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Molecules 2015, 20(10), 18083-18094; doi:10.3390/molecules201018083

Quantification of Coumarins in Aqueous Extract of Pterocaulon balansae (Asteraceae) and Characterization of a New Compound

Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, Porto Alegre, RS 90610-000, Brazil
Author to whom correspondence should be addressed.
Academic Editor: Pascal Richomme
Received: 13 August 2015 / Revised: 16 September 2015 / Accepted: 17 September 2015 / Published: 2 October 2015
(This article belongs to the Special Issue Coumarins, Xanthones and Related Compounds)
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Plants from the genus Pterocaulon are popularly used as antifungal and wound-healing agents. Such activities have been related to coumarins, which are abundant in those plants. Coumarins are soluble in organic solvents, such as hexane and dichloromethane, and some of them are also soluble in hot water. Considering that infusion and decoctions of these plants are used in traditional medicine, the aim of this study was to identify and quantify the coumarins in the aqueous extract of Pterocaulon balansae. The aqueous extract was obtained by dynamic maceration and the compounds were characterized by UPLC-UV-MS analysis. A new coumarin and 5-methoxy-6,7-methylenedioxycoumarin, used for validation of the analytical HPLC method were obtained by partition of the aqueous extract with n-hexane. The HPLC method validated was linear, specific, and precise. Seven coumarins were characterized in the aqueous extract in a range of 0.584–54 mg/g of dry plant material. The main compound, 5,6-dimethoxy-7-(3′-methyl-2′,3′-dihydroxybutyloxy)coumarin, is described for the first time in P. balansae together with a new compound, 5,6-dimethoxy-7-(2′,3′-epoxy-3′-methylbutyloxy)coumarin. View Full-Text
Keywords: coumarin; Pterocaulon balansae; HPLC; UPLC-UV-MS; 5-methoxy-6,7-methylenedioxycoumarin; 5,6-dimethoxy-7-(2′,3′-epoxy-3′-methylbutyloxy)coumarin coumarin; Pterocaulon balansae; HPLC; UPLC-UV-MS; 5-methoxy-6,7-methylenedioxycoumarin; 5,6-dimethoxy-7-(2′,3′-epoxy-3′-methylbutyloxy)coumarin

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Medeiros-Neves, B.; de Barros, F.M.C.; von Poser, G.L.; Teixeira, H.F. Quantification of Coumarins in Aqueous Extract of Pterocaulon balansae (Asteraceae) and Characterization of a New Compound. Molecules 2015, 20, 18083-18094.

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