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Article

Capsaicin: A Potent Inhibitor of Carbonic Anhydrase Isoenzymes

by
Betul Arabaci
1,
Ilhami Gulcin
1,2,* and
Saleh Alwasel
2
1
Atatürk University, Faculty of Sciences, Department of Chemistry, Erzurum 25240, Turkey
2
Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
*
Author to whom correspondence should be addressed.
Molecules 2014, 19(7), 10103-10114; https://doi.org/10.3390/molecules190710103
Submission received: 6 May 2014 / Revised: 19 June 2014 / Accepted: 3 July 2014 / Published: 10 July 2014
(This article belongs to the Section Natural Products Chemistry)
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Abstract

:
Carbonic anhydrase (CA, EC 4.2.1.1) is a zinc containing metalloenzyme that catalyzes the rapid and reversible conversion of carbon dioxide (CO2) and water (H2O) into a proton (H+) and bicarbonate (HCO3–) ion. On the other hand, capsaicin is the main component in hot chili peppers and is used extensively used in spices, food additives and drugs; it is responsible for their spicy flavor and pungent taste. There are sixteen known CA isoforms in humans. Human CA isoenzymes I, and II (hCA I and hCA II) are ubiquitous cytosolic isoforms. In this study, the inhibition properties of capsaicin against the slow cytosolic isoform hCA I, and the ubiquitous and dominant rapid cytosolic isozymes hCA II were studied. Both CA isozymes were inhibited by capsaicin in the micromolar range. This naturally bioactive compound has a Ki of 696.15 µM against hCA I, and of 208.37 µM against hCA II.

Graphical Abstract

1. Introduction

Carbonic anhydrase (CA) enzymes are virtually ubiquitous in all living systems and have important roles in pH regulation, carboxylation reactions, fluid balance, bone resorption, tumorigenicity, calcification, the synthesis of bicarbonate and in many other pathological and physiological processes [1,2,3,4]. CA catalyzes the reversible hydration of carbon dioxide (CO2) and water (H2O) to bicarbonate (HCO3−) and a proton (H+) [5,6,7,8,9].
CO2 + H2O ⇔ H2CO3 ⇔ HCO3− + H+
An enzyme inhibitor is a molecule that binds to an enzyme and decreases its activity. An inhibitor can prevent a substrate from entering the active site of the enzyme or hindering catalysis. It was well known that carbonic anhydrase inhibitors (CAIs) bind to a catalytic Zn2+ ion in the active site of CA isoenzymes and block their activity [10,11,12,13,14,15]. CAIs are clinically used to treat glaucoma, and as anticonvulsant agents [15], diuretics [6] and antiobesity drugs [16]. Additionally, they have recently been used in the management of hypoxic tumors [17]. The first aromatic and heterocyclic sulfonamides were clinically used derivatives of acetazolamide [18]. As seen in Scheme 1, to regenerate the basic form of the enzyme, a proton is transferred from the active site to the solvent. This proton transfer may be assisted by active site residues or by buffers present in the medium. The fourth position is occupied by H2O at acidic pH, and is catalytically inactive. At higher pH, the water molecule binds to Zn2+ within the CA active site, and the proton transfer reaction transfers a proton to the solvent, leaving an -OH [11,14].
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Scheme 1. Schematic presentation of the catalytic inhibition mechanism for the CA catalyzed CO2 hydration.
Scheme 1. Schematic presentation of the catalytic inhibition mechanism for the CA catalyzed CO2 hydration.
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Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) comes from the plants of genus Capsicum and is a pungent constituent of capsicum fruits, which are used extensively in condiments in Asian, African, and Latin American countries [19]. It is also used in a number of over-the-counter weight loss products because of its potential role in increasing metabolic activity [20]. It is the active and pungent ingredient in a wide variety of red peppers [21]. Because of its characteristic smell and taste, capsaicin is one of the most heavily consumed additives throughout the world [22].
Capsaicin is a naturally occurring alkaloid obtained from red peppers. It is generally isolated from Capsicum annuum and is responsible for the spiciness hotness of chili. Capsaicin and related compounds are referred to as capsaicinoids and are produced as secondary metabolites by red peppers [23]. The total intake of capsaicinoid compounds in Asian countries is estimated to be 25–200 mg/day per person given that as capsaicin accounts for 80% of the content of capsicum fruits [24]. In this study, we identify the potential inhibition profile and mechanism for human CA isoenzymes I, and II (hCA I, and II), which are widely used in the food and pharmaceutical industries.

2. Results and Discussion

Capsaicin is a monophenolic compound with an amide and lipophilic carbon chain on one end and a hydrophilic ring on the other. It is an amide derivative of vanillylamine and trans-8-methylnon-6-enoic acid. The vanillylamine moiety of capsaicin is biologically synthesized from phenylalanine. The fatty acid moiety at the other end is derived from valine [21]. Capsaicin is employed as an agricultural repellent and as an additive or colorant in the cosmetic, food and pharmaceutical industries. It has multiple pharmacological activities, including anti-inflammatory, anticancer [21], genotoxic and chemopreventive [25], antifungal and analgesic [26], neuroprotective [27], antiobesity [28], anti-apoptotic [29], and anti-epileptic effects and antioxidant activities [30]. Capsaicin is also useful in combating liver and duodenal cancers [31]. Previous research studies have shown that capsaicin induces apoptosis and cell-cycle arrest and inhibits cell proliferation in a variety of cancer cells [32]. Furthermore, it stimulates apoptotic cell death in rat trigeminal primary neurons when administered during the neonatal period [33].
It works to speed up the body’s metabolism by activation of the sympathetic nervous system [34]. It has been shown that, in comparison to a control capsaicin supplementation control during a negative energy balance counteracts the normal decrease in energy expenditure. Moreover, the consumption of capsaicin promotes fat oxidation in negative energy balance and does not significantly increase blood pressure [35]. The chemical structure of capsaicin is shown in Figure 1, as well as an estimated binding model of capsaicin to the active site of CA. A second hydrogen bond has been modeled between the oxygen atom of phenol moiety of capsaicin and the amide NH of Thr199, an amino acid residue that is universally conserved in CAs. Thus, by binding in a non-classical way to CAs, phenols and their derivatives provide interesting leads for identifying novel types of CAIs. Capsaicin has three classical structural characteristics in one molecule, an amide with a lipophilic carbon chain on one end and a hydrophilic ring on the other. There are a large number of capsaicin analogs that contain the same basic functional groups but possess variations in one or more of the three structural characteristics [36].
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Figure 1. The proposed binding model of capsaicin to CA by floating to the Zn2+ coordinated water/hydroxide ion (-OH).
Figure 1. The proposed binding model of capsaicin to CA by floating to the Zn2+ coordinated water/hydroxide ion (-OH).
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CAs are involved in crucial physiological processes connected with CO2/HCO3− transport and homeostasis, electrolyte secretion in a variety of tissues and organs, biosynthetic reactions including gluconeogenesis, ureagenesis and lipogenesis, respiration, calcification, tumorigenicity, and bone resorption [37]. Phenolic compounds are a class of chemicals containing of an -OH bonded directly to an aromatic hydrocarbon group and are categorized either as simple phenols or as polyphenols depending on the number of phenol units in the molecule [38,39]. Phenols, phenolic acid and phenolic derivatives were recently investigated in detail as inhibitors of the Zn2+-containing CA [40,41,42,43]. All CA isoforms are inhibited by three different mechanisms: (i) by coordination of the inhibitor to the Zn2+ from the active site of CA and replacing the Zn2+-bound H2O/-OH, which leads to a tetrahedral geometry for Zn2+. This geometry can also arise by the addition an inhibitor to the metal coordination sphere when the Zn2+ has in trigonal bipyramidal geometry [1]; (ii) by floating of the inhibitor to the Zn2+-bound solvent molecule, i.e., an H2O/-OH. Phenolic compounds and polyamine molecules can bind CA in this way, as shown schematically for phenol; or (iii) by inhibitor occlusion of the entrance to the active site or activator-binding site of CA [44,45,46,47].
It has been reported that simple phenol acts as an inhibitor of the Zn2+ containing CA isoenzymes [6,8,43,44]. Phenol binds to CA in a diverse manner when compared to the classic sulfonamide inhibitors. Sulfonamides coordinate to the Zn2+ ion in the CA active site by replacing the fourth non-protein ligand, which is typically a H2O molecule or -OH ion. By binding in a non-classical way to CAs, phenols and their derivatives constitute interesting leads for identifying novel types of CAIs [37,45,46,47]. In the present study, we report the inhibition profiles of capsaicin against the slower cytosolic isoform hCA I, and the more rapid isozymes hCA II. Capsaicin showed effective inhibition both isoforms. When examining the results, the following structure activity relationship could be easily observed.
To describe inhibitory effects, researchers often list an IC50 value; however, a more suitable measure is the Ki constant. Ki values were calculated from Lineweaver-Burk graphs (Figure 2), and both the Ki and IC50 parameters of the capsaicin were determined in this study. As shown in Table 1, Figure 2 and Figure 3, the Ki values for capsaicin were found, and the corresponding IC50 values were calculated for each CA isoenzyme. For the cytosolic isoenzyme hCA I capsaicin had an IC50 values of 428.04 µM and Ki values of 696.15 ± 59.37 µM (Table 1). For the physiologically predominant CA II, capsaicin had IC50 values of 316.01 µM and Ki values of 208.37 ± 14.38 µM. Many studies have shown that the inhibition of CA II is brought about by an inhibitor’s ability to bind to catalytic Zn2+ in the CA’s active site and mimic to tetrahedral transition state [6,11,14]. Thus, in Figure 1, we illustrate a binding model between capsaicin and the enzyme’s active site. There are important differences in inhibition between the two isoenzymes. The main difference in the active site architectures of two isozymes is due to the presence of more histidine residues in the CA I isoform [14]. In addition to the Zn2+ ligands (His 94, His 96, and His 119), discussed in the introduction, His 64 of CA I plays an important role in catalysis.
Molecules 19 10103 g002 550
Figure 2. Determination of Ki values of capsaicin for human erythrocyte carbonic anhydrase I (A), and II (B) isoenzymes (hCA I, and II) by Lineweaver-Burk plots.
Figure 2. Determination of Ki values of capsaicin for human erythrocyte carbonic anhydrase I (A), and II (B) isoenzymes (hCA I, and II) by Lineweaver-Burk plots.
Molecules 19 10103 g002
Molecules 19 10103 g003 550
Figure 3. The effects of different concentrations of capsaicin on human erythrocyte carbonic anhydrase I (A), and II (B) isoenzymes (hCA I, and II).
Figure 3. The effects of different concentrations of capsaicin on human erythrocyte carbonic anhydrase I (A), and II (B) isoenzymes (hCA I, and II).
Molecules 19 10103 g003
Table
Table 1. The inhibition profile of capsaicin on purified hCA I, and hCA II from human erythrocytes by Sepharose-4B-l-tyrosine-sulfanilamide affinity chromatography.
Table 1. The inhibition profile of capsaicin on purified hCA I, and hCA II from human erythrocytes by Sepharose-4B-l-tyrosine-sulfanilamide affinity chromatography.
Kinetic ParametershCA IhCA II
IC50 (µM)428.04316.01
Ki (µM)696.15 ± 59.37208.37 ± 14.38
Inhibition typeUncompetitiveUncompetitive
Another important difference between the two isozymes is that CA II contains a histidine cluster, consisting of the following residues: His 64, His 4 His 3, His 10, His 15, and His 17 which is absent in CA I. Hence, these two isozymes exhibit different affinities for the inhibitors. In general, CA II has a higher affinity for the inhibitor than CA I [14].
hCA I is highly abundant in red blood cells and found in many tissues although its precise physiological function is unknown. CA I is associated with cerebral and retinal edema, and the inhibition of CA I inhibition may be a valuable tool for fighting these conditions. The physiologically predominant cytosolic isoform hCA II is ubiquitous and associated with several diseases including epilepsy, edema, glaucoma, and altitude sickness [17,48].
Acetazolamide is a well-known example of a clinically established carbonic anhydrase inhibitor [49,50] and in recent years we have reported on the strong inhibition of both human cytosolic CA I, and II. CA inhibitory effects are also exhibited by a wide spectrum of phenolic compounds including melatonin [9], morphine [51], vitamin E [52], CAPE [50], antioxidant phenols [47], phenolic acids [41], natural product polyphenols and phenolic acids [43], natural phenolic compounds [10], antioxidant polyphenol products [45,46], (3,4-dihydroxyphenyl)(2,3,4-trihydroxyphenyl)methanone and its derivatives [13], natural and synthetic bromophenols [53], novel sulfonamide derivatives of aminoindanes and aminotetralins [7], novel phenolic sulfamides [54], novel phenolic benzylamine derivatives [5], novel sulfamide analogues of dopamine related compounds [3], new benzotropone derivatives [11], brominated diphenylmethanone and its derivatives [55] and novel sulfamides and sulfonamides incorporating tetralin scaffold [4] have been reported. These extensive studies indicate the importance of CA I, and II isoenzyme inhibitors.

3. Experimental

Biochemistry
Both of the CA isoenzymes were purified by Sepharose-4B-L tyrosine-sulphanilamide affinity chromatography [51] accordance to previous studies [52,56]. For purification, the lysate was adjusted to pH 8.7 with Tris. Then, an aliquot of the lysate was applied to the affinity column and proteins content in the eluates was observed spectrophotometrically at 280 nm. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed after purification of the enzymes. The isoenzymes purities were determined by SDS-PAGE and a single band was observed for each CA isoenzyme [57]. This method has been described previously [58] and was performed using acrylamide in the running (10%) and the stacking gel (3%) with SDS (0.1%) [59,60].
Both CA isoenzyme activities were determined according to Verpoorte et al. [61] as described previously [62,63]. The absorbance change at 348 nm was observed over a period of 3 min at room temperature (25 °C) using a spectrophotometer (UVmini-1240 UV-VIS spectrophotometer, Shimadzu, Kyoto, Japan), before and after adding the sample. One unit of enzyme activity was expressed as 1 mol/L of released p-nitrophenol per minute at 25 °C [64]. The quantity of protein was spectrophotometrically determined at 595 nm during purification the Bradford method [65] and bovine serum albumin (BSA) was used as the standard protein [66,67,68].
The inhibition effect of capsaicin on CA isoenzymes was measured the hydrolysis of p-nitrophenyl acetate (PNA) by CA to p-nitrophenol; p-nitrophenol can be quantified spectrophotometrically [12]. The CA-catalysed reaction of CO2 hydration was first observed in the absence of capsaicin; the rates were measured and used as a control for the CA isoenzymes. Then the same reaction was measured in the presence of capsaicin. The percent inhibition was determined with (%) = [100 − (ATC/AC) × 100]; ATC is the absorbance of the sample containing capsaicin and AC is the absorbance of the control sample. Activity (%)-[capsaicin] graphs were drawn and the half maximal inhibitory concentration (IC50) values of capsaicin exhibiting more than 50% inhibition of CA were calculated after suitable dilutions. IC50 is a measure of the potency of capsaicin in inhibiting CA isoenzyme activity. In addition to these values, the Ki values for capsaicin were determined for each isoenzyme. To determine the Ki values, capsaicin was tested at three different concentrations. Ki is the binding affinity constant of the inhibitor. In these experiments, NPA was used as the substrate at five different concentrations and Lineweaver-Burk curves were drawn [69] in detail as described previously [70,71,72,73].

4. Conclusions

Capsaicin exhibited unique inhibition profiles against both CA isoform I, and II. These results indicate that, despite the high homology between these two CAs, they do not display similar activity. The logic of working with capsaicin was first to identify a potent CA inhibitor because phenolic compounds with aromatic rings have been previously identified as inhibitors of CA. In this study, micromolar levels of Ki and IC50 values in the micromolar range were observed for capsaicin. We show that capsaicin is a selective inhibitor of both cytosolic CA isoenzymes. These results clearly indicate the potential use for bioactive phenolic capsaicin in identifying more CA inhibitors and for eventually targeting additional isoforms.

Acknowledgments

The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding of this research through the Research Group Project No. RGP-VPP-254

Author Contributions

BG was responsible for the purification of enzyme and determination of inhibition effect of Capsaicin on CA isoenzymes. IG participated in the study design and coordination, drafted the manuscript. SA participated in the design of the study, helped to draft and reviewed the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

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  • Sample Availability: Samples of the compounds are available from the authors.

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MDPI and ACS Style

Arabaci, B.; Gulcin, I.; Alwasel, S. Capsaicin: A Potent Inhibitor of Carbonic Anhydrase Isoenzymes. Molecules 2014, 19, 10103-10114. https://doi.org/10.3390/molecules190710103

AMA Style

Arabaci B, Gulcin I, Alwasel S. Capsaicin: A Potent Inhibitor of Carbonic Anhydrase Isoenzymes. Molecules. 2014; 19(7):10103-10114. https://doi.org/10.3390/molecules190710103

Chicago/Turabian Style

Arabaci, Betul, Ilhami Gulcin, and Saleh Alwasel. 2014. "Capsaicin: A Potent Inhibitor of Carbonic Anhydrase Isoenzymes" Molecules 19, no. 7: 10103-10114. https://doi.org/10.3390/molecules190710103

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