Next Article in Journal
Ultrasonic Extraction of Antioxidants from Chinese Sumac (Rhus typhina L.) Fruit Using Response Surface Methodology and Their Characterization
Next Article in Special Issue
Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB
Previous Article in Journal
MAPLE Fabricated Fe3O4@Cinnamomum verum Antimicrobial Surfaces for Improved Gastrostomy Tubes
Previous Article in Special Issue
Polyhydroyxalkanoate Synthase Fusions as a Strategy for Oriented Enzyme Immobilisation
Article Menu

Export Article

Open AccessReview
Molecules 2014, 19(7), 8995-9018; doi:10.3390/molecules19078995

Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases

University of Silesia in Katowice, Faculty of Biology and Environmental Protection, Department of Biochemistry, Jagiellonska 28, 40-032 Katowice, Poland
*
Author to whom correspondence should be addressed.
Received: 12 May 2014 / Revised: 13 June 2014 / Accepted: 16 June 2014 / Published: 27 June 2014
(This article belongs to the Special Issue Enzyme Immobilization)
View Full-Text   |   Download PDF [858 KB, uploaded 27 June 2014]   |  

Abstract

The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properties of enzymes such as performance in organic solvents, pH tolerance, heat stability or the functional stability. Increasing the structural rigidity of the protein and stabilization of multimeric enzymes which prevents dissociation-related inactivation. In the last decade, several papers about immobilization methods have been published. In our work, we present a relation between the influence of immobilization on the improvement of the properties of selected oxidoreductases and their commercial value. We also present our view on the role that different immobilization methods play in the reduction of enzyme inhibition during biotechnological processes.
Keywords: oxidoreductases; immobilization; stability of enzyme oxidoreductases; immobilization; stability of enzyme
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Guzik, U.; Hupert-Kocurek, K.; Wojcieszyńska, D. Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases. Molecules 2014, 19, 8995-9018.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top