Molecules 2014, 19(2), 1887-1911; doi:10.3390/molecules19021887
Article

Optimization of High-Pressure Ultrasonic-Assisted Simultaneous Extraction of Six Major Constituents from Ligusticum chuanxiong Rhizome using Response Surface Methodology

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Received: 19 December 2013; in revised form: 24 January 2014 / Accepted: 3 February 2014 / Published: 10 February 2014
(This article belongs to the Section Metabolites)
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Abstract: High-pressure ultrasound-assisted extraction technology was applied to extract ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A, ligustilide and levistolide A from Ligusticum chuanxiong rhizomes. Seven independent variables, including solvent type, pressure, particle size, liquid-to-solid ratio, extraction temperature, ultrasound power, and extraction time were examined. Response Surface Methodology (RSM) using a Central Composite Design (CCD) was employed to optimize the experimental conditions (extraction temperature, ultrasonic power, and extraction time) on the basis of the results of single factor tests for the extraction of these six major components in L. chuanxiong rhizomes. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and were also examined using appropriate statistical methods. The best extraction conditions were as follows: extraction solvent: 40% ethanol; pressure: 10 MPa; particle size: 80 mesh; liquid-to-solid ratio: 100:1; extraction temperature: 70 °C; ultrasonic power, 180 W; and extraction time, 74 min.
Keywords: Ligusticum chuanxiong; major constituents; response surface methodology; optimization; high-pressure ultrasonic-assisted extraction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Liu, J.-L.; Zheng, S.-L.; Fan, Q.-J.; Yuan, J.-C.; Yang, S.-M.; Kong, F.-L. Optimization of High-Pressure Ultrasonic-Assisted Simultaneous Extraction of Six Major Constituents from Ligusticum chuanxiong Rhizome using Response Surface Methodology. Molecules 2014, 19, 1887-1911.

AMA Style

Liu J-L, Zheng S-L, Fan Q-J, Yuan J-C, Yang S-M, Kong F-L. Optimization of High-Pressure Ultrasonic-Assisted Simultaneous Extraction of Six Major Constituents from Ligusticum chuanxiong Rhizome using Response Surface Methodology. Molecules. 2014; 19(2):1887-1911.

Chicago/Turabian Style

Liu, Jin-Liang; Zheng, Shun-Lin; Fan, Qiao-Jia; Yuan, Ji-Chao; Yang, Shi-Min; Kong, Fan-Lei. 2014. "Optimization of High-Pressure Ultrasonic-Assisted Simultaneous Extraction of Six Major Constituents from Ligusticum chuanxiong Rhizome using Response Surface Methodology." Molecules 19, no. 2: 1887-1911.


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