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Formation of a Flavin-Linked Peptide
AbstractIn a previous study, we showed that formylmethylflavin (FMF) can bind to cysteine. In this study, FMF was reacted with native peptides (CG and CKLVFF) containing an N-terminal cysteine. The formation of flavin-CG and flavin-CKLVFF was confirmed using HPLC and ESI-MS. Storage of flavin-CKLVFF in DMSO at −30 °C for 7 days resulted in no detectable deposition. In contrast, flavin-CKLVFF formed deposits when stored in water at −30 °C for 1 day, but no deposit was observed in the aqueous solution of flavin-CKLVFF after 7 days storage in the presence of 0.1% Triton X-100.
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Morikawa, M.; Kino, K.; Senda, T.; Suzuki, M.; Kobayashi, T.; Miyazawa, H. Formation of a Flavin-Linked Peptide. Molecules 2014, 19, 9552-9561.View more citation formats
Morikawa M, Kino K, Senda T, Suzuki M, Kobayashi T, Miyazawa H. Formation of a Flavin-Linked Peptide. Molecules. 2014; 19(7):9552-9561.Chicago/Turabian Style
Morikawa, Masayuki; Kino, Katsuhito; Senda, Takeshi; Suzuki, Masayo; Kobayashi, Takanobu; Miyazawa, Hiroshi. 2014. "Formation of a Flavin-Linked Peptide." Molecules 19, no. 7: 9552-9561.