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Molecules 2014, 19(1), 1258-1272; doi:10.3390/molecules19011258
Article

Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin

1, 2, 2, 3, 3, 2 and 2,*
Received: 11 December 2013; in revised form: 9 January 2014 / Accepted: 14 January 2014 / Published: 20 January 2014
(This article belongs to the Section Metabolites)
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Abstract: Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug.
Keywords: Polygonum cuspidatum; emodin; antiviral activity; EBV Polygonum cuspidatum; emodin; antiviral activity; EBV
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yiu, C.-Y.; Chen, S.-Y.; Yang, T.-H.; Chang, C.-J.; Yeh, D.-B.; Chen, Y.-J.; Lin, T.-P. Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin. Molecules 2014, 19, 1258-1272.

AMA Style

Yiu C-Y, Chen S-Y, Yang T-H, Chang C-J, Yeh D-B, Chen Y-J, Lin T-P. Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin. Molecules. 2014; 19(1):1258-1272.

Chicago/Turabian Style

Yiu, Ching-Yi; Chen, Shih-Ying; Yang, Tsai-Hsiu; Chang, Che-Jung; Yeh, Dong-Bor; Chen, Yi-Jie; Lin, Tsuey-Pin. 2014. "Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin." Molecules 19, no. 1: 1258-1272.


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