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Molecules 2015, 20(11), 20254-20267; doi:10.3390/molecules201119688

Molecular Cloning, Expression Pattern and Genotypic Effects on Glucoraphanin Biosynthetic Related Genes in Chinese Kale (Brassica oleracea var. alboglabra Bailey)

Department of Hortscience, South China Agricultural University, Guangzhou 510642, China
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Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 15 October 2015 / Revised: 29 October 2015 / Accepted: 3 November 2015 / Published: 11 November 2015
(This article belongs to the Section Molecular Diversity)
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Abstract

Glucoraphanin is a plant secondary metabolite that is involved in plant defense and imparts health-promoting properties to cruciferous vegetables. In this study, three genes involved in glucoraphanin metabolism, branched-chain aminotransferase 4 (BCAT4), methylthioalkylmalate synthase 1 (MAM1) and dihomomethionine N-hydroxylase (CYP79F1), were cloned from Chinese kale (Brassica oleracea var. alboglabra Bailey). Sequence homology and phylogenetic analysis identified these genes and confirmed the evolutionary status of Chinese kale. The transcript levels of BCAT4, MAM1 and CYP79F1 were higher in cotyledon, leaf and stem compared with flower and silique. BCAT4, MAM1 and CYP79F1 were expressed throughout leaf development with lower transcript levels during the younger stages. Glucoraphanin content varied extensively among different varieties, which ranged from 0.25 to 2.73 µmol·g−1 DW (dry weight). Expression levels of BCAT4 and MAM1 were high at vegetative–reproductive transition phase, while CYP79F1 was expressed high at reproductive phase. BCAT4, MAM1 and CYP79F1 were expressed significantly high in genotypes with high glucoraphanin content. All the results provided a better understanding of the roles of BCAT4, MAM1 and CYP79F1 in the glucoraphanin biosynthesis of Chinese kale. View Full-Text
Keywords: Chinese kale; glucoraphanin; BCAT4; MAM1; CYP79F1 Chinese kale; glucoraphanin; BCAT4; MAM1; CYP79F1
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yin, L.; Chen, C.; Chen, G.; Cao, B.; Lei, J. Molecular Cloning, Expression Pattern and Genotypic Effects on Glucoraphanin Biosynthetic Related Genes in Chinese Kale (Brassica oleracea var. alboglabra Bailey). Molecules 2015, 20, 20254-20267.

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