Next Article in Journal
Ultrasonic Extraction of Antioxidants from Chinese Sumac (Rhus typhina L.) Fruit Using Response Surface Methodology and Their Characterization
Next Article in Special Issue
Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB
Previous Article in Journal
MAPLE Fabricated Fe3O4@Cinnamomum verum Antimicrobial Surfaces for Improved Gastrostomy Tubes
Previous Article in Special Issue
Polyhydroyxalkanoate Synthase Fusions as a Strategy for Oriented Enzyme Immobilisation
Molecules 2014, 19(7), 8995-9018; doi:10.3390/molecules19078995

Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases

* ,
University of Silesia in Katowice, Faculty of Biology and Environmental Protection, Department of Biochemistry, Jagiellonska 28, 40-032 Katowice, Poland
* Author to whom correspondence should be addressed.
Received: 12 May 2014 / Revised: 13 June 2014 / Accepted: 16 June 2014 / Published: 27 June 2014
(This article belongs to the Special Issue Enzyme Immobilization)
View Full-Text   |   Download PDF [858 KB, uploaded 27 June 2014]   |   Browse Figures


The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properties of enzymes such as performance in organic solvents, pH tolerance, heat stability or the functional stability. Increasing the structural rigidity of the protein and stabilization of multimeric enzymes which prevents dissociation-related inactivation. In the last decade, several papers about immobilization methods have been published. In our work, we present a relation between the influence of immobilization on the improvement of the properties of selected oxidoreductases and their commercial value. We also present our view on the role that different immobilization methods play in the reduction of enzyme inhibition during biotechnological processes.
Keywords: oxidoreductases; immobilization; stability of enzyme oxidoreductases; immobilization; stability of enzyme
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Guzik, U.; Hupert-Kocurek, K.; Wojcieszyńska, D. Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases. Molecules 2014, 19, 8995-9018.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert