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Article

Chemical Composition and Nematicidal Activity of Essential Oil of Agastache rugosa against Meloidogyne incognita

by
He Qin Li
1,
Qi Zhi Liu
1,
Zhi Long Liu
1,*,
Shu Shan Du
2,* and
Zhi Wei Deng
3
1
Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China
2
College of Resources Science and Technology, Beijing Normal University, Haidian District, Beijing 100875, China
3
Analytic and Testing Center, Beijing Normal University, Haidian District, Beijing 100875, China
*
Authors to whom correspondence should be addressed.
Molecules 2013, 18(4), 4170-4180; https://doi.org/10.3390/molecules18044170
Submission received: 26 February 2013 / Revised: 2 April 2013 / Accepted: 3 April 2013 / Published: 9 April 2013
(This article belongs to the Section Natural Products Chemistry)
Browse Figure
Versions Notes

Abstract

:
The aim of this research was to determine the chemical composition and nematicidal activity of essential oil of Agastache rugosa flowering aerial parts against the root knot nematode, Meloidogyne incognita, and to isolate and identify any nematicidal constituents from the essential oil. The essential oil of A. rugosa aerial parts was obtained by hydrodistillation and analyzed by GC-FID and GC-MS. A total of 37 components of the essential oil were identified, with the principal compounds being methyleugenol (50.51%), estragole (8.55%), and eugenol (7.54%), followed by thymol (3.62%), pulegone (2.56%), limonene (2.49%) and caryophyllene (2.38%). Based on bioactivity-guided fractionation, the three active constituents were isolated from the essential oil and identified as methyleugenol, estragole and eugenol. The essential oil of A. rugosa exhibited strong nematicidal activity against M. incognita, with a LC50 value of 47.3 μg/mL. The components eugenol (LC50 = 66.6 μg/mL) and methyleugenol (LC50 = 89.4 μg/mL) exhibited stronger nematicidal activity against M. incognita (LC50 = 185.9 μg/mL). The results indicate that the essential oil of A. rugosa aerial parts and its constituent compounds have potential for development into natural nematicides for control of the root knot nematode.

1. Introduction

Nematodes are tiny worms, some of them are parasites to plants, and can play an important role in the predisposition of the host plant to invasions by secondary pathogens. Meloidogyne incognita (Kofoid and White) Chitwood is the most economically important and widely distributed nematode throughout China and it is responsible for considerable crop losses [1]. Essential oils from different plant sources have demonstrated several biological activities, including antibacterial and antifungal [2,3], insecticidal [4,5,6], larvicidal [7,8,9], acaricidal [10], and nematicidal [1,11,12,13,14,15,16]. As a consequence, this vast arsenal of bioactive compounds has attracted significant and increasing attention of researchers in recent years [17,18,19,20]. During our mass screening program for new agrochemicals from local wild plants and Chinese medicinal herbs, the essential oil from Agastache rugosa (Fisch. et Mey.) Kuntze (family: Labiatae) flowering aerial parts has been found to possess nematicidal activity towards the root knot nematode, M. incognita.
A. rugosa has been used as a wild vegetable and herbal drug for the treatment of anorexia, vomiting and other intestinal disorders [21]. Chemical composition of the essential oils obtained from aerial parts (stem, flower, leaves) of A. rugosa grown in different countries has been the subject of some studies and a great variation in chemical composition of the essential oils were observed [22,23,24,25,26,27,28,29,30,31,32,33,34,35,36]. This plant has been proved to have antimicrobial [26,32], anti-fungal [30,31] and antiviral activity [28]. Methanol extract of A. rugosa whole plant possessed strong insecticidal activity against the cigarette beetle (Lasioderma serricorne) [37] and adults of the rice weevil (Sitophilus oryzae) and the adzuki bean weevil (Callosobruchus chinensis) [38]. However, a literature survey has shown that there are no reports on the nematidicidal activity of A. rugosa essential oil against root-knot nematodes, thus we decided to investigate for the first time the chemical constituents and nematicidal activity of the essential oil of A. rugosa against this species and to isolate any active constituent compounds from the essential oil.

2. Results and Discussion

2.1. Essential Oil Chemical Composition

The yield of A. rugosa essential oil was 0.32% (v/w) and the density of the essential oil was determined to be 0.91 g/mL. GC-MS analysis of the essential oil of A. rugosa aerial parts led to the identification and quantification of a total of 37 major components, accounting for 96.14% of the total components present (Table 1). The principal compounds in the essential oil of A. rugosa were methyleugenol (50.51%), estragole (8.55%), and eugenol (7.54%) (Figure 1), followed by thymol (3.62%), pulegone (2.56%), limonene (2.49%) and caryophyllene (2.38%). Most of the essential oil was phenylpropanoids (66.60%) and only 14.22% monoterpenoids and 13.34% sesquiterpenoids. The results are quite different from the previous reports. For example, estragole (ranging from 46.7 to 93.7%) was major compound of the essential oils of A. rugosa and A. foeniculum and putative hybrids collected from North America [22,39]. Moreover, the essential oils of A. rugosa collected from Europe [23,24], Vietnam [25], and Korea [28,29,30] all contained estragole as a principal compound.
Table
Table 1. Chemical constituents of the essential oil derived from Agastache rugosa aerial parts.
Table 1. Chemical constituents of the essential oil derived from Agastache rugosa aerial parts.
RI *CompoundComposition, %
Monoterpenoids 14.22
931α-Pinene0.52
984β-Pinene1.21
1029D-Limonene2.49
1057γ-Terpinene0.13
1188α-Terpineol0.34
1097Linalool1.77
1236Pulegone2.56
1288Cuminic alcohol1.38
1292Thymol3.62
Sesquiterpenoids 13.34
1313Elixene0.12
1317Carvacrol0.20
1350α-Cubebene0.13
1382iso-Ledene0.11
1385β-Bourbonene0.37
1393β-Elemen0.26
1420Caryophyllene2.38
1462cis-α-Farnesene0.17
1473γ-Muurolene0.41
1486Germacrene D1.45
1491Aromadendrene0.32
1499Bicyclogermacrene0.88
1500 α-Muurolene0.29
1511α-Farnesene0.72
1454α-Caryophyllene0.19
1521δ-Cadinene0.85
1546Cadina-4,9-diene0.28
1561Germacrene B0.68
1578Spatulenol1.11
1584Caryophyllene oxide0.73
1592Viridiflorol0.12
1642τ-Muurolol0.81
1652α-Cadinol0.96
Phenylpropanoids 66.60
1195Estragole8.55
1356Eugenol7.54
1369Methyleugenol50.51
Others 1.98
975Morrilol0.16
1066Acetophenone1.82
Total identified 96.14
* RI, retention index as determined on a HP-5MS column using the homologous series of n-hydrocarbons.
Molecules 18 04170 g001 550
Figure 1. Constituent compounds isolated from the essential oil of Agastache rugosa aerial parts.
Figure 1. Constituent compounds isolated from the essential oil of Agastache rugosa aerial parts.
Molecules 18 04170 g001
On the other hand, thymoquinone (70.11%) was the main component of the essential oil of A. rugosa aerial parts collected from northeast China [40], while estragole (74.84%) was the major compound of the essential oil of A. rugosa aerial parts collected from Hubei Province (Central China) and methyleugenol (49.89%) and estragole (19.45%) were two major compounds of the essential oil of A. rugosa aerial parts collected from Henan Province (Central China) [41]. Pulegone (37.58%) was the principal component of the essential oil of A. rugosa aerial parts collected from Zhejiang Province (Eastern China) [42]. Three chemoecological types of A. rugosa were consequently suggested as follows: type A with estragole as the main constituent, type B with methyleugenol as the principal constituent and type C with menthone derivates as the major constituents [43]. It seems that all the three chemotypic races of this species of plant are found in China. Our samples (from Beijing) belong to type B, with about 50% methyleugenol as the principal compound (Table 1), whereas samples collected from Central China belong to type A or type B [41]. Moreover, samples collected from Eastern China and Northeast China do not belong to any of the three types [40,42] because the major constituent compounds in those essential oils were thymoquinone and pulegone, respectively. Thus, further studies are needed to clarify variation of constituents of the Chinese A. rugosa oil. However, all these differences of chemical composition of the essential oils might have been due to harvest time and local, climatic and seasonal factors, as well as storage duration of the medicinal herbs. For practical use, it would be necessary to standardize the essential oil of Chinese A. rugosa.

2.2. Nematicidal Activity

The essential oil of A. rugosa aerial parts exhibited strong nematicidal activity against root knot nematode, M. incognita, with an LC50 value of 47.3 µg/mL (Table 2). Compared with the synthetic insecticide carbofuran, the essential oil of A. rugosa aerial parts possessed stronger toxicity against M. incognita, as carbofuran displayed a LC50 value of 72.3 μg/mL [1]. Among the three main components, both eugenol (LC50 = 66.6 μg/mL) and methyleugenol (LC50 = 89.4 μg/mL) exhibited stronger nematicidal activity against M. incognita than estragole (LC50 = 185.9 μg/mL). Compared with carbofuran, eugenol and methyleugenol exhibited a stronger or similar level of nematicidal activity towards M. incognita. In previous reports [44,45,46,47], eugenol and methyleugenol have been demonstrated to possess nematicidal activity against several nematode species, e.g., pine wood nematode (Bursaphelenchus xylophilus), Caenorhabditis elegans, and root knot nematode, M. incognita. However, all the three isolated constituent compounds possessed less activity against the root knot nematode than the crude essential oil (Table 2), suggesting that there may be stronger active compound(s) in small amounts in the essential oil or maybe some synergistic action between the various compounds. For example, carvacrol and thymol immobilized the juveniles of the root knot nematode M. javanica and inhibited hatching at >125 μL/L in vitro and the two components mixed in sandy soil at concentrations of 75 and 150 mg/kg reduced root galling of cucumber seedlings [17]. Carvacrol and thymol exhibited nematicidal activity against root knot nematode, M. incognita, with 76 h LC50 values of 176 μg/mL and 280 μg/mL, respectively [45]. In previous reports, a synergistic effect among terpene constituents of the essential oils from seven plants indigenous to Greece has been detected [18,46]. The most potent terpene pairs between which synergistic actions were found, in decreasing order, were: trans-anethole/geraniol, trans-anethole/eugenol, carvacrol/eugenol and geraniol/carvacrol.
Table
Table 2. Nematicidal activity of the essential oil of Agastache rugosa aerial parts and its three main components against Meloidogyne incognita.
Table 2. Nematicidal activity of the essential oil of Agastache rugosa aerial parts and its three main components against Meloidogyne incognita.
TreatmentsConcentrations (μg/mL)LC50 (μg/mL) 95% FL *LC90 (μg/mL) 95% FL *Slope ± SEChi square (χ2 )
A. rugosa12.5-200.047.3 (42.9–55.2)174.6 (156.9–191.1)6.51 ± 0.669.06
Estragole80.0-860.0185.9 (169.7–206.1)463.6 (422.9–489.5)7.03 ± 0.687.64
Eugenol12.5-200.066.6 (60.6–74.1)182.3 (164.8–198.6)5.21 ± 0.539.77
Methyleugenol40.0-240.089.4 (79.7–98.1)193.7 (176.7–214.9)8.36 ± 0.788.25
Carbofuran **25.0-400.072.3 (37.9–118.0)--13.57
* Fiducial limits; ** From Bai et al. [11].
Andres et al. [18] also demonstrated that there was a synergistic interaction between some of the constituent compounds (carvone, 1,8-cineole and menthol) at certain concentrations. Considering the positive control is a synthetic insecticide, the observed nematicidal activity of the essential oil of A. rugosa and the three constituent compounds, especially eugenol and methyleugenol, is quite promising and the essential oil and its individual constituents show potential to be developed as possible natural nematicides for the control of the root knot nematodes, although for the practical application of the essential oil and the isolated constituent compounds as novel nematicides, further studies on the safety of the essential oil/pure compounds to humans and on development of formulations are necessary to improve the efficacy and stability and to reduce cost.

3. Experimental

3.1. Plant Material and Essential Oil Extraction

Fresh aerial parts (10 kg of leaves, stems and flowers) of A. rugosa were harvested in August 2009 from Xiaolongmeng National Forest Park (Mentougou District, Beijing 102300, 38.24° N latitude and 115.20° E longitude). The species was identified by Dr. Liu, QR (College of Life Sciences, Beijing Normal University, China), and the voucher specimen (CMH-TuHuoXiang-Beijing-2009-08) was deposited in the museum of Department of Entomology, China Agricultural University. The aerial parts were air-dried for one week and ground to a powder using a grinding mill (Retsch Muhle, Germany). The powder was subjected to hydrodistillation using a modified Clevenger-type apparatus for 6 h and extracted with n-hexane. Anhydrous sodium sulphate was used to remove water after extraction. The essential oil was stored in airtight containers in a refrigerator at 4 °C for subsequent experiments.

3.2. Gas Chromatography-Mass Spectrometry

Components of the essential oil of A. rugosa aerial parts were separated and identified by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS) using an Agilent 6890N gas chromatography system connected to an Agilent 5973N mass selective detector. The same column and analysis conditions were used for both GC-FID and GC-MS. They were equipped with capillary column with HP-5MS (30 m × 0.25 mm × 0.25 μm). The GC settings were as follows: the initial oven temperature was held at 60 °C for 1 min and ramped at 10 °C min−1 to 180 °C where it was held for 1 min, and then ramped at 20 °C min–1 to 280 °C and held there for 15 min. The injector temperature was maintained at 270 °C. The samples (1 μL, after diluted to 1% with acetone) were injected, with a split ratio of 1: 10. The carrier gas was helium at flow rate of 1.0 mL min–1. Spectra were scanned from 20 to 550 m/z at 2 scans s–1. Most constituents were identified by gas chromatography by comparison of their retention indices with those of the literature or with those of authentic compounds available in our laboratories. The retention indices were determined in relation to a homologous series of n-alkanes (C8–C24) under the same operating conditions. Further identification was made by comparison of their mass spectra with those stored in NIST 05 and Wiley 275 libraries or with mass spectra from the literature [48]. Relative percentages of the individual components of the essential oil were obtained by averaging the GC-FID peak area% reports.

3.3. Purification and Characterization of Three Constituent Compounds

The crude essential oil of A. rugosa aerial parts (25 mL) was chromatographed on a silica gel (Merck 9385, 1,000 g) column (85 mm i.d., 850 mm length) by gradient elution with a mixture of solvents (n-hexane, n-hexane-ethyl acetate). Fractions of 500 mL were collected and concentrated at 40 °C, and similar fractions according to their TLC profiles were combined to yield 15 fractions. Fractions 5–8, 10, 12 that possessed nematicidal toxicity, and with similar TLC profiles, were pooled and further purified by preparative silica gel column chromatography (PTLC) with petroleum ether-acetone (50:1, v/v) until to obtain the pure compounds for determining their structures based on nuclear magnetic resonance spectroscopy. 1H and 13C-NMR spectra were recorded on a Bruker AMX500 [500 MHz (1H)] instrument using CDCl3 as the solvent with TMS as internal standard. Electron impact mass spectra (EIMS) were determined on a Micromass VG7035 mass spectrometer at 70 eV (probe).

3.4. Isolated Constituent Compounds

Estragole (1, Figure 1). A colorless oil (0.3 g), C10H12O. 1H-NMR (CDCl3) δ (ppm): 7.27 (1H, d, H-3, 5), 7.01 (1H, d, H-2, 6), 6.14 (1H, m, H-8), 5.26 (2H, d, H-9), 3.92 (3H, s, 10-CH3), 3.50 (2H, d, H-7). 13C-NMR (125 MHz, CDCl3) δ (ppm): 158.22 (C-8), 138.08 (C-1), 132.16 (C-9), 129.61 (C-4), 115.52 (C-3, C-5), 113.99 (C-2, C-6), 55.18(C-10), 39.52 (C-7). EI-MS m/z (%): 149 (13), 148 (100), 147 (46), 133 (23), 121 (41), 117 (32), 105 (23), 91 (20), 77 (23). The spectral data matched that given in a previous report [6].
Eugenol (2, Figure 1). A colorless oil (0.4 g), C10H12O2. 1H-NMR (CDCl3) δ (ppm): δ: 6.83 (1H, d, J = 8.8 Hz, H-5), 6.66 (1H, dd, J = 8.4, 1.8 Hz, H-6), 6.65 (1H, d, J = 1.8 Hz, H-2), 5.94 (1H, m, H-8), 5.73 (1H, br.s, D2O exchangeable, -OH), 5.06 (2H, m, H-9), 3.81 (3H, s, -OCH3), 3.30 (2H, dt, J = 6.6, 1.5 Hz, H-7). 13C-NMR (125 MHz, CDCl3) δ (ppm): 146.60 (C-3), 144.03 (C-4), 137.91 (C-8), 131.94 (C-1), 115.49 (C-5), 114.46 (C-2), 111.26 (C-9), 55.84 (C-10), 39.92 (C-7). EI-MS m/z (%): 165 (11), 164 (100), 149 (29), 137 (15), 133 (15), 121 (14), 103 (19), 91 (14), 77 (21), 55 (18). The spectral data matched that given in a previous report [49].
Methyleugenol (3, Figure 1). A colorless oil (0.8 g), C11H14O2. 1H-NMR (CDCl3) δ (ppm): 6.81 (1H, d, J = 8.0, H-6), 6.74 (1H, dd, J = 8.0, 2.0, H-5), 6.71 (1H, d, J = 2.0 H-2), 5.96 (1H, ddt, J = 17.0, 10.4, 6.8, H-8), 5.07 (2H, m, H-9), 3.86 (3H, s, -OCH3), 3.87 (3H, s, -OCH3), 3.34 (2H, ddd, J = 6.8, 1.7, 1.4, H-7). 13C-NMR (125 MHz, CDCl3) δ (ppm): 148.8 (C-4), 147.3 (C-3), 137.7 (C-8), 132.6 (C-6), 120.4 (C-1), 115.6 (C-9), 111.8 (C-2), 111.2 (C-5), 55.9(-OCH3), 55.7 (-OCH3), 39.8 (C-7). EI-MS m/z (%): 178 (100), 163 (33), 151 (15), 147 (32), 135 (18), 131 (11), 107 (28), 103 (39), 91 (50), 77 (26). The spectra data matched with previous report [50].

3.5. Nematicidal Assay

Egg masses of M. incognita obtained from tomato roots with aid of a stereomicroscope were maintained in Petri dishes during 24 h in distilled H2O for the juveniles to hatch. Range-finding studies were run to determine the appropriate testing concentrations. A serial dilution of A. rugosa essential oil (five concentrations) and pure compounds (five concentrations) was prepared in H2O solution with 2% DMSO. Aliquots of H2O (20 μL) containing ca. 100 juveniles (J2) were transferred to vials to which 980 μL of the solution containing essential oil or pure compounds was added. The vials were kept in a hood at 25 °C. The counting of the inactive nematodes was performed at every 24 h for 72 h. After the last count, the inactive juveniles were maintained in distilled H2O for 24 h to observe their revival. Six repetitions for each treatment were performed using H2O and a 2% DMSO in H2O solution as control. The experiments were repeated in three times. Results from all replicates for the pure compounds and essential oil were subjected to probit analysis using the PriProbit Program V1.6.3 to determine LC50 (median lethal concentration) values and their 95% confidence intervals (CI 95%) [51].

4. Conclusions

The study indicates that the essential oil of A. rugosa aerial parts and its main constituent compounds, eugenol, methyleugenol and estragole, have potential for development into natural nematicides for the control of root knot nematodes.

Acknowledgments

This project was supported by the Hi-Tech Research and Development of China 2011AA10A202. We thank Liu Q.R. from the College of Life Sciences, Beijing Normal University, Beijing 100875, for the identification of the investigated plants.

References

  1. Bai, C.Q.; Liu, Z.L.; Liu, Q.Z. Nematicidal constituents from the essential oil of Chenopodium ambrosioides aerial parts. E-J. Chem. 2011, 8, 143–148. [Google Scholar] [CrossRef]
  2. Wang, J.H.; Zhao, J.L.; Liu, H.; Zhou, L.; Liu, Z.L.; Han, J.G.; Zhu, Y.; Yang, F.Y. Chemical analysis and biological activity of the essential oils of two Valerianaceous species from China: Nardostachys chinensis and Valeriana. officinalis. Molecules 2010, 15, 6411–6422. [Google Scholar] [CrossRef]
  3. Wang, J.H.; Xu, L.; Yang, L.; Liu, Z.L.; Zhou, L. Composition, antibacterial and antioxidant activities of essential oils from Ligusticum sinense and L. jeholense (Umbelliferae) from China. Rec. Nat. Prod. 2011, 5, 314–318. [Google Scholar]
  4. Chu, S.S.; Hu, J.F.; Liu, Z.L. Composition of essential oil of Chinese Chenopodium ambrosioides and insecticidal activities to maize weevil, Sitophilus zeamais. Pest. Manag. Sci. 2011, 67, 714–718. [Google Scholar] [CrossRef]
  5. Chu, S.S.; Jiang, G.H.; Liu, Z.L. Insecticidal compounds from the essential oil of Chinese medicinal herb, Atractylodes chinensis. Pest. Manag. Sci. 2011, 67, 1253–1257. [Google Scholar] [CrossRef]
  6. Zhao, N.N.; Zhou, L.; Liu, Z.L.; Du, S.S.; Deng, Z.W. Evaluation of toxicities of some common spices essential oils from China against Liposcelis bostrychophila. Food Control. 2012, 26, 486–490. [Google Scholar] [CrossRef]
  7. Liu, Z.L.; He, Q.; Chu, S.S.; Wang, C.F.; Du, S.S.; Deng, Z.W. Essential oil composition and larvicidal activity of Saussurea lappa roots against the mosquito Aedes albopictus (Diptera: Culicidae). Parasitol. Res. 2012, 110, 2125–2130. [Google Scholar] [CrossRef]
  8. Liu, Z.L.; Liu, Q.Z.; Du, S.S.; Deng, Z.W. Mosquito larvicidal activity of alkaloids and limonoids derived from Evodia rutaecarpa unripe fruits against Aedes albopictus (Diptera: Culicidae). Parasitol. Res. 2012, 111, 991–996. [Google Scholar] [CrossRef]
  9. Liu, X.C.; Dong, H.W.; Zhou, L.; Du, S.S.; Liu, Z.L. Essential oil composition and larvicidal activity of Toddalia asiatica roots against the mosquito Aedes albopictus (Diptera: Culicidae). Parasitol. Res. 2013. [Google Scholar] [CrossRef]
  10. Sung, B.K.; Lee, H.S. Chemical composition and acaricidal activities of constituents derived from Eugenia caryophyllata leaf oils. Food Sci. Biotechnol. 2005, 14, P73–P76. [Google Scholar]
  11. Li, H.Q.; Bai, C.Q.; Chu, S.S.; Zhou, L.; Du, S.S.; Liu, Z.L.; Liu, Q.Z. Chemical composition and toxicities of the essential oil derived from Kadsura heteroclita stems against Sitophilus zeamais and Meloidogyne incognita. J. Med. Plants Res. 2011, 5, 4943–4948. [Google Scholar]
  12. Liu, Q.Z.; Li, H.Q.; Liu, Z.L. Nematicidal constituents from the ethanol extract of Evodia rutaecarpa Hort unripe fruits. J. Chem. 2013. [Google Scholar] [CrossRef]
  13. Lahlou, M. Methods to study the phytochemistry and bioactivity of essential oils. Phytother. Res. 2004, 18, 435–448. [Google Scholar] [CrossRef]
  14. Ntalli, N.G.; Manconi, F.; Leonti, M.; Maxia, A.; Caboni, P. Aliphatic ketones from Ruta chalepensis (Rutaceae) induce paralysis on root knot nematodes. J. Agric. Food Chem. 2011, 59, 7098–7103. [Google Scholar] [CrossRef]
  15. Oka, Y.; Nacar, S.; Putievsky, E.; Ravid, U.; Yaniv, Z.; Spiegel, Y. Nematicidal activity of essential oils and their components against the root-knot nematode. Phytopathology 2000, 90, 710–715. [Google Scholar] [CrossRef]
  16. Al-Banna, L.; Darwish, R.M.; Aburjai, T. Effect of plant extracts and EOs on root-knot nematode. Phytopathol. Mediterr. 2003, 42, 123–128. [Google Scholar]
  17. Ntalli, N.G.; Caboni, P. Botanical nematicides: A review. J. Agric. Food Chem. 2012, 60, 9929–9940. [Google Scholar] [CrossRef]
  18. Andres, M.F.; Gonzalez-Coloma, A.; Sanz, J.; Burillo, J.; Sainz, P. Nematicidal activity of essential oils: A review. Phytochem. Rev. 2013. [Google Scholar] [CrossRef]
  19. Isman, M.B. Plant essential oils for pest and disease management. Crop. Prot. 2000, 19, 603–608. [Google Scholar] [CrossRef]
  20. Isman, M.B. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Ann. Rev. Entomol. 2006, 51, 45–66. [Google Scholar] [CrossRef]
  21. Jinagsu New Medical College, Dictionary of Chinese Herbal Medicine; Shanghai Science & Technology Press: Shanghai, China, 1977; pp. 2710–2712.
  22. Charles, D.J.; Simon, J.E; Widrlechner, M.P. Characterization of the essential oil of Agastache species. J. Agric. Food Chem. 1991, 39, 1946–1949. [Google Scholar] [CrossRef]
  23. Weyerstahl, P.; Marschall, H.; Manteuffel, E. Volatile constituents of Agastache rugosa. J. Essent. Oil Res. 1992, 4, 585–587. [Google Scholar]
  24. Svoboda, K.P.; Gough, J.; Hampson, J.; Galambosi, B. Analysis of the essential oils of some Agastache. species grown in Scotland from various seed sources. Flav. Fragr. J. 1995, 10, 139–145. [Google Scholar] [CrossRef]
  25. Nguyen, X.D.; Luu, D.C.; Nguyen, H.T.; La, D.M.; Le, V.H.; Leclercq, P.A. Constituents of the leaf and flower oils of Agastache rugosa (Fisch. et Mey) O. Kuntze from Vietnam. J. Essent. Oil Res. 1996, 8, 135–138. [Google Scholar] [CrossRef]
  26. Song, J.H.; Kim, M.J.; Kwon, H.D.; Lee, W.K.; Park, I.H. Antimicrobial activity and characterization of volatile flavor extracts from Agastache rugosa. J. Food Sci. Nutr. 1999, 4, 97–102. [Google Scholar]
  27. Yang, D.; Wang, F.; Su, J.; Zeng, L. Chemical composition of essential oil in stems, leaves and flowers of Agastache rugosa. J. Chin. Med. Mater. 2000, 23, 149–151. [Google Scholar]
  28. Kim, T.H.; Shin, J.H.; Baek, H.H.; Lee, H.J. Volatile flavour compounds in suspension culture of Agastache rugosa Kuntze (Korean mint). J. Sci. Food Agric. 2001, 81, 569–575. [Google Scholar] [CrossRef]
  29. Shin, S.; Kim, Y.S.; Kang, C.A. Production of volatile oil components by cell culture of Agastache rugosa O. Kuntze. Nat. Prod. Sci. 2001, 7, 120–123. [Google Scholar]
  30. Shin, S.; Kang, C.A. Antifungal activity of the essential oil of Agastache rugosa Kuntze and its synergism with ketoconazole. Lett. Appl. Microbiol. 2003, 36, 111–115. [Google Scholar] [CrossRef]
  31. Shin, S. Essential oil compounds from Agastache rugosa as antifungal agents against Trichophyton species. Arch. Pharm. Res. 2004, 27, 295–299. [Google Scholar] [CrossRef]
  32. Chae, Y.A.; Hyun-Choong, O.; Song, J.S. Variability of the volatile composition of Agastache rugosa in South Korea. Acta. Hort. 2005, 675, 59–64. [Google Scholar]
  33. Kim, J. Phytotoxic and antimicrobial activities and chemical analysis of leaf essential oil from Agastache rugosa. J. Plant. Biol. 2008, 51, 276–283. [Google Scholar] [CrossRef]
  34. Skakovskii, E.D.; Kiselev, W.P.; Tychinskaya, L.Y; Schutova, A.G.; Gonsharova, L.W.; Spiridowish, E.W.; Bovdey, N.A.; Kiselev, P.A.; Gaidukevich, O.A. Characterization of the essential oil of Agastache rugosa by NMR spectroscopy. J. Appl. Spectrosc. 2010, 77, 329–334. [Google Scholar] [CrossRef]
  35. Jun, H.J.; Chung, M.J.; Dawson, K.; Rodriguez, R.L.; Houng, S.J.; Cho, S.Y.; Jeun, J.; Kim, J.Y.; Kim, K.H.; Park, K.W. Nutrigenomic analysis of hypolipidemic effects of Agastache rugosa essential oils in HepG2 cells and C57BL/6 mice. Food Sci. Biotechnol. 2010, 19, 219–227. [Google Scholar] [CrossRef]
  36. Mo, J.X.; Ma, L. Volatile oil of Herba agastache in various growth periods and different parts by GC-MS. Chin. J. Pharm. 2011, 42, 268–270. [Google Scholar]
  37. Kim, S.I.; Park, C.; Ohh, M.H.; Cho, H.C.; Ahn, Y.J. Contact and fumigant activities of aromatic plant extracts and essential oils against Lasioderma serricorne (Coleoptera: Anobiidae). J. Stored Prod. Res. 2003, 39, 11–19. [Google Scholar] [CrossRef]
  38. Kim, S.I.; Roh, J.Y.; Kim, D.H.; Lee, H.S.; Ahn, Y.J. Insecticidal activities of aromatic plant extracts and essential oils against Sitophilus oryzae and Callosobruchus chinensis. J. Stored Prod. Res. 2003, 39, 293–303. [Google Scholar] [CrossRef]
  39. Wilson, L.A.; Senechal, N.P.; Widrlechnerr, M.P. Headspace analysis of the volatile oils of Agsstache. J. Agric. Food Chem. 1992, 40, 1362–1366. [Google Scholar] [CrossRef]
  40. Yue, J.L.; Pan, X.F.; Wang, J.C. Chemical constituents of essential oil of Agastache rugosa of northeast China. J. Northeast Forestry Univ. 1998, 26, 72–74. [Google Scholar]
  41. Wang, D.M.; Yang, D.B.; Wang, F.S.; Wu, H.Y.; Gu, S.Y. Chemical constituent of Agastache. rugosa essential oils and physical forms of Agastache rugosa. Chin. Tradit. Herb. Drugs 2005, 36, 1302–1303. [Google Scholar]
  42. Mo, J.X.; Jiang, C.; Zhang, X.Y. Studies on characteristics of volatile oil and micro-identification between Herba Pogostamonis and Herba Agastachis Rugosae. J. Chin. Med. Mater. 2009, 32, 1675–1677. [Google Scholar]
  43. Fujita, S.; Fujita, Y. Miscellaneous contributions to essential oils of the plants from various territories. XXXIII. Essential oil of Agastache rugosa. Yakugaku Zasshi 1973, 93, 1679–1681. [Google Scholar]
  44. Park, I.K.; Kim, J.; Lee, S.G.; Shin, S.C. Nematicidal activity of plant essential oils and components from ajowan (Trachyspermum ammi), allspice (Pimenta dioica) and litsea (Litsea cubeba) essential oils against pine wood nematode (Bursaphelenchus xylophilus). J. Nematol. 2007, 39, 275–279. [Google Scholar]
  45. Ntalli, N.G.; Ferrari, F.; Giannakou, I.; Menkissoglu-Spiroudi, U. Phytochemistry and nematicidal activity of the essential oils from 8 Greek Lamiaceae aromatic plants and 13 terpene components. J. Agric. Food Chem. 2010, 58, 7856–7863. [Google Scholar] [CrossRef]
  46. Ntalli, N.G.; Ferrari, F.; Giannakou, I.; Menkissoglu-Spiroudi, U. Synergistic and antagonistic interactions of terpenes against Meloidogyne incognita and the nematicidal activity of essential oils from seven plants indigenous to Greece. Pest. Manag. Sci. 2011, 67, 341–351. [Google Scholar] [CrossRef]
  47. Abdel-Rahman, F.H.; Alaniz, N.M.; Saleh, M.A. Nematicidal activity of terpenoids. J. Environ. Sci. Health 2013, 48B, 16–22. [Google Scholar]
  48. Adams, R.P. Identification of Essential Oil Components by Gas Chromatography/Quadrupole. Mass Spectroscopy; Allured: Carol Stream, IL, USA, 2007. [Google Scholar]
  49. Mohottalage, S.; Tabacchi, R.; Guerin, P.M. Components from Sri Lankan Piper betel L. leaf oil and their analogues showing toxicity against the housefly, Musca domestica. Flav. Fragr. J. 2007, 22, 130–138. [Google Scholar] [CrossRef]
  50. Miyazawa, M.; Kohno, G. Suppression of chemical mutagen-induced SOS response by allylbenzen from Asiasarum heterotropoides in the Salmonella typhimurium TA1535/pSK1002 umu test. Nat. Prod. Res. 2005, 19, 29–36. [Google Scholar] [CrossRef]
  51. Sakuma, M. Probit analysis of preference data. Appl. Entomol. Zool. 1998, 33, 339–347. [Google Scholar]
  • Sample Availability: Samples of the crude extracts and pure compounds are available from the authors.

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MDPI and ACS Style

Li, H.Q.; Liu, Q.Z.; Liu, Z.L.; Du, S.S.; Deng, Z.W. Chemical Composition and Nematicidal Activity of Essential Oil of Agastache rugosa against Meloidogyne incognita. Molecules 2013, 18, 4170-4180. https://doi.org/10.3390/molecules18044170

AMA Style

Li HQ, Liu QZ, Liu ZL, Du SS, Deng ZW. Chemical Composition and Nematicidal Activity of Essential Oil of Agastache rugosa against Meloidogyne incognita. Molecules. 2013; 18(4):4170-4180. https://doi.org/10.3390/molecules18044170

Chicago/Turabian Style

Li, He Qin, Qi Zhi Liu, Zhi Long Liu, Shu Shan Du, and Zhi Wei Deng. 2013. "Chemical Composition and Nematicidal Activity of Essential Oil of Agastache rugosa against Meloidogyne incognita" Molecules 18, no. 4: 4170-4180. https://doi.org/10.3390/molecules18044170

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