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Molecules 2014, 19(4), 4369-4379; doi:10.3390/molecules19044369
Article

Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS

1
, 2
, 1
, 2
 and 2,*
1 Laboratoire de Phytothérapie Appliquée aux Maladies Chroniques, Université Ferhat Abbas, Sétif 19000, Algeria 2 Laboratoire de Pharmacognosie, U.M.R./C.N.R.S. 8638, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, 4 Avenue de l'Observatoire, F-75006 Paris, France
* Author to whom correspondence should be addressed.
Received: 12 March 2014 / Revised: 29 March 2014 / Accepted: 1 April 2014 / Published: 9 April 2014
(This article belongs to the Section Metabolites)
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Abstract

Genista species are sources of antioxidant phenolic compounds such as O- and C-glycosylflavonoids and isoflavonoids. A combination of a DPPH scavenging assay with HPTLC-MS, a fast and efficient method for identification of bioactive compounds, has been applied for evaluation of the radical scavenging activity of metabolites from Genista saharae Coss. & Dur. Different organs collected at various periods have been compared. Identification of antioxidant compounds was obtained by elution of the major DPPH-inhibition zones. The resulting HPTLC-MS analysis under moderately polar conditions, coupled to the DPPH results led to the putative identification of two antioxidant isoflavone aglycones: 3',4',5,7-tetrahydroxyisoflavone (1) and ficuisoflavone (3), whereas polar migration conditions led to the identification of the glycosides 5-methoxy-4',7-trihydroxy-8-glucopyranosylisoflavone (4) and 4',5-dihydroxy-7-methoxyisoflavone-4'-O-β-D-gluco-pyranoside (5). Evaluation of percentage of inhibition of DPPH radical by the purified isoflavone 4 from the root extract showed that it affords a moderate contribution to the total radical scavenging activity of the extract.
Keywords: antioxidant; HPTLC-MS; Genista saharae; DPPH scavenging assay antioxidant; HPTLC-MS; Genista saharae; DPPH scavenging assay
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Meriane, D.; Genta-Jouve, G.; Kaabeche, M.; Michel, S.; Boutefnouchet, S. Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS. Molecules 2014, 19, 4369-4379.

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