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Molecules 2015, 20(11), 20659-20668; doi:10.3390/molecules201119729

Cyclovirobuxine D Inhibits Cell Proliferation and Induces Mitochondria-Mediated Apoptosis in Human Gastric Cancer Cells

1,2,* , 3
,
4
and
1,5
1
School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China
2
Ministry of Education of China (MOE) Key Laboratory of Hydrodynamics, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China
3
Department of General Surgery, School of Medicine, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University, Shanghai 200092, China
4
School of Pharmacy, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China
5
Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 17 August 2015 / Revised: 12 November 2015 / Accepted: 13 November 2015 / Published: 19 November 2015
(This article belongs to the Section Medicinal Chemistry)
View Full-Text   |   Download PDF [3166 KB, uploaded 19 November 2015]   |  

Abstract

Gastric cancer is one of the most common malignant cancers, with high death rates, poor prognosis and limited treatment methods. Cyclovirobuxine D (CVB-D) is the main active component of the traditional Chinese medicine Buxus microphylla. In the present study, we test the effects of CVB-D on gastric cancer cells and the underlying mechanisms of action. CVB-D reduced cell viability and colony formation ability of MGC-803 and MKN28 cells in a time- and concentration-dependent manner. Flow cytometry showed that cell cycle of CVB-D treated cells was arrested at the S-phase. CVB-D also induced apoptosis in MGC-803 and MKN28 cells, especially early stage apoptosis. Furthermore, mitochondria membrane potential (Δψm) was reduced and apoptosis-related proteins, cleaved Caspase-3 and Bax/Bcl-2, were up-regulated in CVB-D-treated MGC-803 and MKN28 cells. Taken together, our studies found that CVB-D plays important roles in inhibition of gastric tumorigenesis via arresting cell cycle and inducing mitochondria-mediated apoptosis, suggesting the potential application of CVB-D in gastric cancer therapy. View Full-Text
Keywords: cyclovirobuxine D; cell cycle; gastric cancer; apoptosis; mitochondria cyclovirobuxine D; cell cycle; gastric cancer; apoptosis; mitochondria
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wu, J.; Tan, Z.; Chen, J.; Dong, C. Cyclovirobuxine D Inhibits Cell Proliferation and Induces Mitochondria-Mediated Apoptosis in Human Gastric Cancer Cells. Molecules 2015, 20, 20659-20668.

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