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Molecules 2014, 19(6), 7368-7387; doi:10.3390/molecules19067368

The Fruits of Wampee Inhibit H2O2-Induced Apoptosis in PC12 Cells via the NF-κB Pathway and Regulation of Cellular Redox Status

Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524023, Guangdong, China
Wuzhou Red Cross Hospital, Wuzhou 510663, Guangxi, China
Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, Guangdong, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Received: 24 March 2014 / Revised: 26 May 2014 / Accepted: 27 May 2014 / Published: 5 June 2014
(This article belongs to the Section Natural Products)
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Wampee (Clausena lansium) fruits (CLS), whose pulp can be used to prepare fruit cups, desserts, jam, or jelly, can be eaten along with the peel. In this study, a PC12 cell model was built to observe the protective effect of CLS against H2O2-induced oxidative stress. We found that pretreatment with CLS increased cell viability and inhibited cytotoxicity, caspase-3 activity and DNA condensation. CLS also attenuated the increase in ROS production and MMP reduction. Moreover, we attempted to determine whether CLS suppressed the expression and phosphorylation of NF-κB. Western blot and immunostaining assay revealed that CLS inhibited H2O2-induced up-regulation of NF-κB p65 and pNF-κB p65. And CLS significantly suppressed the translocation of NF-κB p65 and pNF-κB p65 from cytoplasm to nuclear. Also, seven major compounds including a new flavanoid, luteolin-4'-O-β-d-gluco-pyranoside (3) and six known compounds 1,2, 47 were isolated and identified from CLS. Their antioxidative and H2O2-induced PC12 cell apoptosis-reversing activity were determined. These findings suggest that CLS and its major constituents (flavanoids) may be potential antioxidant agents and should encourage further research into their use as a functional food for neurodegenerative diseases. View Full-Text
Keywords: Clausena lansium; flavanoids; neurodegeneration; apoptosis; reactive oxygen species Clausena lansium; flavanoids; neurodegeneration; apoptosis; reactive oxygen species

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zeng, X.; Zhou, X.; Cui, L.; Liu, D.; Wu, K.; Li, W.; Huang, R. The Fruits of Wampee Inhibit H2O2-Induced Apoptosis in PC12 Cells via the NF-κB Pathway and Regulation of Cellular Redox Status. Molecules 2014, 19, 7368-7387.

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