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Molecules 2014, 19(1), 1189-1200; doi:10.3390/molecules19011189
Article

Validation of a Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry Method for Determination of All-Trans Retinoic Acid in Human Plasma and Its Application to a Bioequivalence Study

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 and
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1 Institute of Clinical Pharmacology, Central South University, Changsha, Hunan 410078, China 2 Hunan Provincial Tumor Hospital, The Affiliated Tumor Hospital of Xiangya Medical School of Central South University, Changsha, Hunan 410013, China
* Author to whom correspondence should be addressed.
Received: 29 November 2013 / Revised: 25 December 2013 / Accepted: 7 January 2014 / Published: 17 January 2014
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Abstract

A sensitive, reliable and specific LC-MS-MS method was developed and validated for the identification and quantitation of all-trans retinoic acid (ATRA) in human plasma. Acitretin was used as the internal standard (IS). After liquid-liquid extraction of 500 μL plasma with methyl tert-butyl ether (MTBE), ATRA and the IS were chromatographed on a HyPURITY C18 column (150 mm × 2.1 mm, 5 μm) with the column temperature set at 40 °C. The mobile phase was consisted of 40% phase A (MTBE–methanol–acetic acid, 50:50:0.5, v/v) and 60% phase B (water–methanol–acetic acid, 50:50:0.5, v/v) with a flow rate of 0.3 mL/min. The API 4000 triple quadrupole mass spectrometer was operated in multiple reaction monitoring (MRM) mode via the positive electrospray ionization interface using the transition m/z 301.4 → 123.1 for ATRA and m/z 326.9 → 177.1 for IS, respectively. The calibration curve was linear over the range of 0.45–217.00 ng/mL (r ≥ 0.999) with a lower limit of quantitation (LLOQ) of 0.45 ng/mL. The intra- and inter-day precisions values were below 8% relative standard deviation and the accuracy was from 98.98% to 106.19% in terms of relative error. The validated method was successfully applied in a bioequivalence study of ATRA in Chinese healthy volunteers.
Keywords: all-trans retinoic acid (ATRA); LC-MS-MS; liquid-liquid extraction; bioequivalence all-trans retinoic acid (ATRA); LC-MS-MS; liquid-liquid extraction; bioequivalence
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Peng, J.-B.; Luo, C.-H.; Wang, Y.-C.; Huang, W.-H.; Chen, Y.; Zhou, H.-H.; Tan, Z.-R. Validation of a Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry Method for Determination of All-Trans Retinoic Acid in Human Plasma and Its Application to a Bioequivalence Study. Molecules 2014, 19, 1189-1200.

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