	403.21011	−3.5	403[M + H]+;	Schisanhenol	d
								372[M + H − OCH3]+;
								371[M + H − CH3OH]+;
								356[M + H − CH3OH − CH3]+;
								340[M + H − OCH3 − CH3OH]+
89	45.44	C23H28O7	416.19078	―	―	417.18957	−2.9	417[M + H]+;	Schisandrol B	d
								399[M + H − H2O]+;
								343[M + H − C4H8 − H2O]+;
								307[M + H − 2CH3 − 2OCH3 − H2O]+
90	45.67	C28H34O9	514.22755	―	―	515.22559	−3.8	515[M + H]+;	Schisantherin B/Schisantherin C	d
								415[M + H − C4H7COOH]+;
								385[M + H − C4H7COOH − CH2O]+;
								367[M + H − C4H7COOH − CH2O − H2O]+;
								353[M + H − C4H7COOH − CH2O − CH3OH]+
91	46.06	C30H32O9	536.21191	―	―	537.20981	−3.9	537[M + H]+;	Schisantherin A	d
								415[M + H − C6H5COOH]+;
								397[M + H − C6H5COOH − H2O]+;
								373[M + H − C6H5COOH − C3H6]+
92	46.58	C30H48O4	472.34689	471.34872	3.9	473.30911	−3.7	471[M − H]−;	Corosolic acid/Maslinic acid	c
								453[M − H − H2O]−;
								427[M − H − CO2]−;
								391[M − H − CO2 − 2H2O]−;
								473[M + H]+;
								455[M + H − H2O]+;
								203[C15H23]+;
								105[C8H9]+
93	46.73	C15H24N2O	248.19614	―	―	249.19699	2.2	271[M + Na]+;	Matrine	b
								249[M+H]+;
								231[M + H − H2O]+;
								150[C10H16N]+;
								148[C10H14N]+
94	47.28	C22H26O6	386.18022	―	―	387.17992	−0.8	387[M + H]+;	Gomisin L1/Gomisin L2/Gomisin M1/Gomisin M2	d
								372[M + H − CH3]+;
								369[M + H − H2O]+;
								357[M + H − CH2O]+;
								329[M + H − C2H2O2]+
95	47.52	C30H48O4	472.34689	471.34863	3.7	473.36044	−4.4	471[M − H]−;	Corosolic acid/Maslinic acid	c
								453[M − H − H2O]−;
								427[M − H − CO2]−;
								391[M − H − CO2 − 2H2O]−;
								473[M + H]+;
								455[M + H − H2O]+;
								203[C15H23]+;
								105[C8H9]+
96	47.53	C22H26O6	386.18022	―	―	387.17847	−4.5	387[M + H]+;	Gomisin L1/Gomisin L2/Gomisin M1/Gomisin M2	d
								372[M + H − CH3]+;
								369[M + H − H2O]+;
								357[M + H − CH2O]+;
								329[M + H − C2H2O2]+
97	47.76	C28H34O9	514.22755	―	―	515.22716	−0.7	515[M + H]+;	Schisantherin B/Schisantherin C	d
								415[M + H − C4H7COOH]+;
								385[M + H − C4H7COOH − CH2O]+;
								367[M + H − C4H7COOH − CH2O − H2O]+;
								353[M + H − C4H7COOH − CH2O − CH3OH]+
98	48.01	C30H48O3	456.36763	455.35424	4.9	457.36636	−2.7	455[M − H]−;	Oleanolic acid/Ursolic acid	c,e
								407[M − H − HCHO − H2O]−;
								391[M − H − HCHO − H2O − CH4]−;
								479[M + Na]+;
								457[M + H]+;
								439[M + H − H2O]+;
								393[M + H − HCOOH − H2O]+
99	48.24	C22H26O6	386.18022	―	―	387.17883	−3.6	387[M + H]+;	Gomisin L1/Gomisin L2/Gomisin M1/Gomisin M2	d
								372[M + H − CH3]+;
								369[M + H − H2O]+;
								357[M + H − CH2O]+;
								329[M + H − C2H2O2]+
100	48.4	C30H48O3	456.36763	455.35394	4.3	457.36612	−3.3	455[M − H]−;	Oleanolic acid/Ursolic acid	c,e
								407[M − H − HCHO − H2O]−;
								391[M − H − HCHO − H2O − CH4]−;
								479[M + Na]+;
								457[M + H]+;
								457[M + H − H2O]+;
								393[M + H − HCOOH − H2O]+
101	48.65	C22H26O6	386.18022	―	―	387.17877	−3.7	387[M + H]+;	Gomisin L1/Gomisin L2/Gomisin M1/Gomisin M2	d
								372[M + H − CH3]+;
								369[M + H − H2O]+;
								357[M + H − CH2O]+;
								329[M + H − C2H2O2]+
102	49.79	C24H32O6	416.22714	―	―	417.22649	−1.6	417[M + H]+;	Deoxyschizan-drin	d
								402[M + H − CH3]+;
								386[M + H − OCH3]+;
								370[M + H − CH3 − CH3OH]+;
								347[M + H − CH3 − C4H7]+;
								316[M + H − C6H13O]+;
								286[M + H − C6H13O − 2CH3]+;
								273[M + H − C6H13O − CH3 − CO]+;
								227[M + H − C6H13O − CO − OCH3 − 2CH3]+
103	52.02	C16H14O4	270.09649	―	―	271.09595	−2.0	271[M + H]+; 203[M + H − C5H8]+;	Imperatorin	c
								175[M + H − C5H8 − CO]+;
								159[M + H − C5H8 − CO2]+
104	53.29	C30H32O8	520.21701	―	―	521.21559	−2.7	521[M + H]+;	Benzoylgomisin O/Benzoyisolgomisin O	d
								399[M + H − C6H5COOH]+;
								369[M + H − C6H5COOH − CH2O]+;
								357[M + H − C6H5COOH − C3H6]+;
								343 M + H − C6H5COOH − C4H8]+
105	53.43	C22H24O6	384.16457	―	―	385.16406	−1.3	385[M + H]+;	Schizandrin C	d
								370[M + H − CH3]+;
								355[M + H − CH2O]+
106	54.08	C30H32O8	520.21701	―	―	521.21611	−1.7	521[M + H]+;	Benzoylgomisin O/Benzoyisolgomisin O	d
								399[M + H − C6H5COOH]+;
								369[M + H − C6H5COOH − CH2O]+;
								357[M + H − C6H5COOH − C3H6]+;
								343 M + H − C6H5COOH − C4H8]+

a: Fructus Lych, b: Semen Cuscutae (fried), c: Fructus Rubi, d: Fructus Schisandrae chinensis (steamed), e: Semen Plantaginis (fried with salt); Glc : β-d-glucose, GluA: Glucuronic acid, Xyl: β-d-xylose, Rha: l-rhamnose; Gal: d-galactose; Api: d-apiose, ppm: difference between calculated and found mass.

2.2.1. Flavonoids

Flavonoids have a diphenylpropane skeleton bearing two benzene rings (A and B) connected by a pyran ring attached to the A ring, and are further divided into several subclasses (flavones, flavonols, flavanones, flavanonols, anthocyanidins, aurone, halcones and isoflavonoids). In this work, four types of flavonoids were found in WZYZW by UPLC–ESI-LTQ-Orbitrap-MS. Taking compound 53 as an example, the precise molecular weight is 623.16248 (C₂₈H₃₁O₁₆) and in the negative ion spectrum, the main fragment ions were observed at m/z 623 [M − H]⁻, 477 [M − H − Rha]⁻, 315 [M − H − Rha − Glc]⁻, 300 [M − H − Rha − Glc − CH₃]⁻, 272 [M − H − Rha − Glc − CH₃ − CO]⁻, 271 [M − H − Rha − Glc − CH₃ − CO − H]⁻, 243 [M − H − Rha − Glc − CH₃ − CO − H − CO]⁻, 227 [M − H − Rha − Glc − CH₃ − CO − H − CO₂]⁻, and 151 [C₇H₃O₄]⁻, thus, compound 53 was identified as isorhamnetin-3-O-β-d-rutinoside. Its mass spectrum and proposed fragmentation pathways in negative mode are shown in Figure 2.

Figure 2. ESI-CID-MS³ spectra and proposed fragmentations for isorhamnetin-3-O-β-d-rutinoside (53) from WZYZW.

Figure 2. ESI-CID-MS³ spectra and proposed fragmentations for isorhamnetin-3-O-β-d-rutinoside (53) from WZYZW.

Compounds 34 and 37 were considered to be isomers as they displayed the same [M − H]⁻ ions at m/z 463.08887 (C₂₁H₁₉O₁₂) and 463.08740 (C₂₁H₁₉O₁₂). Moreover, they had the similar fragment ions of m/z 301, 255 and 151 in the MSⁿ spectra, but different max UV absorption wavelengths (λ_max), as the λ_max of compound 34 were 254 and 356 nm, and compound 37 showed λ_max peaks at 254 and 346 nm. Considering the different retention times, compounds 34 and 37 were tentatively identified as hyperoside and isoquercitrin, respectively (see Table 1).

Similarly, based on the chromatographic behavior and MSⁿ spectrometry for further confirming the fragmentation patterns as shown above, a total of 35 flavonoids were tentatively identified, including 15 flavones and derivatives, 16 flavonols and derivatives, three flavanone glycosides, and one flavanonol, which are summarized in Figure 3.

Figure 3. Flavonoids identified in WZYZW by UPLC-ESI-LTQ-Orbitrap-MS.

Figure 3. Flavonoids identified in WZYZW by UPLC-ESI-LTQ-Orbitrap-MS.

2.2.2. Phenylpropanoids

Phenylpropanoids have one or more C₆-C₃ structures and there are three main subclasses: simple phenylpropanoids, coumarins and lignans. In the present study, 34 phenylpropanoids were found in WZYZW, including two simple phenylpropanoids, four coumarins and 28 lignans. The classification of these compounds is shown in Figure 4.

Figure 4. Phenylpropanoids identified in WZYZW by UPLC-ESI-LTQ-Orbitrap-MS.

Figure 4. Phenylpropanoids identified in WZYZW by UPLC-ESI-LTQ-Orbitrap-MS.

Compound 102 afforded a quasi-molecular ion at m/z 417.22649 (C₂₄H₃₃O₆) in positive mode, and its MSⁿ spectra showed representative ions at m/z 417 [M + H]⁺, 402 [M + H − CH₃]⁺, 386 [M + H − OCH₃]^+., 370 [M + H − CH₃ − CH₃OH]⁺, 347 [M + H − CH₃ − C₄H₇]⁺, 316 [M + H − C₆H₁₃O]⁺, 286 [M + H − C₆H₁₃O − 2CH₃]⁺, 273 [M + H − C₆H₁₃O − CH₃ − CO]⁺, and 227 [M + H − C₆H₁₃O – CO − OCH₃ − 2CH₃]⁺. Consequently, compound 102 was tentatively identified as deoxyschizandrin. The MSⁿ mass spectra and the fragmentation pathways of deoxyschizandrin are shown in Figure 5. As with the fragmentations described above, the other phenylpropanoid compounds were also tentatively identified. However, six groups of isomers (compounds 40 and 42, 72 and 73, 74 and 77, 90 and 97, 94, 96, 99 and 101, 104 and 106) had the same molecular weights in the MS spectra, similar fragment ions in the MSⁿ spectra and similar max UV absorption wavelengths. Thus we could not distinguish them, and these structures will require further confirmation (see Table 1).

Figure 5. ESI-CID-MS³ spectra and proposed fragmentations for deoxyschizandrin (102) from WZYZW.

Figure 5. ESI-CID-MS³ spectra and proposed fragmentations for deoxyschizandrin (102) from WZYZW.

2.2.3. Organic Acids

The UPLC-ESI-LTQ-Orbitrap method was applied to comprehensively characterize the organic acids in this study, and the results showed that three types of organic acids could be detected in WZYZW: four aliphatic organic acids, three alicyclic organic acids and 10 phenolic acids. The specific details are displayed in Figure 6.

Figure 6. Organic acids identified in WZYZW by UPLC-ESI-LTQ-Orbitrap-MS.

Figure 6. Organic acids identified in WZYZW by UPLC-ESI-LTQ-Orbitrap-MS.

Compound 15 produced a [M − H]⁻ ion at m/z 167.03420 (C₈H₇O₄), and its fragment ions showed the losses of CH₃, CO₂ and CH₃ + CO₂, respectively. Thus, compound 15 was identified as vanillic acid, which was confirmed by MSⁿ experiments. Similarly, the above fragmentation patterns were applied to further confirm the identities of the other organic acids compound, and finally, 17 compounds were identified as organic acids.

2.2.4. Terpenoids

As displayed in Figure 7, the precise molecular weight of compound 16 is 373.11377 (C₁₆H₂₁O₁₀) in the negative ion spectrum, and the main fragment ions were observed at m/z 373 [M − H]⁻, 329 [M – H − CO₂]⁻, 211 [M − H − Glc]⁻, 193 [M − H − Glc − H₂O]⁻, 167 [M − H − Glc − CO₂]⁻ and 149 [M − H − Glc − CO₂ − H₂O]⁻. Therefore, compound 16 was tentatively identified as geniposidic acid.

Using high-resolution MSⁿ mass spectrometry and the similar method of analysis of the aforementioned fragmentations, a total of 11 terpenoids were found: one iridoid (compound 16) was identified as geniposidic acid; 10 triterpenes—compounds 69, 76, 80, 81, 83, 84, 92, 95, 98 and 100 were identified as nigranoic acid, kadsuric acid, rosolic acid, 2α,3α,19α-trihydroxyolean-12-ene-28-oic acid, ganwuweizic acid, arjunolic acid, corosolic acid, maslinic acid, oleanolic acid and ursolic acid. Compounds 80, 81 and 84, 98 and 100, and 92 and 95, constituted three groups of isomers with similar fragmentation pathways in their MS spectra, so we could not precisely identify them by Mass (see Table 1).

2.2.5. Alkaloids

Compound 5 exhibited a [M + H]⁺ ion and a [M − H]⁻ at m/z 124.03876 (C₆H₆NO₂) and 122.02417 (C₆H₄NO₂), respectively, and positive product ions at m/z 124 [M + H]⁺ and 106 [M + H − H₂O]⁺ were detected. Thus, compound 5 was inferred as nicotinic acid (see Table 1). Similarly, eight alkaloids were identified in WZYZW and confirmed using MSⁿ data: abromine (1), nicotinic acid (5), thiamine (6), taurine (14), atropine (19), sophoranol (82), n-methylcytisine (85), and matrine (93).

2.2.6. Miscellaneous

Riboflavin (compound 10) was also identified in WZYZW by its [M − H]⁻ ion at m/z 375.12988 (C₁₇H₁₉N₄O₆). The corresponding MSⁿ spectra showed a peak at m/z 255 [C₁₃H₁₁N₄O₂]⁻, and another fragment ion was also observed at m/z 212 [C₁₂H₁₀ N₃O]⁻.

To summarize, in this study, a reliable and rapid UPLC-ESI-LTQ-Orbitrap-MS method has been established for the first comprehensive analysis of the phytochemical constituents of the Chinese herbal prescription WZYZW. This method revealed that UPLC-ESI-LTQ-Orbitrap-MS was useful for screening and identifying the complex constituents of WZYZW. Based on the method, a total of 106 compounds were tentatively characterized. In contrast to the published papers, it is noteworthy that this work detected more components. Our results provide essential data for further pharmacological studies and clinical evaluation of WZYZW and be useful for quality control of WZYZW, so as to guarantee its safe use in the clinic.

Figure 7. ESI-CID-MS³ spectra and proposed fragmentations for geniposidic acid (16) from WZYZW.

Figure 7. ESI-CID-MS³ spectra and proposed fragmentations for geniposidic acid (16) from WZYZW.

3. Experimental Section

3.1. Materials and Reagents

Fructus Lych, Semen Cuscutae (fried), Fructus Rubi, Fructus Schisandrae chinensis (steamed), and Semen Plantaginis (fried with salt) were supplied by the Tongrentang Drug Store in Beijing (China). The origins of the crude drugs were identified by Professor Rui Chao Lin of the Department of Pharmacognosy, Beijing University of Chinese Medicine. Voucher specimens were deposited at the authors’ laboratory. Reference standards of abromine, nicotinic acid, thiamine, riboflavin, taurine, quinic acid, atropine, ferulic acid, chlorogenic acid, scopoletin, rutin, esculin, apigenin, hesperidin, quercetin, kaempferol, luteolin, isorhamnetin, schisandrin were purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Acetonitrile and methanol (HPLC grade) were purchased from Fisher Scientific Co. (Waltham, MA, USA). Distilled water was purchased from Watson’s Food & Beverage Co. (Guangzhou, China). Formic acid, analytical grade, was obtained from Beijing Reagent Company (Beijing, China). Other reagents were HPLC grade or analytical reagent grade. High-purity nitrogen (99.9%) and helium (99.99%) were purchased from Gas Supplies Center of Peking University Health Science Center (Beijing, China).

3.2. Preparation of WZYZW Samples

A standard preparation of WZYZW was made in accordance with the 2015 version of the Chinese Pharmacopoeia. Fructus Lych, Semen Cuscutae (fried), Fructus Rubi, Fructus Schisandrae chinensis (steamed), and Semen Plantaginis (fried with salt) (8:8:4:2:1) were weighed, then crushed to powder (40 mesh size) and immersed in 10-fold volume of water for 1 h and heated. After boiling, heating was ontinued until the volume was reduced by 5-fold. The mixture was filtered through gauze while hot, concentrated to 1 g crude drug/mL, and freeze-dried to a powder. Finally refined honey (85 g) was mixed with freeze-dried powder (100 g) to make boluses of WZZYW for the experiments. Freeze-dried single herb extract powders were prepared as described for WZYZW. Freeze-dried samples of WZYZW (250 mg) were extracted with 50 mL of methanol with the aid of ultrasound for 60 min. The extracts were centrifuged at 10,000 rpm for 15 min at 4 °C, the supernatant was collected and filtered through a filter (0.22 μm), and the filtrate was collected for UPLC-LTQ-Orbitrap analysis. Respective standard stock solutions of 19 components were prepared at concentrations of 50 ng/mL by weighing the desired amount of each component into a volumetric flask and dissolving it in 100% ethanol; the 19 samples were filtered through a filter (0.22 μm), and the filtrates were analyzed by UPLC-LTQ-Orbitrap.

3.3. Instrumentation and Conditions

3.3.1. Liquid Chromatography Conditions

The chromatographic separation was performed on an ACQUITY UPLC^TM BEH C₁₈ column (1.7 μm, 2.1 mm × 100 mm) using an ACQUITY UPLC^TM system (Waters Corporation, Milford, MA, USA) equipped with quaternary pump, vacuum degasser, autosampler and photodiode array detector. A linear gradient elution of A (HCOOH:H₂O = 0.1:100) and B (HCOOH:CH₃CN = 0.1:100) was used. The optimized gradient program is shown in Table 2. The flow rate was 0.3 mL/min and column temperature was set at 30 °C, the injection volume was 3 μL. The effluent was roughly split at a ratio of 3:1 (v/v) before entering the ESI source.

3.3.2. ESI-MS/MS Detection

The LTQ/Orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany) was equipped with an ESI source operating in positive and negative ESI mode. The negative ion mode operation parameters were as follows: capillary voltage, 35 V; electrospray voltage, 3.0 kV; capillary temperature, 350 °C; sheath gas, 30 (arbitrary units); auxiliary gas, 10 (arbitrary units); tube lens, 110 V. The positive ion mode operation parameters: capillary voltage, 25 V; electrospray voltage, 4.0 kV; capillary temperature, 350 °C; sheath gas, 30 (arbitrary units); auxiliary gas, 5(arbitrary units ); tube lens, 110 V. Samples were detected by full-scan mass analysis from m/z 100 to 1000 at a resolving power of 30,000 with data-dependent MS² analysis triggered by the three most-abundant ions from the predicted precursor list followed by MS³ analysis of the most-abundant product ions. To avoid performing many repeated data acquisitions on the same sample, dynamic exclusion is used for the data collection with an exclusion duration of 60s and the repeat count was set at 5 with a dynamic repeat time at 30 s. Collision-induced dissociation (CID) was performed with an isolation width of 2 Da. The collision energy was set to 35%. An external calibration for mass accuracy was carried out before the analysis. The measured masses were within 5ppm of the theoretical masses. The data analysis was achieved using XCalibur softwarev2.0.7 (Thermo Fisher Scientific).

3.4. Data Processing

The data analysis was processed using Thermo Xcaliber 2.1 workstation (Thermo Fisher Scientific) for peak detection and peak alignment, the raw data were processed by the computer-based NMDF approach.

Table 2. Solvent gradient program of UPLC analysis.

Time (min)	Flow (mL/min)	A (%)	B (%)
0	0.300	99.0	1.0
1.0	0.300	99.0	1.0
12	0.300	91.0	9.0
17	0.300	88.0	12.0
17.5	0.300	86.5	13.5
23	0.300	86.5	13.5
33	0.300	70	30.0
35	0.300	60	40.0
50	0.300	35	65.0
52	0.300	35	65.0
55	0.300	1.0	99.0

Table 2. Solvent gradient program of UPLC analysis.

Time (min)	Flow (mL/min)	A (%)	B (%)
0	0.300	99.0	1.0
1.0	0.300	99.0	1.0
12	0.300	91.0	9.0
17	0.300	88.0	12.0
17.5	0.300	86.5	13.5
23	0.300	86.5	13.5
33	0.300	70	30.0
35	0.300	60	40.0
50	0.300	35	65.0
52	0.300	35	65.0
55	0.300	1.0	99.0

4. Conclusions

In this study, a rapid, sensitive and reliable UPLC-ESI-LTQ-Orbitrap-MS method was established for screening and identifying the chemical constituents of WZYZW in both the positive and negative ion modes. Based on the chromatographic and spectrometric data, and referring to the literature, we were able to tentatively characterize 109 compounds, including organic acids, flavonoids, phenylpropanoids, alkaloids and terpenoids. In all 14 ingredients from Fructus Lych were identified, 11 ingredients from Semen Cuscutae (fried), 35 ingredients from Fructus Rubi, 37 ingredients from Fructus Schisandrae chinensis (steamed), and 21 ingredients from Semen Plantaginis (fried with salt). Our results broadens the chemical knowledge of WZYZW, which should be helpful for the quality control of WZYZW, and for further research on the pharmacokinetic studies and the health and medical properties of WZYZW. Moreover, with the successful application of the UPLC-ESI-LTQ-Orbitrap-MS to characterizing the constituents of WZYZW, it is suggest that this method offer a rapid, sensitive and high throughput methodology for the identification of constituents of TCM prescriptions and herbal medicines.