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Molecules 2014, 19(6), 8112-8123; doi:10.3390/molecules19068112

Article Expression, Purification, and Characterization of Cu/ZnSOD from Panax Ginseng

1
Department of Gastrointestinal Surgery, China-Japan Union Hospital, Jilin University, Changchun 130000, China
2
Center of Chinese Medicine and Bio-Engineering Research, Changchun University of Chinese Medicine, Changchun 130117, China
3
Department of Rheumatoid Immune, First Hospital of Jilin University, Changchun 130000, China
4
Department of Geriatrics, China-Japan Union Hospital, Jilin University, Changchun 130000, China
*
Authors to whom correspondence should be addressed.
Received: 27 January 2014 / Revised: 25 May 2014 / Accepted: 5 June 2014 / Published: 16 June 2014
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Abstract

Superoxide dismutase (SOD) has a strong antioxidant effect, but the traditional SOD extraction method is not the most efficient method of SOD amplification. In this study, we report the cloning of the Cu/ZnSOD gene from Panax ginseng into a temperature-regulated expression plasmid, pBV220. Cu/ZnSOD inclusion bodies were expressed in E. coli at a high level. Then, the inclusion bodies were purified by ion-exchange chromatography and molecular sieve chromatography. Finally, we obtained stable SOD in the bacterial broth, with a protein content of 965 mg/L and enzyme specific activity of 9389.96 U/mg. These results provide a foundation for future studies on the antioxidant mechanisms of ginseng and the development and application of ginseng Cu/ZnSOD. View Full-Text
Keywords: ginseng; Cu/ZnSOD; pBV220; expression; purification ginseng; Cu/ZnSOD; pBV220; expression; purification
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Ding, D.; Liu, S.; Wang, K.; Huang, L.; Zhao, J. Article Expression, Purification, and Characterization of Cu/ZnSOD from Panax Ginseng. Molecules 2014, 19, 8112-8123.

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