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Molecules 2014, 19(6), 8533-8543; doi:10.3390/molecules19068533

Cis-[Cr(C2O4)(pm)(OH2)2]+ Coordination Ion as a Specific Sensing Ion for H2O2 Detection in HT22 Cells

1
Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, Gdańsk 80-308, Poland
2
Department of Histology, Medical University of Gdańsk, Dębinki 1, Gdańsk 80-211, Poland
3
Department of Medical Chemistry, Medical University of Gdańsk, Dębinki 1, Gdańsk 80-211, Poland
*
Author to whom correspondence should be addressed.
Received: 8 April 2014 / Revised: 30 May 2014 / Accepted: 10 June 2014 / Published: 23 June 2014
(This article belongs to the Section Medicinal Chemistry)
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Abstract

The purpose of this study was to examine the application of the coordinated cis-[Cr(C2O4)(pm)(OH)2]+ cation where pm denotes pyridoxamine, as a specific sensing ion for the detection of hydrogen peroxide (H2O2). The proposed method for H2O2 detection includes two key steps. The first step is based on the nonenzymatic decarboxylation of pyruvate upon reaction with H2O2, while the second step is based on the interaction of cis-[Cr(C2O4)(pm)(OH2)2]+ with the CO2 released in the previous step. Using this method H2O2 generated during glutamate-induced oxidative stress was detected in HT22 hippocampal cells. The coordination ion cis-[Cr(C2O4)(pm)(OH2)2]+ and the spectrophotometric stopped-flow technique were applied to determine the CO2 concentration in cell lysates, supernatants and cell-free culture medium. Prior to CO2 assessment pyruvate was added to all samples studied. Pyruvate reacts with H2O2 with 1:1 stoichiometry, and consequently the amount of CO2 released in this reaction is equivalent to the amount of H2O2. View Full-Text
Keywords: glutamate; HT-22 cells; hydrogen peroxide; kinetic mechanism; Cr(III) complexes; pyridoxamine glutamate; HT-22 cells; hydrogen peroxide; kinetic mechanism; Cr(III) complexes; pyridoxamine
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Jacewicz, D.; Siedlecka-Kroplewska, K.; Pranczk, J.; Wyrzykowski, D.; Woźniak, M.; Chmurzyński, L. Cis-[Cr(C2O4)(pm)(OH2)2]+ Coordination Ion as a Specific Sensing Ion for H2O2 Detection in HT22 Cells. Molecules 2014, 19, 8533-8543.

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