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Molecules 2014, 19(6), 7368-7387; https://doi.org/10.3390/molecules19067368

The Fruits of Wampee Inhibit H2O2-Induced Apoptosis in PC12 Cells via the NF-κB Pathway and Regulation of Cellular Redox Status

1
Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524023, Guangdong, China
2
Wuzhou Red Cross Hospital, Wuzhou 510663, Guangxi, China
3
Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, Guangdong, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 24 March 2014 / Revised: 26 May 2014 / Accepted: 27 May 2014 / Published: 5 June 2014
(This article belongs to the Section Natural Products Chemistry)
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Abstract

Wampee (Clausena lansium) fruits (CLS), whose pulp can be used to prepare fruit cups, desserts, jam, or jelly, can be eaten along with the peel. In this study, a PC12 cell model was built to observe the protective effect of CLS against H2O2-induced oxidative stress. We found that pretreatment with CLS increased cell viability and inhibited cytotoxicity, caspase-3 activity and DNA condensation. CLS also attenuated the increase in ROS production and MMP reduction. Moreover, we attempted to determine whether CLS suppressed the expression and phosphorylation of NF-κB. Western blot and immunostaining assay revealed that CLS inhibited H2O2-induced up-regulation of NF-κB p65 and pNF-κB p65. And CLS significantly suppressed the translocation of NF-κB p65 and pNF-κB p65 from cytoplasm to nuclear. Also, seven major compounds including a new flavanoid, luteolin-4'-O-β-d-gluco-pyranoside (3) and six known compounds 1,2, 47 were isolated and identified from CLS. Their antioxidative and H2O2-induced PC12 cell apoptosis-reversing activity were determined. These findings suggest that CLS and its major constituents (flavanoids) may be potential antioxidant agents and should encourage further research into their use as a functional food for neurodegenerative diseases. View Full-Text
Keywords: Clausena lansium; flavanoids; neurodegeneration; apoptosis; reactive oxygen species Clausena lansium; flavanoids; neurodegeneration; apoptosis; reactive oxygen species
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Zeng, X.; Zhou, X.; Cui, L.; Liu, D.; Wu, K.; Li, W.; Huang, R. The Fruits of Wampee Inhibit H2O2-Induced Apoptosis in PC12 Cells via the NF-κB Pathway and Regulation of Cellular Redox Status. Molecules 2014, 19, 7368-7387.

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