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Molecules 2015, 20(1), 951-973; doi:10.3390/molecules20010951

The Lectin Frontier Database (LfDB), and Data Generation Based on Frontal Affinity Chromatography

1
Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Central-2, 1-1-1, Umezono, Tsukuba, Ibaraki 305-8568, Japan
2
Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central-2, 1-1-1, Umezono, Tsukuba, Ibaraki 305-8568, Japan
3
Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236 Tangi-machi, Hachioji, Tokyo 192-8577, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Tzi Bun NG
Received: 24 November 2014 / Accepted: 31 December 2014 / Published: 8 January 2015
(This article belongs to the Special Issue Lectins)
View Full-Text   |   Download PDF [1667 KB, uploaded 8 January 2015]   |  

Abstract

Lectins are a large group of carbohydrate-binding proteins, having been shown to comprise at least 48 protein scaffolds or protein family entries. They occur ubiquitously in living organisms—from humans to microorganisms, including viruses—and while their functions are yet to be fully elucidated, their main underlying actions are thought to mediate cell-cell and cell-glycoconjugate interactions, which play important roles in an extensive range of biological processes. The basic feature of each lectin’s function resides in its specific sugar-binding properties. In this regard, it is beneficial for researchers to have access to fundamental information about the detailed oligosaccharide specificities of diverse lectins. In this review, the authors describe a publicly available lectin database named “Lectin frontier DataBase (LfDB)”, which undertakes the continuous publication and updating of comprehensive data for lectin-standard oligosaccharide interactions in terms of dissociation constants (Kd’s). For Kd determination, an advanced system of frontal affinity chromatography (FAC) is used, with which quantitative datasets of interactions between immobilized lectins and >100 fluorescently labeled standard glycans have been generated. The FAC system is unique in its clear principle, simple procedure and high sensitivity, with an increasing number (>67) of associated publications that attest to its reliability. Thus, LfDB, is expected to play an essential role in lectin research, not only in basic but also in applied fields of glycoscience. View Full-Text
Keywords: LfDB; frontal affinity chromatography (FAC); lectin-oligosaccharide interaction; dissociation constant (Kd); Pfam; scaffold; dynamic equilibrium LfDB; frontal affinity chromatography (FAC); lectin-oligosaccharide interaction; dissociation constant (Kd); Pfam; scaffold; dynamic equilibrium
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hirabayashi, J.; Tateno, H.; Shikanai, T.; Aoki-Kinoshita, K.F.; Narimatsu, H. The Lectin Frontier Database (LfDB), and Data Generation Based on Frontal Affinity Chromatography. Molecules 2015, 20, 951-973.

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