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Molecules 2014, 19(6), 7356-7367; doi:10.3390/molecules19067356

Oligo-Carrageenan Kappa-Induced Reducing Redox Status and Increase in TRR/TRX Activities Promote Activation and Reprogramming of Terpenoid Metabolism in Eucalyptus Trees

1
Marine Biotechnology Laboratory, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9170022, Chile
2
Molecular Magnetism Laboratory, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9170022, Chile
*
Author to whom correspondence should be addressed.
Received: 10 April 2014 / Revised: 30 May 2014 / Accepted: 30 May 2014 / Published: 5 June 2014
(This article belongs to the Section Natural Products)
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Abstract

In order to analyze whether the reducing redox status and activation of thioredoxin reductase (TRR)/thioredoxin(TRX) system induced by oligo-carrageenan (OC) kappa in Eucalyptus globulus activate secondary metabolism increasing terpenoid synthesis, trees were sprayed on the leaves with water, with OC kappa, or with inhibitors of NAD(P)H, ascorbate (ASC) and (GSH) synthesis and TRR activity, CHS-828, lycorine, buthionine sulfoximine (BSO) and auranofine, respectively, and with OC kappa and cultivated for four months. The main terpenoids in control Eucalyptus trees were eucalyptol (76%), α-pinene (7.4%), aromadendrene (3.6%), silvestrene (2.8%), sabinene (2%) and α-terpineol (0.9%). Treated trees showed a 22% increase in total essential oils as well as a decrease in eucalyptol (65%) and sabinene (0.8%) and an increase in aromadendrene (5%), silvestrene (7.8%) and other ten terpenoids. In addition, treated Eucalyptus showed seven de novo synthesized terpenoids corresponding to carene, α-terpinene, α-fenchene, γ-maaliene, spathulenol and α-camphenolic aldehyde. Most increased and de novo synthesized terpenoids have potential insecticidal and antimicrobial activities. Trees treated with CHS-828, lycorine, BSO and auranofine and with OC kappa showed an inhibition of increased and de novo synthesized terpenoids. Thus, OC kappa-induced reducing redox status and activation of TRR/TRX system enhance secondary metabolism increasing the synthesis of terpenoids and reprogramming of terpenoid metabolism in Eucalyptus trees. View Full-Text
Keywords: ascorbate; Eucalyptus globulus; glutathione; NADPH; oligo-carrageenan kappa; redox status; secondary metabolism; terpenoids; thioredoxin ascorbate; Eucalyptus globulus; glutathione; NADPH; oligo-carrageenan kappa; redox status; secondary metabolism; terpenoids; thioredoxin
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MDPI and ACS Style

González, A.; Gutiérrez-Cutiño, M.; Moenne, A. Oligo-Carrageenan Kappa-Induced Reducing Redox Status and Increase in TRR/TRX Activities Promote Activation and Reprogramming of Terpenoid Metabolism in Eucalyptus Trees. Molecules 2014, 19, 7356-7367.

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