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Molecules 2014, 19(4), 4369-4379; doi:10.3390/molecules19044369
Article

Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS

1
,
2
,
1
,
2
 and
2,*
1 Laboratoire de Phytothérapie Appliquée aux Maladies Chroniques, Université Ferhat Abbas, Sétif 19000, Algeria 2 Laboratoire de Pharmacognosie, U.M.R./C.N.R.S. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, 4 Avenue de l'Observatoire, F-75006 Paris, France
* Author to whom correspondence should be addressed.
Received: 12 March 2014 / Revised: 29 March 2014 / Accepted: 1 April 2014 / Published: 9 April 2014
(This article belongs to the Section Natural Products)
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Abstract

Genista species are sources of antioxidant phenolic compounds such as O- and C-glycosylflavonoids and isoflavonoids. A combination of a DPPH scavenging assay with HPTLC-MS, a fast and efficient method for identification of bioactive compounds, has been applied for evaluation of the radical scavenging activity of metabolites from Genista saharae Coss. & Dur. Different organs collected at various periods have been compared. Identification of antioxidant compounds was obtained by elution of the major DPPH-inhibition zones. The resulting HPTLC-MS analysis under moderately polar conditions, coupled to the DPPH results led to the putative identification of two antioxidant isoflavone aglycones: 3',4',5,7-tetrahydroxyisoflavone (1) and ficuisoflavone (3), whereas polar migration conditions led to the identification of the glycosides 5-methoxy-4',7-trihydroxy-8-glucopyranosylisoflavone (4) and 4',5-dihydroxy-7-methoxyisoflavone-4'-O-β-D-gluco-pyranoside (5). Evaluation of percentage of inhibition of DPPH radical by the purified isoflavone 4 from the root extract showed that it affords a moderate contribution to the total radical scavenging activity of the extract.
Keywords: antioxidant; HPTLC-MS; Genista saharae; DPPH scavenging assay antioxidant; HPTLC-MS; Genista saharae; DPPH scavenging assay
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Meriane, D.; Genta-Jouve, G.; Kaabeche, M.; Michel, S.; Boutefnouchet, S. Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS. Molecules 2014, 19, 4369-4379.

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