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Molecules 2014, 19(6), 7568-7580; doi:10.3390/molecules19067568

Qualitative and Quantitative Analysis of Triterpene Saponins from Tea Seed Pomace (Camellia oleifera Abel) and Their Activities against Bacteria and Fungi

1
College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China
2
Key Laboratory of Tea Biochemistry & Biotechnology, Ministry of Education and Ministry of Agriculture, Anhui Agricultural University, Hefei 230036, China
3
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
*
Authors to whom correspondence should be addressed.
Received: 31 March 2014 / Revised: 27 May 2014 / Accepted: 3 June 2014 / Published: 6 June 2014
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Abstract

A method using LC-ESI-IT-TOF/MS and LC/UV-ELSD was established to qualitatively analyze triterpene saponins obtained from the tea seed pomace (Camellia oleifera Abel). In addition, the quantitative analysis of oleiferasaponin A1 using LC/UV was developed. The purified total saponins did not exhibit any inhibitory effects at concentrations ranging from 0.1 to 10 mg/mL against the tested bacteria, except for Staphyloccocus aureus and Escherichia coli. By contrast, higher inhibitory activity was seen against the tested fungi, especially against Bipolaris maydis. Following treatment with an MIC value of 250 μg/mL for 24 h, the mycelial morphology was markedly shriveled in appearance or showed flattened and empty hyphae, with fractured cell walls, ruptured plasmalemma and cytoplasmic coagulation or leakage. These structural changes hindered the growth of mycelia. View Full-Text
Keywords: tea seed pomace; saponin; LC-ESI-IT-TOF/MS; anti-fungal activity tea seed pomace; saponin; LC-ESI-IT-TOF/MS; anti-fungal activity
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MDPI and ACS Style

Zhang, X.-F.; Yang, S.-L.; Han, Y.-Y.; Zhao, L.; Lu, G.-L.; Xia, T.; Gao, L.-P. Qualitative and Quantitative Analysis of Triterpene Saponins from Tea Seed Pomace (Camellia oleifera Abel) and Their Activities against Bacteria and Fungi. Molecules 2014, 19, 7568-7580.

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