Molecules 2013, 18(7), 7549-7556; doi:10.3390/molecules18077549
Article

Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity

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Received: 3 June 2013; in revised form: 25 June 2013 / Accepted: 26 June 2013 / Published: 27 June 2013
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Spatholobus suberectus Dunn, belonging to the legume family (Fabaceae), has been used as a Traditional Chinese Medicine for the treatment of anemia, menoxenia and rheumatism. A limited number of studies report that various types of flavonoids are the main characteristic constituents of this herb. We have now found that S. suberectus contains about 2% phenolic components and characterized the major phenolic components as homogeneous B-type procyanidin conjugates using a liquid chromatography with diode-array detection-ESI mass spectrometry (LC-DAD/ESI-MS) method. This is the first report on occurrence of most B-type procyanidins in this herb. Moreover, the total phenolics extract was assayed for inhibitory activity on human neutrophil elastase and its IC50 was found to be 1.33 μg/mL.
Keywords: Spatholobus suberectus; phenolic components; HPLC-DAD/ESI-MS; procyanidins; human neutrophil elastase
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MDPI and ACS Style

Huang, Y.; Chen, L.; Feng, L.; Guo, F.; Li, Y. Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity. Molecules 2013, 18, 7549-7556.

AMA Style

Huang Y, Chen L, Feng L, Guo F, Li Y. Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity. Molecules. 2013; 18(7):7549-7556.

Chicago/Turabian Style

Huang, Youwu; Chen, Liang; Feng, Li; Guo, Fujiang; Li, Yiming. 2013. "Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity." Molecules 18, no. 7: 7549-7556.

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