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Molecules 2014, 19(8), 11560-11571; https://doi.org/10.3390/molecules190811560

Active Components with Inhibitory Activities on IFN-γ/STAT1 and IL-6/STAT3 Signaling Pathways from Caulis Trachelospermi

1
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
2
Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China
*
Author to whom correspondence should be addressed.
Received: 26 June 2014 / Revised: 25 July 2014 / Accepted: 27 July 2014 / Published: 5 August 2014
(This article belongs to the Section Natural Products Chemistry)
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Abstract

Initial investigation for new active herbal extract with inhibiting activity on JAK/STAT signaling pathway revealed that the extract of Caulis Trachelospermi, which was separated by 80% alcohol extraction and subsequent HP-20 macroporous resin column chromatography, was founded to strongly inhibit IFN-γ-induced STAT1-responsive luciferase activity (IFN-γ/STAT1) with IC50 value of 2.43 μg/mL as well as inhibiting IL-6-induced STAT3-responsive luciferase activity (IL-6/STAT3) with IC50 value of 1.38 μg/mL. Subsequent study on its active components led to the isolation and identification of two new dibenzylbutyrolactone lignans named 4-demethyltraxillaside (1) and nortrachelogenin 4-O-β-d-glucopyranoside (2), together with six known compounds. The lignan compounds 14 together with other lignan compounds isolated in previous study were tested the activities on IFN-γ/STAT1 and IL-6/STAT3 pathways. The following result showed that the main components trachelogenin and arctigenin had corresponding activities on IFN-γ/STAT1 pathway with IC50 values of 3.14 μM and 9.46 μM as well as trachelogenin, arctigenin and matairesinol strongly inhibiting IL-6/STAT3 pathway with IC50 values of 3.63 μM, 6.47 μM and 2.92 μM, respectively. View Full-Text
Keywords: Caulis Trachelospermi; 4-demethyltraxillaside; nortrachelogenin 4-O-β-d-glucopyranoside; JAK/STAT signaling pathway Caulis Trachelospermi; 4-demethyltraxillaside; nortrachelogenin 4-O-β-d-glucopyranoside; JAK/STAT signaling pathway
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Liu, X.-T.; Wang, Z.-X.; Yang, Y.; Wang, L.; Sun, R.-F.; Zhao, Y.-M.; Yu, N.-J. Active Components with Inhibitory Activities on IFN-γ/STAT1 and IL-6/STAT3 Signaling Pathways from Caulis Trachelospermi. Molecules 2014, 19, 11560-11571.

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