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Molecules 2014, 19(6), 8644-8660; doi:10.3390/molecules19068644

Potential Anti-Inflammatory Effects of the Hydrophilic Fraction of Pomegranate (Punica granatum L.) Seed Oil on Breast Cancer Cell Lines

1
Istituto Nazionale per lo studio e la cura dei tumori "Fondazione Giovanni Pascale"-IRCCS–80131 Napoli, Italy
2
Istituto di Scienze dell' Alimentazione-CNR, Via Roma 64, 83100 Avellino, Italy
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 23 April 2014 / Revised: 9 June 2014 / Accepted: 18 June 2014 / Published: 24 June 2014
(This article belongs to the Section Medicinal Chemistry)
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Abstract

In this work, we characterized conjugated linolenic acids (e.g., punicic acid) as the major components of the hydrophilic fraction (80% aqueous methanol extract) from pomegranate (Punica granatum L.) seed oil (PSO) and evaluated their anti-inflammatory potential on some human colon (HT29 and HCT116), liver (HepG2 and Huh7), breast (MCF-7 and MDA-MB-231) and prostate (DU145) cancer lines. Our results demonstrated that punicic acid and its congeners induce a significant decrease of cell viability for two breast cell lines with a related increase of the cell cycle G0/G1 phase respect to untreated cells. Moreover, the evaluation of a great panel of cytokines expressed by MCF-7 and MDA-MB-231 cells showed that the levels of VEGF and nine pro-inflammatory cytokines (IL-2, IL-6, IL-12, IL-17, IP-10, MIP-1α, MIP-1β, MCP-1 and TNF-α) decreased in a dose dependent way with increasing amounts of the hydrophilic extracts of PSO, supporting the evidence of an anti-inflammatory effect. Taken together, the data herein suggest a potential synergistic cytotoxic, anti-inflammatory and anti-oxidant role of the polar compounds from PSO. View Full-Text
Keywords: anti-inflammatory effects; bioactive molecules; cell viability; cytokines; pomegranate (Punica granatum L.) anti-inflammatory effects; bioactive molecules; cell viability; cytokines; pomegranate (Punica granatum L.)
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Costantini, S.; Rusolo, F.; De Vito, V.; Moccia, S.; Picariello, G.; Capone, F.; Guerriero, E.; Castello, G.; Volpe, M.G. Potential Anti-Inflammatory Effects of the Hydrophilic Fraction of Pomegranate (Punica granatum L.) Seed Oil on Breast Cancer Cell Lines. Molecules 2014, 19, 8644-8660.

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