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Molecules 2014, 19(8), 10717-10732; doi:10.3390/molecules190810717

Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola

1
Korea Research Institute of Bioscience and Biotechnology, Ochang, Chungbuk 363-883, Korea
2
Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek 94598, CA, USA
3
Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Buk-Gu, Gwangju 500-757, Korea
*
Authors to whom correspondence should be addressed.
Received: 8 May 2014 / Revised: 9 July 2014 / Accepted: 10 July 2014 / Published: 24 July 2014
(This article belongs to the Special Issue Phytoalexins: Current Progress and Future Prospects)
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Abstract

Brassica species produce the antifungal indolyl compounds brassinin and its derivatives, during microbial infection. The fungal pathogen Alternaria brassicicola detoxifies brassinin and possibly its derivatives. This ability is an important property for the successful infection of brassicaceous plants. Previously, we identified a transcription factor, Bdtf1, essential for the detoxification of brassinin and full virulence. To discover genes that encode putative brassinin-digesting enzymes, we compared gene expression profiles between a mutant strain of the transcription factor and wild-type A. brassicicola under two different experimental conditions. A total of 170 and 388 genes were expressed at higher levels in the mutants than the wild type during the infection of host plants and saprophytic growth in the presence of brassinin, respectively. In contrast, 93 and 560 genes were expressed, respectively, at lower levels in the mutant than the wild type under the two conditions. Fifteen of these genes were expressed at lower levels in the mutant than in the wild type under both conditions. These genes were assumed to be important for the detoxification of brassinin and included Bdtf1 and 10 putative enzymes. This list of genes provides a resource for the discovery of enzyme-coding genes important in the chemical modification of brassinin. View Full-Text
Keywords: phytoalexin; brassinin; gene expression profiles; RNA-seq; necrotrophic fungus; phytoalexin detoxification; GEO Series Accession No. GSE59195 phytoalexin; brassinin; gene expression profiles; RNA-seq; necrotrophic fungus; phytoalexin detoxification; GEO Series Accession No. GSE59195
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MDPI and ACS Style

Cho, Y.; Ohm, R.A.; Devappa, R.; Lee, H.B.; Grigoriev, I.V.; Kim, B.Y.; Ahn, J.S. Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola. Molecules 2014, 19, 10717-10732.

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