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Molecules 2014, 19(8), 12640-12659; doi:10.3390/molecules190812640

Ultrasound-Assisted Extraction of Antioxidants in Misai Kucing (Orthosiphon stamineus)

1
Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM 43400, Serdang, Selangor, Malaysia
2
School of Science, Monash University Malaysia, Bandar Sunway, Petaling Jaya 46150, Selangor, Malaysia
3
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM 43400, Serdang, Selangor, Malaysia
4
Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, Cheras 56000, Kuala Lumpur, Malaysia
*
Author to whom correspondence should be addressed.
Received: 9 July 2014 / Revised: 11 August 2014 / Accepted: 11 August 2014 / Published: 19 August 2014
(This article belongs to the Section Natural Products)
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Abstract

Ultrasound-assisted extraction (UAE) with ethanol was used to extract the compounds responsible for the antioxidant activities of Misai Kucing (Orthosiphon stamineus). Response surface methodology (RSM) was used to optimize four independent variables: ethanol concentration (%), amplitude (%), duty cycle (W/s) and extraction time (min). Antioxidant compounds were determined by total phenolic content and total flavonoid content to be 1.4 g gallic acid equivalent/100 g DW and 45 g catechin equivalent/100 g DW, respectively. Antioxidant activities were evaluated using the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) radical scavenging capacity assay and the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging capacity assay to be 1,961.3 and 2,423.3 µmol Trolox Equivalent Antioxidant Capacity (TEAC)/100 g DW, respectively. Based on the optimal conditions, experimental values were reported to be close to the predicted value by RSM modeling (p > 0.05), indicating the suitability of UAE for extracting the antioxidants of Misai Kucing. Rosmarinic acid, kaempferol-rutinoside and sinesetine were identified by high performance liquid chromatography-mass spectrometry. View Full-Text
Keywords: ultrasound; extraction; antioxidants; Misai Kucing; Orthosiphon stamineus ultrasound; extraction; antioxidants; Misai Kucing; Orthosiphon stamineus
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MDPI and ACS Style

Ho, S.K.; Tan, C.P.; Thoo, Y.Y.; Abas, F.; Ho, C.W. Ultrasound-Assisted Extraction of Antioxidants in Misai Kucing (Orthosiphon stamineus). Molecules 2014, 19, 12640-12659.

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