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Molecules 2013, 18(7), 8429-8439; doi:10.3390/molecules18078429
Article

Separation and Identification of 1,2,4-Trihydroxynaphthalene-1-O-glucoside in Impatiens glandulifera Royle

1,* , 1
, 2
 and 1
1 Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Centre, Academy of Sciences of the Czech Republic, Branišovská 31, České Budějovice 370 05, Czech Republic 2 Department of Organic Synthesis and Analytical Chemistry, Institute of Chemical Process Fundamentals, Academy of Sciences of the Czech Republic, Rozvojová 135, Praha 165 05, Czech Republic
* Author to whom correspondence should be addressed.
Received: 24 May 2013 / Revised: 10 July 2013 / Accepted: 10 July 2013 / Published: 17 July 2013
(This article belongs to the Section Metabolites)
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Abstract

Methanolic extract from lyophilized roots of Impatiens glandulifera Royle was analyzed by high performance liquid chromatography using DAD and FLD detection and this revealed one dominant highly fluorescent very unstable substance. The stability of this derivative is strongly dependent on the plant material drying procedure and extraction procedure used. The structure of the substance was established as 1,2,4-trihydroxynaphthalene-1-O-glucoside (THNG) according LC-MS and NMR measurements. When lyophilized plant material was extracted with methanol an almost four times higher amount of THNG was found in the extract, compared to the amount of 2-hydroxy-1,4-naphthoquinone obtained, while in the case of the same lyophilized plant material extracted with water there was no THNG in the extract. The main compounds in this case was 2-hydroxy-1,4-naphthoquinone. In the plant material dried at the laboratory temperature and extracted by methanol there are only traces of THNG.
Keywords: 1,2,4-trihydroxynaphthalene-1-O-glucoside; Impatients glandulifera Royle 1,2,4-trihydroxynaphthalene-1-O-glucoside; Impatients glandulifera Royle
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Tříska, J.; Vrchotová, N.; Sýkora, J.; Moos, M. Separation and Identification of 1,2,4-Trihydroxynaphthalene-1-O-glucoside in Impatiens glandulifera Royle. Molecules 2013, 18, 8429-8439.

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