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Molecules 2014, 19(3), 3376-3388; doi:10.3390/molecules19033376
Article

Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption Process

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Received: 20 January 2014; in revised form: 6 March 2014 / Accepted: 6 March 2014 / Published: 19 March 2014
(This article belongs to the Special Issue Enzyme Immobilization)
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Abstract: The treatment of catechol via biocatalysis and adsorption with a commercial laccase immobilized on polyacrylonitrile/montmorillonite/graphene oxide (PAN/MMT/GO) composite nanofibers was evaluated with a homemade nanofibrous membrane reactor. The properties in this process of the immobilized laccase on PAN, PAN/MMT as well as PAN/MMT/GO with different weight ratios of MMT and GO were investigated. These membranes were successfully applied for removal of catechol from an aqueous solution. Scanning electron microscope images revealed different morphologies of the enzyme aggregates on different supports. After incorporation of MMT or MMT/GO, the optimum pH showed an alkaline shift to 4, compared to 3.5 for laccase immobilized on pure PAN nanofibers. The optimum temperature was at 55 °C for all the immobilized enzymes. Besides, the addition of GO improved the operational stability and storage stability. A 39% ± 2.23% chemical oxygen demand (COD) removal from the catechol aqueous solution was achieved. Experimental results suggested that laccase, PAN, adsorbent nanoparticles (MMT/GO) can be combined together for catechol treatment in industrial applications.
Keywords: laccase; enzyme immobilization; adsorbent; catechol; adsorption laccase; enzyme immobilization; adsorbent; catechol; adsorption
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, Q.; Cui, J.; Li, G.; Zhang, J.; Li, D.; Huang, F.; Wei, Q. Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption Process. Molecules 2014, 19, 3376-3388.

AMA Style

Wang Q, Cui J, Li G, Zhang J, Li D, Huang F, Wei Q. Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption Process. Molecules. 2014; 19(3):3376-3388.

Chicago/Turabian Style

Wang, Qingqing; Cui, Jing; Li, Guohui; Zhang, Jinning; Li, Dawei; Huang, Fenglin; Wei, Qufu. 2014. "Laccase Immobilized on a PAN/Adsorbents Composite Nanofibrous Membrane for Catechol Treatment by a Biocatalysis/Adsorption Process." Molecules 19, no. 3: 3376-3388.


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