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Molecules 2014, 19(11), 18543-18557; doi:10.3390/molecules191118543

In Vitro Proliferation and Production of Cytokine and IgG by Human PBMCs Stimulated with Polysaccharide Extract from Plants Endemic to Gabon

1
Institutde Pharmacopée et de MédecineTraditionnelles (IPHAMETRA), BP 1935 Libreville, Gabon
2
Centre International de Recherches Médicales de Franceville (CIRMF), BP 769 Franceville, Gabon
3
Laboratoire Glyco-MEV, IRIB, Université de Rouen, 76821 Mont-Saint-Aignan, France
Current address: Institut Jean-Pierre Bourgin UMR1318 INRA-AgroParisTech, INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), 78026 Versailles Cedex, France.
*
Author to whom correspondence should be addressed.
Received: 6 August 2014 / Revised: 7 October 2014 / Accepted: 8 October 2014 / Published: 13 November 2014
(This article belongs to the Collection Bioactive Compounds)
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Abstract

Polysaccharides were extracted from seven plants endemic to Gabon to study their potential immunological activities. Peripheral blood mononuclear cell (PBMC) (5 × 105 cells/mL) proliferation, cytokine and immunoglobulin G (IgG) assays were performed after stimulation with different concentrations of polysaccharide fractions compared with lipopolysaccharides (LPS) and concanavalin A (ConA) from healthy volunteers. The culture supernatants were used for cytokine and IgG detection by enzyme-linked immunosorbent assay (ELISA). The results show that pectin and hemicellulose extracts from Uvaria klainei, Petersianthus macrocarpus, Trichoscypha addonii, Aphanocalyx microphyllus, Librevillea klaineana, Neochevalierodendron stephanii and Scorodophloeus zenkeri induced production levels that were variable from one individual to another for IL-12 (3–40 pg/mL), IL-10 (6–443 pg/mL), IL-6 (7–370 pg/mL), GM-CSF (3–170 pg/mL) and IFN-γ (5–80 pg/mL). Only hemicelluloses from Aphanocalyx microphyllus produce a small amount of IgG (OD = 0.034), while the proliferation of cells stimulated with these polysaccharides increased up to 318% above the proliferation of unstimulated cells. However, this proliferation of PBMCs was abolished when the pectin of some of these plants was treated with endopolygalacturonase (p < 0.05), but the trend of cytokine synthesis remained the same, both before and after enzymatic treatment or saponification. This study suggests that these polysaccharides stimulate cells in a structure-dependent manner. The rhamnogalacturonan-I (RGI) fragment alone was not able to induce the proliferation of PBMC. View Full-Text
Keywords: pectins; hemicelluloses; cytokines; IgG; human PBMCs; endemic plants; Gabon pectins; hemicelluloses; cytokines; IgG; human PBMCs; endemic plants; Gabon
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Mengome, L.E.; Voxeur, A.; Akue, J.P.; Lerouge, P. In Vitro Proliferation and Production of Cytokine and IgG by Human PBMCs Stimulated with Polysaccharide Extract from Plants Endemic to Gabon. Molecules 2014, 19, 18543-18557.

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