Next Article in Journal
Constituents of the Roots and Leaves of Ekebergia capensis and Their Potential Antiplasmodial and Cytotoxic Activities
Previous Article in Journal
1,2-Substituted 4-(1H)-Quinolones: Synthesis, Antimalarial and Antitrypanosomal Activities in Vitro
Article Menu

Export Article

Open AccessArticle
Molecules 2014, 19(9), 14221-14234; doi:10.3390/molecules190914221

Enhanced Production of Botrallin and TMC-264 with in Situ Macroporous Resin Adsorption in Mycelial Liquid Culture of the Endophytic Fungus Hyalodendriella sp. Ponipodef12

1
MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
2
Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
*
Author to whom correspondence should be addressed.
Received: 22 July 2014 / Accepted: 3 September 2014 / Published: 10 September 2014
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [702 KB, uploaded 10 September 2014]   |  

Abstract

Hyalodendriella sp. Ponipodef12, an endophytic fungus from the hybrid “Neva” of Populus deltoides × P. nigra, is a high producer of the bioactive dibenzo-α-pyrones botrallin and TMC-264. However, both the botrallin and TMC-264 produced by Hyalodendriella sp. Ponipodef12 were retained as both intracellular and extracellular products. The aim of this study was to evaluate an in situ macroporous resin adsorption for enhancement of botrallin and TMC-264 production in mycelial liquid culture of Hyalodendriella sp. Ponipodef12. Production of botrallin and TMC-264 was most effectively enhanced by macroporous resin DM-301 among the thirteen nonionic macroporous resins tested. The highest botrallin yield (51.47 mg/L, which was 2.29-fold higher than the control at 22.49 mg/L) was obtained by adding resin DM-301 at 4.38% (g/mL) to the culture broth on day 24 and allowing a period of 4 days for adsorption. The highest TMC-264 yield reached 47.74 mg/L, which was 11.76-fold higher than that of the control (4.06 mg/L), and was achieved by adding DM-301 resin at 4.38% (w/v) in the culture broth on day 24 and allowing a period of 6 days for adsorption. The results show that in situ resin adsorption is an effective strategy for enhancing production of botrallin and TMC-264, and also for facilitating their recovery from mycelial liquid culture of Hyalodendriella sp. Ponipodef12. View Full-Text
Keywords: dibenzo-α-pyrone; botrallin; TMC-264; endophytic fungus; Hyalodendriella sp. Ponipodef12; fermentation; macroporous resin; in situ resin adsorption dibenzo-α-pyrone; botrallin; TMC-264; endophytic fungus; Hyalodendriella sp. Ponipodef12; fermentation; macroporous resin; in situ resin adsorption
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Luo, H.; Liu, H.; Cao, Y.; Xu, D.; Mao, Z.; Mou, Y.; Meng, J.; Lai, D.; Liu, Y.; Zhou, L. Enhanced Production of Botrallin and TMC-264 with in Situ Macroporous Resin Adsorption in Mycelial Liquid Culture of the Endophytic Fungus Hyalodendriella sp. Ponipodef12. Molecules 2014, 19, 14221-14234.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top