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Molecules 2013, 18(7), 7549-7556; doi:10.3390/molecules18077549
Article

Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity

, , , *  and *
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
* Authors to whom correspondence should be addressed.
Received: 3 June 2013 / Revised: 25 June 2013 / Accepted: 26 June 2013 / Published: 27 June 2013
(This article belongs to the Section Natural Products)
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Abstract

Spatholobus suberectus Dunn, belonging to the legume family (Fabaceae), has been used as a Traditional Chinese Medicine for the treatment of anemia, menoxenia and rheumatism. A limited number of studies report that various types of flavonoids are the main characteristic constituents of this herb. We have now found that S. suberectus contains about 2% phenolic components and characterized the major phenolic components as homogeneous B-type procyanidin conjugates using a liquid chromatography with diode-array detection-ESI mass spectrometry (LC-DAD/ESI-MS) method. This is the first report on occurrence of most B-type procyanidins in this herb. Moreover, the total phenolics extract was assayed for inhibitory activity on human neutrophil elastase and its IC50 was found to be 1.33 μg/mL.
Keywords: Spatholobus suberectus; phenolic components; HPLC-DAD/ESI-MS; procyanidins; human neutrophil elastase Spatholobus suberectus; phenolic components; HPLC-DAD/ESI-MS; procyanidins; human neutrophil elastase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Huang, Y.; Chen, L.; Feng, L.; Guo, F.; Li, Y. Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity. Molecules 2013, 18, 7549-7556.

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