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Molecules 2014, 19(2), 1422-1431; doi:10.3390/molecules19021422

Griffipavixanthone from Garcinia oblongifolia Champ Induces Cell Apoptosis in Human Non-Small-Cell Lung Cancer H520 Cells in Vitro

1
School of Life Sciences, South China Normal University, Guangzhou 510631, China
2
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
3
Laboratory of Southern Subtropical Plant Diversity, Shenzhen Fairy Lake Botanical Garden, Chinese Academy of Sciences, Shenzhen 518004, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 9 December 2013 / Revised: 14 January 2014 / Accepted: 18 January 2014 / Published: 27 January 2014
(This article belongs to the Section Natural Products)
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Abstract

Griffipavixanthone (GPX) is a dimeric xanthone which was isolated in a systematic investigation of Garcinia oblongifolia Champ. In this study, we investigate the effect of GPX on cell proliferation and apoptosis on human Non-small-cell lung cancer (NSCLC) cells in vitro and determine the mechanisms of its action. GPX inhibited the growth of H520 cells in dose- and time-dependent manners, with IC50 values of 3.03 ± 0.21 μM at 48 h. The morphologic characteristics of apoptosis and apoptotic bodies were observed by fluorescence microscope and transmission electron microscope. In addition, Annexin V/PI double staining assay revealed that cells in early stage of apoptosis were significantly increased upon GPX treatment dose-dependently. Rh123 staining assay indicated that GPX reduced the mitochondrial membrane potential. DCFH-DA staining revealed that intracellular ROS increased with GPX treatment. Moreover, GPX cleaved and activated caspase-3. In summary, this study showed that GPX inhibited H520 cell proliferation in dose- and time-dependent manner. Further mechanistic study indicated that GPX induced cell apoptosis through mitochondrial apoptotic pathway accompanying with ROS production. Our results demonstrate the potential application of GPX as an anti-non-small cell lung cancer agent.
Keywords: griffipavixanthone; H520 cells; apoptosis; caspase; mitochondrial transmembrane potential; ROS generation griffipavixanthone; H520 cells; apoptosis; caspase; mitochondrial transmembrane potential; ROS generation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Shi, J.-M.; Huang, H.-J.; Qiu, S.-X.; Feng, S.-X.; Li, X.-E. Griffipavixanthone from Garcinia oblongifolia Champ Induces Cell Apoptosis in Human Non-Small-Cell Lung Cancer H520 Cells in Vitro. Molecules 2014, 19, 1422-1431.

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