Molecules 2014, 19(2), 1422-1431; doi:10.3390/molecules19021422
Article

Griffipavixanthone from Garcinia oblongifolia Champ Induces Cell Apoptosis in Human Non-Small-Cell Lung Cancer H520 Cells in Vitro

1,†email, 1,†email, 2email, 3,* email and 1,* email
Received: 9 December 2013; in revised form: 14 January 2014 / Accepted: 18 January 2014 / Published: 27 January 2014
(This article belongs to the Section Natural Products)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Griffipavixanthone (GPX) is a dimeric xanthone which was isolated in a systematic investigation of Garcinia oblongifolia Champ. In this study, we investigate the effect of GPX on cell proliferation and apoptosis on human Non-small-cell lung cancer (NSCLC) cells in vitro and determine the mechanisms of its action. GPX inhibited the growth of H520 cells in dose- and time-dependent manners, with IC50 values of 3.03 ± 0.21 μM at 48 h. The morphologic characteristics of apoptosis and apoptotic bodies were observed by fluorescence microscope and transmission electron microscope. In addition, Annexin V/PI double staining assay revealed that cells in early stage of apoptosis were significantly increased upon GPX treatment dose-dependently. Rh123 staining assay indicated that GPX reduced the mitochondrial membrane potential. DCFH-DA staining revealed that intracellular ROS increased with GPX treatment. Moreover, GPX cleaved and activated caspase-3. In summary, this study showed that GPX inhibited H520 cell proliferation in dose- and time-dependent manner. Further mechanistic study indicated that GPX induced cell apoptosis through mitochondrial apoptotic pathway accompanying with ROS production. Our results demonstrate the potential application of GPX as an anti-non-small cell lung cancer agent.
Keywords: griffipavixanthone; H520 cells; apoptosis; caspase; mitochondrial transmembrane potential; ROS generation
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MDPI and ACS Style

Shi, J.-M.; Huang, H.-J.; Qiu, S.-X.; Feng, S.-X.; Li, X.-E. Griffipavixanthone from Garcinia oblongifolia Champ Induces Cell Apoptosis in Human Non-Small-Cell Lung Cancer H520 Cells in Vitro. Molecules 2014, 19, 1422-1431.

AMA Style

Shi J-M, Huang H-J, Qiu S-X, Feng S-X, Li X-E. Griffipavixanthone from Garcinia oblongifolia Champ Induces Cell Apoptosis in Human Non-Small-Cell Lung Cancer H520 Cells in Vitro. Molecules. 2014; 19(2):1422-1431.

Chicago/Turabian Style

Shi, Jun-Min; Huang, Hui-Juan; Qiu, Sheng-Xiang; Feng, Shi-Xiu; Li, Xu-E. 2014. "Griffipavixanthone from Garcinia oblongifolia Champ Induces Cell Apoptosis in Human Non-Small-Cell Lung Cancer H520 Cells in Vitro." Molecules 19, no. 2: 1422-1431.

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