Next Article in Journal
Four New Furostanol Saponins from the Rhizomes and Roots of Smilax scobinicaulis and Their Cytotoxicity
Previous Article in Journal
Evaluation of the Efficiency of Different Disruption Methods on Yeast Cell Wall Preparation for β-Glucan Isolation
Article Menu

Export Article

Open AccessArticle
Molecules 2014, 19(12), 20962-20974; doi:10.3390/molecules191220962

Aortic Relaxant Activity of Crataegus gracilior Phipps and Identification of Some of Its Chemical Constituents

1
Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas, Querétaro 76010, Qro., Mexico
2
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Circuito exterior, Coyoacán 04510, Mexico D.F., Mexico
3
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Campus Juriquilla, Avenida de Las Ciencias S/N, Delegación Santa Rosa Jáuregui, Querétaro 76230, Qro., Mexico
*
Author to whom correspondence should be addressed.
Received: 29 October 2014 / Revised: 5 December 2014 / Accepted: 8 December 2014 / Published: 15 December 2014
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [744 KB, uploaded 15 December 2014]   |  

Abstract

This study focused on the assessment of the vasorelaxant activity of the organic and aqueous extracts obtained from leaves and fruits of a Mexican hawthorn (Crataegus gracilior) on isolated rat aorta, and on the purification and identification of some of their secondary metabolites by the use of chromatographic and spectroscopic techniques. The results obtained showed that the methanol extract has a significantly more potent and effective vasorelaxant effect than the other tested extracts, with an EC50 = 8.69 ± 4.34 µg/mL and an Emax = 94.6% ± 11.30%, values that are close to that of acetylcholine, the positive control. From the same extract, two major triterpenes were isolated and identified as ursolic and corosolic acids by comparison of their experimental NMR spectroscopic data with those reported in the literature. Chlorogenic acid, rutin, quercetin, kaempferol and (+)-catechin were also identified using HPLC coupled with PDAD. All these compounds have already been proven to possess on their own antihypertensive effect and other benefits on cardiovascular diseases and they can support, at least in part, the traditional use of this plant species. View Full-Text
Keywords: Crataegus gracilior; aorta; vasorelaxation; phenolics; triterpenes Crataegus gracilior; aorta; vasorelaxation; phenolics; triterpenes
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hernández-Pérez, A.; Bah, M.; Ibarra-Alvarado, C.; Rivero-Cruz, J.F.; Rojas-Molina, A.; Rojas-Molina, J.I.; Cabrera-Luna, J.A. Aortic Relaxant Activity of Crataegus gracilior Phipps and Identification of Some of Its Chemical Constituents. Molecules 2014, 19, 20962-20974.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top