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Molecules 2014, 19(7), 10534-10545; https://doi.org/10.3390/molecules190710534

Reduction of Adhesion Molecule Production and Alteration of eNOS and Endothelin-1 mRNA Expression in Endothelium by Euphorbia hirta L. through Its Beneficial β-Amyrin Molecule

1
Department of Pharmacy, Chia-Nan University of Pharmacy & Science, Tainan 717, Taiwan
2
Department of Chemistry and Biochemistry, National Chung Cheng University, Chia-Yi 621, Taiwan
*
Author to whom correspondence should be addressed.
Received: 6 June 2014 / Revised: 3 July 2014 / Accepted: 11 July 2014 / Published: 18 July 2014
(This article belongs to the Section Natural Products Chemistry)
Full-Text   |   PDF [519 KB, uploaded 18 July 2014]   |  

Abstract

The inflammatory reaction in large blood vessels involves up-regulation of vascular adhesion molecules such as endothelial cell selectin (E-selectin), soluble vascular cell adhesion molecule (sVCAM)-1, and soluble intercellular adhesion molecule (sICAM)-1. These vascular dysfunctions are associated with the development of atherosclerosis. β-Amyrin, an active component of Euphorbia hirta L., has potent anti-inflammatory effects. So far, its preventive effects against the expression of inflammatory mediator-induced adhesion molecules have not been investigated. Endothelial cells (SVEC4-10 cell line) were treated with 50% RAW conditioned media (i.e., normal SVEC4-10 culture media contains 50% of lipopolysaccharide-activated macrophage culture media) without or with β-amyrin (0.6 and 0.3 µM). The production levels of E-selectin, sICAM-1, and sVCAM-1 in the SVEC4-10 cells were measured with ELISA assay kits. Under the same treatment conditions, expression of endothelin (ET)-1 and endothelial type of NO synthase (eNOS) mRNA were analyzed by RT-PCR and agarose gel. With β-amyrin, the 50% RAW conditioned media-induced E-selectin, sICAM-1, and sVCAM-1 levels as well as ET-1 gene expression were all suppressed. β-Amyrin treatment also restored the 50% RAW conditioned media-suppressed eNOS mRNA expression. These data indicate that β-amyrin is potentially useful in preventing chronic inflammation-related vascular diseases. View Full-Text
Keywords: E-selectin; sICAM-1; sVCAM-1; Endothelin-1 mRNA; eNOS mRNA; β-amyrin E-selectin; sICAM-1; sVCAM-1; Endothelin-1 mRNA; eNOS mRNA; β-amyrin
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Shih, M.F.; Cherng, J.Y. Reduction of Adhesion Molecule Production and Alteration of eNOS and Endothelin-1 mRNA Expression in Endothelium by Euphorbia hirta L. through Its Beneficial β-Amyrin Molecule. Molecules 2014, 19, 10534-10545.

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