Next Article in Journal
Surface-Functionalized Hyperbranched Poly(Amido Acid) Magnetic Nanocarriers for Covalent Immobilization of a Bacterial γ-Glutamyltranspeptidase
Previous Article in Journal
Microsomal Prostaglandin E Synthase-1 Deficiency Exacerbates Pulmonary Fibrosis Induced by Bleomycin in Mice
Molecules 2014, 19(4), 4986-4996; doi:10.3390/molecules19044986

High Efficiency Acetylcholinesterase Immobilization on DNA Aptamer Modified Surfaces

,  and *
Department of Chemistry, Imperial College, London SW7 2AZ, UK
* Author to whom correspondence should be addressed.
Received: 26 February 2014 / Revised: 8 April 2014 / Accepted: 11 April 2014 / Published: 21 April 2014
(This article belongs to the Section Organic Synthesis)
View Full-Text   |   Download PDF [629 KB, 18 June 2014; original version 18 June 2014]   |   Browse Figures


We report here the in vitro selection of DNA aptamers for electric eel acetylcholinesterase (AChE). One selected aptamer sequence (R15/19) has a high affinity towards the enzyme (Kd = 157 ± 42 pM). Characterization of the aptamer showed its binding is not affected by low ionic strength (~20 mM), however significant reduction in affinity occurred at high ionic strength (~1.2 M). In addition, this aptamer does not inhibit the catalytic activity of AChE that we exploit through immobilization of the DNA on a streptavidin-coated surface. Subsequent immobilization of AChE by the aptamer results in a 4-fold higher catalytic activity when compared to adsorption directly on to plastic.
Keywords: aptamers; acetylcholinesterase; immobilization aptamers; acetylcholinesterase; immobilization
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Chumphukam, O.; Le, T.T.; Cass, A.E.G. High Efficiency Acetylcholinesterase Immobilization on DNA Aptamer Modified Surfaces. Molecules 2014, 19, 4986-4996.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert