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Molecules 2015, 20(11), 20518-20537; doi:10.3390/molecules201119712

Analysis of the Constituents in “Zhu She Yong Xue Shuan Tong” by Ultra High Performance Liquid Chromatography with Quadrupole Time-of-Flight Mass Spectrometry Combined with Preparative High Performance Liquid Chromatography

1
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China
2
Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 5 September 2015 / Revised: 27 October 2015 / Accepted: 6 November 2015 / Published: 18 November 2015
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [1949 KB, uploaded 18 November 2015]   |  

Abstract

“Zhu She Yong Xue Shuan Tong” lyophilized powder (ZSYXST), consists of a series of saponins extracted from Panax notoginseng, which has been widely used in China for the treatment of strokes. In this study, an ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) combined with preparative high performance liquid chromatography (PHPLC) method was developed to rapidly identify both major and minor saponins in ZSYXST. Some high content components were removed through PHPLC in order to increase the sensitivity of the trace saponins. Then, specific characteristic fragment ions in both positive and negative mode were utilized to determine the types of aglycone, saccharide, as well as the saccharide chain linkages. As a result, 94 saponins, including 20 pairs of isomers and ten new compounds, which could represent higher than 98% components in ZSYXST, were identified or tentatively identified in commercial ZSYXST samples. View Full-Text
Keywords: ZSYXST; saponins; UHPLC-Q-TOF/MS ZSYXST; saponins; UHPLC-Q-TOF/MS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wang, L.-L.; Han, L.-F.; Yu, H.-S.; Sang, M.-M.; Liu, E.-W.; Zhang, Y.; Fang, S.-M.; Wang, T.; Gao, X.-M. Analysis of the Constituents in “Zhu She Yong Xue Shuan Tong” by Ultra High Performance Liquid Chromatography with Quadrupole Time-of-Flight Mass Spectrometry Combined with Preparative High Performance Liquid Chromatography. Molecules 2015, 20, 20518-20537.

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