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Molecules 2014, 19(2), 1378-1393; doi:10.3390/molecules19021378
Published: 27 January 2014
Abstract: Activity-based protein profiling (ABPP) is a chemical proteomic technique that enables the interrogation of protein activity directly within complex proteomes. Given the dominant role of posttranslational modifications in regulating protein function in vivo, ABPP provides a direct readout of activity that is not attained through traditional proteomic methods. ABPP relies on the design of covalent binding probes that either target a specific enzyme or a class of enzymes with related function. These covalent warheads are coupled to either fluorophores or biotin groups for visualization and enrichment of these active proteins. The advent of bioorthogonal chemistries, in particular, the copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC), has benefitted the field of ABPP by achieving the following: (1) replacing bulky reporter groups with smaller alkyne or azide groups to promote cell permeability; (2) adding modularity to the system such that a single probe can be diversified with a variety of reporter groups without the need to develop new synthetic routes; and (3) enabling the conjugation of complex linkers to facilitate quantitative proteomic analyses. Here, we summarize recent examples of CuAAC in ABPP that serve to illustrate the contribution of bioorthogonal chemistry to advancing discoveries in this field.
The ability to directly monitor protein activity within a native biological system is essential to understanding the dysregulation of proteins in disease. Standard proteomic methods such as two-dimensional gel electrophoresis and mass spectrometry can effectively quantify protein abundance in complex proteomes . However, due to the diverse and dynamic posttranslational modifications that regulate protein activity in vivo , these methods do not afford insight into the fraction of active protein within a given system. To address these limitations, the field of activity-based protein profiling (ABPP) was developed to complement existing genomic and proteomic technologies [3,4,5,6]. ABPP relies on the use of chemical probes that selectively react with the active site of a specific protein, or a class of proteins with shared functional properties . These activity-based probes (ABPs) are typically composed of a reactive electrophile to covalently modify an active-site residue, and a reporter group, such as a fluorophore for visualization by in-gel fluorescence, or a biotin for both western blot analysis and avidin enrichment prior to mass-spectrometry (MS)-based identification (Figure 1A) [8,9].
While useful for in vitro analysis of protein activities, these bulky reporter groups can hinder cellular uptake and protein affinity when administered in vivo. To circumvent these issues, ABPs can be functionalized instead with sterically inconspicuous bio-orthogonal handles to facilitate attachment of reporter groups ex vivo (Figure 1B) [10,11,12,13]. An ideal bioorthogonal reaction involves the rapid and selective coupling of two biologically inert coupling partners under physiological conditions . The Staudinger ligation was one of the first bioorthogonal reactions to be developed, and is based on the modified Staudinger reaction between azides and triarylphosphines (Figure 2A) [15,16]. More recently, the tetrazine ligation has found wide utility and couples a highly strained trans-cyclooctene to functionalized tetrazines (Figure 2B) [17,18,19]. The most widely used click chemistry reaction is the copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) between an azide and a terminal alkyne to generate a 1,4-disubstituted 1,2,3-triazole (Figure 2C) [20,21]. Concerns about the use of a cytotoxic copper species to catalyze the reaction promoted the development of a copper-free variant of this reaction that utilizes a strained alkyne to accelerate the reaction (Figure 2D) [22,23,24]. Furthermore, the development of ligands that simultaneously act to reduce reactive oxygen species produced during typical CuAAC reactions further ameliorate toxicity issues associated with this reaction and enable live-cell labeling . All of these bioorthogonal reactions have been applied to the field of ABPP and many of these studies have been reviewed previously . Here we will specifically focus on the application of the CuAAC bioorthogonal reaction to ABPP. CuAAC has advanced the field of ABPP by expanding the enzyme classes that can be targeted by ABPs, enabling cellular and in vivo studies and providing technological platforms to quantitatively monitor protein activities in complex biological systems.
2. The Development of CuAAC and Early Applications to ABPP
The CuAAC reaction is a derivative of the Huisgen 1,3-dipolar cycloaddition  that fuses azides and alkynes to form triazoles (Figure 2C). This reaction is ideal for biological applications due to the high stability of azides to water, ambient oxygen and a wide-variety of synthetic transformations . The synthetic ease of incorporating azides and alkynes into biological probes, coupled with the minimal steric disruption caused by these functionalities, has further promoted the use of CuAAC in biological applications. The initial Huisgen cycloaddition required high temperatures and formed a mixture of 1,4- and 1,5-triazole regioisomers, but addition of a copper(I) catalyst provides exclusively 1,4-disubstituted-1,2,3-triazoles at room temperature, at a wide range of pH values, and in high yield [20,27]. The biocompatibility of this reaction was originally demonstrated through decoration of viral capsids . The CuAAC reaction was first utilized in the field of ABPP to couple an azide-derivatized phenyl sulfonate ester ABP (PS-N3) to an alkyne-bearing rhodamine moiety (Rh-≡) . The PS-N3 probe labeled GSTO 1-1 proteins in cell lysates more efficiently than the standard rhodamine-tagged phenyl sulfonate probe (PS-Rh). Furthermore, PS-N3 was shown to facilitate in vivo ABPP, as cells and animals treated with PS-N3 showed robust protein labeling upon administration of the CuAAC reagents ex vivo . Further optimization of this platform revealed that the use of rhodamine-azide (Rh-N3) greatly reduced the high background labeling of proteins that was observed with Rh-≡, although with lower kinetics of labeling . This initial foray into “tag-free” ABPP clearly demonstrated the key advantages of this platform, which includes better distribution of the probe in cells and animals, improved access to protein active sites, and streamlined probe synthesis to create a single modular probe that can be linked to a variety of reporter groups. Since then, “tag-free” ABPP has been employed in a variety of studies that have resulted in the expansion of ABPP into new enzyme classes.
3. Alkyne-Tagged ABPs for the Serine Hydrolase Family
The first class of enzymes targeted for ABPP studies were the serine hydrolases (SHs), which comprise a large and diverse family of enzymes that perform numerous roles in physiological (e.g., blood coagulation, inflammation, angiogenesis) and pathological (e.g., emphysema, cancer) processes . This family of enzymes is characterized by an active site serine residue that is rendered nucleophilic by the presence of a catalytic dyad or triad involving proximal Lys, Asp and His residues . ABPs for this family of enzymes were derived from fluorophosphonates (FPs), which were known to be mechanism-based inhibitors that mimic the enzyme-substrate tetrahedral intermediate and covalently trap the active site serine [30,31]. Most ABPP studies for SHs are performed using biotin or rhodamine tagged FP , but CuAAC has shown utility in the profiling of these enzymes with the generation of a “tag-free” FP-alkyne (FP-≡) (Figure 3A) [33,34]. This probe was shown to label serine proteases with greater affinity than the reporter-tag functionalized FP probes . More importantly, the vastly improved cell permeability of FP-≡ facilitates profiling of SH activity directly in living cells. This improved cell permeability enabled the development of a competitive ABPP platform to screen inhibitor selectivity directly within a cellular milieu instead of in lysates where information pertaining to subcellular localization is disrupted . In addition to pan-serine hydrolase ABPs, FP-based probes for specific SHs have been developed with inclusion of an alkyne handle for CuAAC. These include phosphatidylcholine-based probes where the reactive FP-warhead is positioned at either sn-1 or sn-2 positions (Figure 3B). These probes were shown to label functional subclasses of serine phospholipases . Unlike bulky biotin or rhodamine reporter groups, the alkyne causes minimal steric perturbation and better mimics the acyl chain in the endogenous substrates for these phospholipases.
Electrophilic groups other than the fluorophosphonate can also be used to covalently modify the active-site serine of SHs. These other serine-reactive electrophiles include nitrophenyl-phosphonate esters (NP), acyloxymethylketones (AOMK) and carbamates. Many ABPs containing these electrophiles have been functionalized with azides or alkynes for “tag-free” ABPP using CuAAC. The NP and AOMK reactive groups have been incorporated into alkyne-functionalized fatty acid-based chemical probes for profiling lipid-associated proteins (Figure 3C,D) [35,36]. The minimal steric encumbrance of the alkyne facilitates cellular uptake and enables activity-based profiling of a variety of fatty acid-modifying proteins in living cells [35,36]. The NP fatty acid probes were additionally used to image the subcellular localization of active lipases. This was achieved by performing CuAAC to incorporate a fluorophore for protein visualization after fixation of the cells (Figure 3E) . In a similar imaging study, an alkyne-functionalized analog of Orlistat, a β-lactone-based covalent inhibitor of fatty acid synthase (FASN), was used to image the localization of FASN during Hepatitis C Virus infection (Figure 3F) . The SH-reactive carbamate reactive group was incorporated into a library of alkyne-functionalized small molecules (Figure 3G) and led to the discovery of a hexafluoroisopropyl carbamate that specifically labels two endocannabinoid hydrolases (monoacylglycerol lipase (MAGL) and α, β hydrolase-6 (ABHD6)) in cells .
In addition to the lipases, serine proteases have also been targeted by “tag-free” ABPP. Pan serine protease ABPs for in-cell labeling have been developed from the irreversible inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF) , iso-coumarins that target subclasses of serine proteases , peptidyl aldehydes and phosphonates that specifically label prolyl oligopeptidase [41,42], and β-lactones that inhibit rhomboid proteases .
4. CuAAC-Compatible Covalent Probes for Other Enzyme Families
Although the serine hydrolases were the subject of some of the earliest ABPP studies, “tag-free” ABPs are constantly being developed for diverse enzyme families. As with the SH probes, the use of CuAAC in each of these cases improves protein reactivity and cell permeability. One family of enzymes recently targeted by CuAAC-mediated ABPP facilitates studies into the ubiquitin and ubiquitin-like protein (UBL) signaling pathways . A series of ubiquitin-conjugating enzymes tag proteins with ubiquitin for targeted degradation by the proteasome , including UBL activating enzymes (UBE1), UBL conjugating enzymes (UBE2), and UBL ligases (UBE3). Since UBE1 enzymes dictate the activity of the entire ubiquitin-signaling pathway, a click-chemistry compatible ABP for UBE1 enzymes was developed to measure UBE1 activity inside cells . The UBE1 ABP (termed ABP1; Figure 4A) was designed to mimic and bind the AMP binding site of the UBE1-UBL thioester complex, positioning a nucleophilic sulfamate group for attack of the UBE1-UBL thioester to form a covalent adduct. This cell permeable ABP1 efficiently labels active UBE1 enzymes in numerous cancer cell lines, allowing for profiling the selectivity of UBE1 inhibitors within cells, and facilitating the discovery of previously unknown UBL proteins .
The epoxide-containing natural product E-64 has been shown to potently inhibit several papain-like cysteine proteases by alkylating the active site cysteine [46,47]. A set of E-64 derivatives functionalized with azide (N3Le) or alkyne (≡Le) handles were generated for in vivo labeling of plant tissues . In contrast to biotin-tagged E-64 , N3Le and ≡Le efficiently labeled proteases in living plant tissues . In this same study, a similar approach was utilized to label the proteasome in plants. Several ABPs for the proteasome have been developed for bioorthogonal reactions outside of CuAAC [13,49,50,51] One of those proteasome ABPs was adapted for CuAAC by incorporation of an azide functional group for CuAAC-mediated coupling to biotin after treatment of living plant tissues. These studies enabled the screening of inhibitors for selectivity in plant tissues .
Outside of the cysteine proteases and ubiquitinating enzymes, several other enzymes with nucleophilic active-site cysteines have been targeted for CuAAC-mediated ABPP. One such family is the protein arginine deiminases (PADs), which catalyze the conversion of arginine residues to citrulline in the presence of calcium . Several members of the PAD family have been shown to play critical roles in cell signaling pathways, including apoptosis and cell differentiation, as well as transcriptional regulation by modifying arginine residues in the tails of histones . PAD probes were constructed around mechanism-based inactivators that contain a haloacetamidine (Cl or F) to covalently modify the active site cysteine . Alkyne-functionalized PAD ABPs (Figure 4B) were shown to be more selective and efficient at labeling PAD4 in cells compared to the biotin and fluorescein-tagged counterparts . This increased efficiency of labeling is attributed to the rapid degradation of PAD4 in cell lysates, which is circumvented by in-cell labeling with CuAAC-compatible probes. A similar alkyne-functionalized chloroacetamidine probe (Figure 4B) was shown to label the related enzyme dimethylarginine dimethylaminohydrolase (DDAH) . Similar to the PADs, DDAH activity is diminished in lysates due to inactivation by a variety of effector molecules, therefore in vivo labeling using CuAAC-compatible probes serves to preserve enzyme activity .
Cytochrome P450 enzymes are central to the metabolism of numerous drugs, xenobiotics, and endogenous metabolites, and understanding the subset of active P450 enzymes is essential to the drug development process . A set of ABPs based on three different mechanism-based inhibitors, containing aryl alkynes (Figure 4C), propynyl groups, or furanocoumarin, was synthesized and demonstrated complementarity in profiling the entire family of human P450s . This panel of P450 APBs enabled investigation into the effects of clinically used aromatase inhibitors on P450 activity . The sterically inconspicuous alkyne was critical to these studies, as biotin or fluorophore-tagged probes are less similar to typical drug-molecules that are metabolized by the P450s and therefore are not substrates for these enzymes.
Lastly, CuAAC-mediated ABPP has been extended to profile members of the glycosidase family. The exoglycosidase neuraminidase (NA; also known as sialidase) is produced by many pathogens to facilitate invasion and immunological escape . A 3-fluorosialyl fluoride (DFSA) probe (Figure 4D) was designed to specifically label NA as well as an ester-protected version (PDFSA) to enhance cell permeability and label NA in vivo . Imaging of active NA in influenza virus-infected cells was accomplished by CuAAC-mediated conjugation of biotin-azide followed by staining with DyLight488-streptavidin. The NA inhibitor Oseltamivir (OS) competitively inhibits DFSA binding, yet OS-resistant strains such as H1N1 are able to bind DSFA even in the presence of OS, suggesting the application of the DFSA probe for detection of OS-resistant influenza strains .
6. Applications of CuAAC in Mass Spectrometry-Based ABPP
SDS-PAGE and in-gel fluorescence only reveal a fraction of proteins targeted by APBs, and therefore, many mass spectrometry (MS)-based methods have emerged as gel-free alternatives that provide higher resolution and greater sensitivity . One of the advantages of CuAAC-based ABPP methods is the ability to conjugate probe-labeled proteins to a variety of linkers that enable a diverse array of mass-spectrometry analyses. Typical MS-ABPP strategies utilize CuAAC to couple a biotin-azide for subsequent enrichment and identification of labeled proteins by mass spectrometry . More advanced MS experiments involve the installment of a chemically or proteolytically cleavable linker that further allows for identification of the site of probe labeling, as well as the relative quantification of labeled proteins from two or more biological samples. A platform titled tandem orthogonal proteolysis (TOP)-ABPP utilizes CuAAC to append a linker comprised of a biotin group and an azide, separated by the recognition sequence for the tobacco-etch virus (TEV) protease (Figure 6A) [73,74].
Upon enrichment of probe-labeled proteins, on-bead trypsin digestion releases all but the probe-labeled peptides, which are selectively released from the beads by treatment with the TEV protease. MS analysis of this peptide mixture identifies the exact site of probe labeling from a complex proteome. This platform has been further modified by incorporating an isotopic label into the TEV linker, affording isotopically light and heavy variants that could be utilized to measure the extent of probe labeling across two different proteomes . A further modification included the replacement of the protease-cleavable linker with a chemically cleavable azobenzene, allowing for faster cleavage of labeled peptides from the beads . These platforms have expanded the scope and utility of ABPP through applications evaluating the amino-acid specificity of electrophiles used for ABPP , ranking cysteine residues in the proteome by their nucleophilicity so as to globally identify functional cysteines , and identifying residues subject to post-translational modification through reactive lipid species  and metal binding .
The utility of CuAAC in ABPP is further illustrated in a study that utilizes imaging mass spectrometry to study the distribution of active serine hydrolases in tissue . In this study, tissue sections were treated with FP-≡, followed by incorporation of a mass-tagged dendrimer using CuAAC. The mass tags are connected to the dendrimer via laser-cleavable linkers (Figure 6B), thereby releasing the mass tags upon laser irradiation for direct analysis by MALDI-MS . CuAAC has also been applied to quantification of serine protease activity using ICP/MS . In this study, alkyne functionalized AEBSF analogs were modified with a europium-loaded azido-monoamide-DOTA using CuAAC (Figure 6C). Serine proteases that reacted with this probe were analyzed by ICP/MS allowing for absolute quantification of active serine proteases in a mixture .
CuAAC methods have greatly simplified these applications by circumventing the need to synthesize every ABP with the complex cleavable linkers pre-attached. The advent of CuAAC has resulted in a vastly modular process, where a single ABP can be evaluated through a variety of techniques by appending different tags using CuAAC.
ABPP allows for selective labeling, visualization, and enrichment of active enzymes in a complex proteome. Initial ABPP probes were comprised of a reactive group directly conjugated to a reporter element such as a fluorophore or biotin. These bulky reporter groups precluded the application of ABPP to enzymes with binding sites that were unable to accommodate the reporter, and furthermore limited the use of ABP labeling in live cells and organisms due to the reduced cell permeability of the probe. Upon the advent of bioorthogonal chemistry, several mild and bio-compatible reactions were generated that enabled conjugation of the reporter element ex vivo after the initial probe-labeling was performed in vivo. Of these various bioorthogonal reactions, we focused specifically on CuAAC and its application to ABPP. CuAAC has significantly modularized ABPP approaches, whereby a single ABP can now be conjugated to a variety of reporter elements depending on the application of interest. Furthermore, CuAAC compatible probes are much smaller than reporter-tagged probes, facilitating probe labeling within the unperturbed environment of a live cell or animal. As emphasized in this review, many protein activities are modulated by protein complexes and other in vivo factors that are disrupted by homogenization, thereby CuAAC has greatly increased the number of enzyme families accessible to this technique. Furthermore, the small size of these clickable probes causes minimal steric interaction with enzyme active sites, thereby improving binding affinity and enhancing labeling. Lastly, the advent of CuAAC has facilitated the expansion of ABPP by coupling to advanced analytical platforms such as MALDI-imaging of active enzyme in tissue slices, absolute quantitation of active enzyme by ICP-MS, and direct identification and quantitation of the exact sites of probe labeling. Further advances in the efficiency of CuAAC, and the development of diverse clickable linkers to unite ABPP with a wide array of analytical techniques, will serve to expand the biological insight gained through this chemical proteomic technique.
Conflicts of Interest
The authors declare no conflict of interest.
- Zhang, Y.; Fonslow, B.R.; Shan, B.; Baek, M.C.; Yates, J.R., 3rd. Protein analysis by shotgun/bottom-up proteomics. Chem. Rev. 2013, 113, 2343–2394, doi:10.1021/cr3003533.
- Kobe, B.; Kemp, B.E. Active site-directed protein regulation. Nature 1999, 402, 373–376, doi:10.1038/46478.
- Adam, G.C.; Sorensen, E.J.; Cravatt, B.F. Chemical strategies for functional proteomics. Mol. Cell. Proteomics 2002, 1, 781–790, doi:10.1074/mcp.R200006-MCP200.
- Cravatt, B.F.; Sorensen, E.J. Chemical strategies for the global analysis of protein function. Curr. Opin. Chem. Biol. 2000, 4, 663–668, doi:10.1016/S1367-5931(00)00147-2.
- Sadaghiani, A.M.; Verhelst, S.H.; Bogyo, M. Tagging and detection strategies for activity-based proteomics. Curr. Opin. Chem. Biol. 2007, 11, 20–28, doi:10.1016/j.cbpa.2006.11.030.
- Fonovic, M.; Bogyo, M. Activity-based probes as a tool for functional proteomic analysis of proteases. Expert Rev. Proteomics 2008, 5, 721–730, doi:10.1586/147894188.8.131.521.
- Evans, M.J.; Cravatt, B.F. Mechanism-based profiling of enzyme families. Chem. Rev. 2006, 106, 3279–3301, doi:10.1021/cr050288g.
- Cravatt, B.F.; Wright, A.T.; Kozarich, J.W. Activity-based protein profiling: From enzyme chemistry. Annu. Rev. Biochem. 2008, 77, 383–414, doi:10.1146/annurev.biochem.75.101304.124125.
- Speers, A.E.; Cravatt, B.F. Chemical strategies for activity-based proteomics. Chembiochem 2004, 5, 41–47, doi:10.1002/cbic.200300721.
- Speers, A.E.; Adam, G.C.; Cravatt, B.F. Activity-based protein profiling in vivo using a copper(i)-catalyzed azide-alkyne [3 + 2] cycloaddition. J. Am. Chem. Soc. 2003, 125, 4686–4687, doi:10.1021/ja034490h.
- Speers, A.E.; Cravatt, B.F. Profiling enzyme activities in vivo using click chemistry methods. Chem. Biol. 2004, 11, 535–546, doi:10.1016/j.chembiol.2004.03.012.
- Speers, A.E.; Cravatt, B.F. Activity-based protein profiling (ABPP) and click chemistry (CC)-ABPP by MudPIT mass spectrometry. Curr. Protoc. Chem. Biol. 2009, 1, 29–41.
- Willems, L.I.; van der Linden, W.A.; Li, N.; Li, K.Y.; Liu, N.; Hoogendoorn, S.; van der Marel, G.A.; Florea, B.I.; Overkleeft, H.S. Bioorthogonal chemistry: Applications in activity-based protein profiling. Acc. Chem. Res. 2011, 44, 718–729, doi:10.1021/ar200125k.
- Ramil, C.P.; Lin, Q. Bioorthogonal chemistry: Strategies and recent developments. Chem. Commun. 2013, 49, 11007–11022, doi:10.1039/c3cc44272a.
- Saxon, E.; Armstrong, J.I.; Bertozzi, C.R. A “traceless” Staudinger ligation for the chemoselective synthesis of amide bonds. Org. Lett. 2000, 2, 2141–2143, doi:10.1021/ol006054v.
- Saxon, E.; Bertozzi, C.R. Cell surface engineering by a modified Staudinger reaction. Science 2000, 287, 2007–1010, doi:10.1126/science.287.5460.2007.
- Devaraj, N.K.; Weissleder, R. Biomedical applications of tetrazine cycloadditions. Acc. Chem. Res. 2011, 44, 816–827, doi:10.1021/ar200037t.
- Devaraj, N.K.; Upadhyay, R.; Haun, J.B.; Hilderbrand, S.A.; Weissleder, R. Fast and sensitive pretargeted labeling of cancer cells through a tetrazine/trans-cyclooctene cycloaddition. Angew. Chem. Int. Ed. Engl. 2009, 48, 7013–7016, doi:10.1002/anie.200903233.
- Devaraj, N.K.; Weissleder, R.; Hilderbrand, S.A. Tetrazine-based cycloadditions: Application to pretargeted live cell imaging. Bioconjug. Chem. 2008, 19, 2297–2299, doi:10.1021/bc8004446.
- Tornoe, C.W.; Christensen, C.; Meldal, M. Peptidotriazoles on solid phase: [1,2,3]-triazoles by regiospecific copper(i)-catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides. J. Org. Chem. 2002, 67, 3057–3064, doi:10.1021/jo011148j.
- Wang, Q.; Chan, T.R.; Hilgraf, R.; Fokin, V.V.; Sharpless, K.B.; Finn, M.G. Bioconjugation by copper(I)-catalyzed azide-alkyne [3 + 2] cycloaddition. J. Am. Chem. Soc. 2003, 125, 3192–3193, doi:10.1021/ja021381e.
- Chang, P.V.; Prescher, J.A.; Sletten, E.M.; Baskin, J.M.; Miller, I.A.; Agard, N.J.; Lo, A.; Bertozzi, C.R. Copper-free click chemistry in living animals. Proc. Natl. Acad. Sci. USA 2010, 107, 1821–1826, doi:10.1073/pnas.0911116107.
- Baskin, J.M.; Prescher, J.A.; Laughlin, S.T.; Agard, N.J.; Chang, P.V.; Miller, I.A.; Lo, A.; Codelli, J.A.; Bertozzi, C.R. Copper-free click chemistry for dynamic in vivo imaging. Proc. Natl. Acad. Sci. USA 2007, 104, 16793–16797, doi:10.1073/pnas.0707090104.
- Agard, N.J.; Prescher, J.A.; Bertozzi, C.R. A strain-promoted [3 + 2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems. J. Am. Chem. Soc. 2004, 126, 15046–15047, doi:10.1021/ja044996f.
- Hong, V.; Steinmetz, N.F.; Manchester, M.; Finn, M.G. Labeling live cells by copper-catalyzed alkyne–azide click chemistry. Bioconjug. Chem. 2010, 21, 1912–1916, doi:10.1021/bc100272z.
- Huisgen, R. 1,3,-Dipolar Cycloaddition Chemistry; Wiley: New York, NY, USA, 1984; pp. 1–176.
- Rostovtsev, V.V.; Green, L.G.; Fokin, V.V.; Sharpless, K.B. A stepwise huisgen cycloaddition process: Copper(I)-catalyzed regioselective “ligation” of azides and terminal alkynes. Angew. Chem. Int. Ed. Engl. 2002, 41, 2596–2599, doi:10.1002/1521-3773(20020715)41:14<2596::AID-ANIE2596>3.0.CO;2-4.
- Long, J.Z.; Cravatt, B.F. The metabolic serine hydrolases and their functions in mammalian physiology and disease. Chem. Rev. 2011, 111, 6022–6063, doi:10.1021/cr200075y.
- Dodson, G.; Wlodawer, A. Catalytic triads and their relatives. Trends Biochem. Sci. 1998, 23, 347–352, doi:10.1016/S0968-0004(98)01254-7.
- Kidd, D.; Liu, Y.; Cravatt, B.F. Profiling serine hydrolase activities in complex proteomes. Biochemistry 2001, 40, 4005–4015, doi:10.1021/bi002579j.
- Liu, Y.; Patricelli, M.P.; Cravatt, B.F. Activity-based protein profiling: The serine hydrolases. Proc. Natl. Acad. Sci. USA 1999, 96, 14694–14699, doi:10.1073/pnas.96.26.14694.
- Simon, G.M.; Cravatt, B.F. Activity-based proteomics of enzyme superfamilies: Serine hydrolases as a case study. J. Biol. Chem. 2010, 285, 11051–11055, doi:10.1074/jbc.R109.097600.
- Gillet, L.C.; Namoto, K.; Ruchti, A.; Hoving, S.; Boesch, D.; Inverardi, B.; Mueller, D.; Coulot, M.; Schindler, P.; Schweigler, P.; et al. In-cell selectivity profiling of serine protease inhibitors by activity-based proteomics. Mol. Cell. Proteomics 2008, 7, 1241–1253, doi:10.1074/mcp.M700505-MCP200.
- Tully, S.E.; Cravatt, B.F. Activity-based probes that target functional subclasses of phospholipases in proteomes. J. Am. Chem. Soc. 2010, 132, 3264–3265, doi:10.1021/ja1000505.
- Raghavan, A.; Charron, G.; Flexner, J.; Hang, H.C. Chemical probes for profiling fatty acid-associated proteins in living cells. Bioorg. Med. Chem. Lett. 2008, 18, 5982–5986, doi:10.1016/j.bmcl.2008.09.083.
- Viertler, M.; Schittmayer, M.; Birner-Gruenberger, R. Activity based subcellular resolution imaging of lipases. Bioorg. Med. Chem. 2012, 20, 628–632, doi:10.1016/j.bmc.2011.04.018.
- Nasheri, N.; Joyce, M.; Rouleau, Y.; Yang, P.; Yao, S.; Tyrrell, D.L.; Pezacki, J.P. Modulation of fatty acid synthase enzyme activity and expression during hepatitis C virus replication. Chem. Biol. 2013, 20, 570–582, doi:10.1016/j.chembiol.2013.03.014.
- Chang, J.W.; Cognetta, A.B.; Niphakis, M.J.; Cravatt, B.F. Proteome-wide reactivity profiling identifies diverse carbamate chemotypes tuned for serine hydrolase inhibition. ACS Chem. Biol. 2013, 8, 1590–1599, doi:10.1021/cb400261h.
- Shannon, D.A.; Gu, C.; McLaughlin, C.J.; Kaiser, M.; van der Hoorn, R.A.; Weerapana, E. Sulfonyl fluoride analogues as activity-based probes for serine proteases. Chembiochem 2012, 13, 2327–2330, doi:10.1002/cbic.201200531.
- Haedke, U.; Gotz, M.; Baer, P.; Verhelst, S.H. Alkyne derivatives of isocoumarins as clickable activity-based probes for serine proteases. Bioorg. Med. Chem. 2012, 20, 633–640, doi:10.1016/j.bmc.2011.03.014.
- Sabido, E.; Tarrago, T.; Giralt, E. Using peptidyl aldehydes in activity-based proteomics. Bioorg. Med. Chem. Lett. 2009, 19, 3752–3755, doi:10.1016/j.bmcl.2009.04.148.
- Sabido, E.; Tarrago, T.; Niessen, S.; Cravatt, B.F.; Giralt, E. Activity-based probes for monitoring postproline protease activity. ChemBioChem 2009, 10, 2361–2366, doi:10.1002/cbic.200900244.
- Wolf, E.V.; Zeissler, A.; Vosyka, O.; Zeiler, E.; Sieber, S.; Verhelst, S.H. A new class of rhomboid protease inhibitors discovered by activity-based fluorescence polarization. PLoS One 2013, 8, e72307.
- An, H.; Statsyuk, A.V. Development of activity-based probes for ubiquitin and ubiquitin-like protein signaling pathways. J. Am. Chem. Soc. 2013, 135, 16948–16962, doi:10.1021/ja4099643.
- Ciechanover, A. Intracellular protein degradation: From a vague idea through the lysosome and the ubiquitin-proteasome system and onto human diseases and drug targeting. Neurodegener. Dis. 2012, 10, 7–22, doi:10.1159/000334283.
- Powers, J.C.; Asgian, J.L.; Ekici, O.D.; James, K.E. Irreversible inhibitors of serine, cysteine, and threonine proteases. Chem. Rev. 2002, 102, 4639–4750, doi:10.1021/cr010182v.
- Greenbaum, D.; Medzihradszky, K.F.; Burlingame, A.; Bogyo, M. Epoxide electrophiles as activity-dependent cysteine protease profiling and discovery tools. Chem. Biol. 2000, 7, 569–581, doi:10.1016/S1074-5521(00)00014-4.
- Kaschani, F.; Verhelst, S.H.; van Swieten, P.F.; Verdoes, M.; Wong, C.S.; Wang, Z.; Kaiser, M.; Overkleeft, H.S.; Bogyo, M.; van der Hoorn, R.A. Minitags for small molecules: Detecting targets of reactive small molecules in living plant tissues using “click chemistry”. Plant J. 2009, 57, 373–385, doi:10.1111/j.1365-313X.2008.03683.x.
- Van der Linden, W.A.; Li, N.; Hoogendoorn, S.; Ruben, M.; Verdoes, M.; Guo, J.; Boons, G.J.; van der Marel, G.A.; Florea, B.I.; Overkleeft, H.S. Two-step bioorthogonal activity-based proteasome profiling using copper-free click reagents: A comparative study. Bioorg. Med. Chem. 2012, 20, 662–666, doi:10.1016/j.bmc.2011.06.037.
- Ovaa, H.; van Swieten, P.F.; Kessler, B.M.; Leeuwenburgh, M.A.; Fiebiger, E.; van den Nieuwendijk, A.M.; Galardy, P.J.; van der Marel, G.A.; Ploegh, H.L.; Overkleeft, H.S. Chemistry in living cells: Detection of active proteasomes by a two-step labeling strategy. Angew. Chem. Int. Ed. Engl. 2003, 42, 3626–3629, doi:10.1002/anie.200351314.
- Geurink, P.P.; van der Linden, W.A.; Mirabella, A.C.; Gallastegui, N.; de Bruin, G.; Blom, A.E.; Voges, M.J.; Mock, E.D.; Florea, B.I.; van der Marel, G.A.; et al. Incorporation of non-natural amino acids improves cell permeability and potency of specific inhibitors of proteasome trypsin-like sites. J. Med. Chem. 2013, 56, 1262–1275, doi:10.1021/jm3016987.
- Bicker, K.L.; Thompson, P.R. The protein arginine deiminases: Structure, function, inhibition, and disease. Biopolymers 2013, 99, 155–163, doi:10.1002/bip.22127.
- Luo, Y.; Knuckley, B.; Bhatia, M.; Pellechia, P.J.; Thompson, P.R. Activity-based protein profiling reagents for protein arginine deiminase 4 (PAD4): Synthesis and in vitro evaluation of a fluorescently labeled probe. J. Am. Chem. Soc. 2006, 128, 14468–14469, doi:10.1021/ja0656907.
- Slack, J.L.; Causey, C.P.; Luo, Y.; Thompson, P.R. Development and use of clickable activity based protein profiling agents for protein arginine deiminase 4. ACS Chem. Biol. 2011, 6, 466–476, doi:10.1021/cb1003515.
- Wang, Y.; Hu, S.; Fast, W. A click chemistry mediated in vivo activity probe for dimethylarginine dimethylaminohydrolase. J. Am. Chem. Soc. 2009, 131, 15096–15097, doi:10.1021/ja906432e.
- Denisov, I.G.; Makris, T.M.; Sligar, S.G.; Schlichting, I. Structure and chemistry of cytochrome P450. Chem. Rev. 2005, 105, 2253–2277, doi:10.1021/cr0307143.
- Wright, A.T.; Song, J.D.; Cravatt, B.F. A suite of activity-based probes for human cytochrome P450 enzymes. J. Am. Chem. Soc. 2009, 131, 10692–10700, doi:10.1021/ja9037609.
- Severi, E.; Hood, D.W.; Thomas, G.H. Sialic acid utilization by bacterial pathogens. Microbiology 2007, 153, 2817–2822, doi:10.1099/mic.0.2007/009480-0.
- Tsai, C.S.; Yen, H.Y.; Lin, M.I.; Tsai, T.I.; Wang, S.Y.; Huang, W.I.; Hsu, T.L.; Cheng, Y.S.; Fang, J.M.; Wong, C.H. Cell-permeable probe for identification and imaging of sialidases. Proc. Natl. Acad. Sci. USA 2013, 110, 2466–2471, doi:10.1073/pnas.1222183110.
- Overall, C.M.; Lopez-Otin, C. Strategies for MMP inhibition in cancer: Innovations for the post-trial era. Nat. Rev. Cancer 2002, 2, 657–672, doi:10.1038/nrc884.
- Sieber, S.A.; Niessen, S.; Hoover, H.S.; Cravatt, B.F. Proteomic profiling of metalloprotease activities with cocktails of active-site probes. Nat. Chem. Biol. 2006, 2, 274–281.
- You, A.; Tong, J.K.; Grozinger, C.M.; Schreiber, S.L. CoREST is an integral component of the CoREST- human histone deacetylase complex. Proc. Natl. Acad. Sci. USA 2001, 98, 1454–1458, doi:10.1073/pnas.98.4.1454.
- Salisbury, C.M.; Cravatt, B.F. Optimization of activity-based probes for proteomic profiling of histone deacetylase complexes. J. Am. Chem. Soc. 2008, 130, 2184–2194, doi:10.1021/ja074138u.
- Salisbury, C.M.; Cravatt, B.F. Activity-based probes for proteomic profiling of histone deacetylase complexes. Proc. Natl. Acad. Sci. USA 2007, 104, 1171–1176, doi:10.1073/pnas.0608659104.
- Kalesh, K.A.; Sim, D.S.; Wang, J.; Liu, K.; Lin, Q.; Yao, S.Q. Small molecule probes that target Abl kinase. Chem. Commun. 2010, 46, 1118–1120.
- Tantama, M.; Lin, W.C.; Licht, S. An activity-based protein profiling probe for the nicotinic acetylcholine receptor. J. Am. Chem. Soc. 2008, 130, 15766–15767, doi:10.1021/ja805868x.
- De Strooper, B.; Vassar, R.; Golde, T. The secretases: Enzymes with therapeutic potential in Alzheimer disease. Nat. Rev. Neurol. 2010, 6, 99–107, doi:10.1038/nrneurol.2009.218.
- Zettl, H.; Weggen, S.; Schneider, P.; Schneider, G. Exploring the chemical space of gamma-secretase modulators. Trends Pharmacol. Sci. 2010, 31, 402–410, doi:10.1016/j.tips.2010.05.007.
- Pozdnyakov, N.; Murrey, H.E.; Crump, C.J.; Pettersson, M.; Ballard, T.E.; Am Ende, C.W.; Ahn, K.; Li, Y.M.; Bales, K.R.; Johnson, D.S. gamma-Secretase modulator (GSM) photoaffinity probes reveal distinct allosteric binding sites on presenilin. J. Biol. Chem. 2013, 288, 9710–9720, doi:10.1074/jbc.M112.398602.
- Crump, C.J.; Johnson, D.S.; Li, Y.M. Development and mechanism of gamma-secretase modulators for Alzheimer’s disease. Biochemistry 2013, 52, 3197–3216, doi:10.1021/bi400377p.
- Crump, C.J.; am Ende, C.W.; Ballard, T.E.; Pozdnyakov, N.; Pettersson, M.; Chau, D.M.; Bales, K.R.; Li, Y.M.; Johnson, D.S. Development of clickable active site-directed photoaffinity probes for gamma-secretase. Bioorg. Med. Chem. Lett. 2012, 22, 2997–3000, doi:10.1016/j.bmcl.2012.02.027.
- Cravatt, B.F.; Simon, G.M.; Yates, J.R., 3rd. The biological impact of mass-spectrometry-based proteomics. Nature 2007, 450, 991–1000, doi:10.1038/nature06525.
- Weerapana, E.; Speers, A.E.; Cravatt, B.F. Tandem orthogonal proteolysis-activity-based protein profiling (TOP-ABPP)—A general method for mapping sites of probe modification in proteomes. Nat. Protoc. 2007, 2, 1414–1425, doi:10.1038/nprot.2007.194.
- Speers, A.E.; Cravatt, B.F. A tandem orthogonal proteolysis strategy for high-content chemical proteomics. J. Am. Chem. Soc. 2005, 127, 10018–10019, doi:10.1021/ja0532842.
- Weerapana, E.; Wang, C.; Simon, G.M.; Richter, F.; Khare, S.; Dillon, M.B.; Bachovchin, D.A.; Mowen, K.; Baker, D.; Cravatt, B.F. Quantitative reactivity profiling predicts functional cysteines in proteomes. Nature 2010, 468, 790–795, doi:10.1038/nature09472.
- Qian, Y.; Martell, J.; Pace, N.J.; Ballard, T.E.; Johnson, D.S.; Weerapana, E. An isotopically tagged azobenzene-based cleavable linker for quantitative proteomics. ChemBioChem 2013, 14, 1410–1414, doi:10.1002/cbic.201300396.
- Weerapana, E.; Simon, G.M.; Cravatt, B.F. Disparate proteome reactivity profiles of carbon electrophiles. Nat. Chem. Biol. 2008, 4, 405–407, doi:10.1038/nchembio.91.
- Wang, C.; Weerapana, E.; Blewett, M.M.; Cravatt, B.F. A chemoproteomic platform to quantitatively map targets of lipid-derived electrophiles. Nat. Methods 2014, 11, 79–85.
- Pace, N.J.; Weerapana, E. A competitive chemical-proteomic platform to identify zinc-binding cysteines. ACS Chem. Biol. 2013, doi:10.1021/cb400622q.
- Yang, J.; Chaurand, P.; Norris, J.L.; Porter, N.A.; Caprioli, R.M. Activity-based probes linked with laser-cleavable mass tags for signal amplification in imaging mass spectrometry: Analysis of serine hydrolase enzymes in mammalian tissue. Anal. Chem. 2012, 84, 3689–3695, doi:10.1021/ac300203v.
- Yan, X.W.; Luo, Y.C.; Zhang, Z.B.; Li, Z.X.; Luo, Q.; Yang, L.M.; Zhang, B.; Chen, H.F.; Bai, P.M.; Wang, Q.Q. Europium-labeled activity-based probe through click chemistry: Absolute serine protease quantification using 153Eu isotope dilution ICP/MS. Angew. Chem. Int. Ed. 2012, 51, 3358–3363, doi:10.1002/anie.201108277.
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