Molecules 2013, 18(2), 2255-2265; doi:10.3390/molecules18022255
Abstract

Characterization of Proanthocyanidins in Stems of Polygonum multiflorum Thunb as Strong Starch Hydrolase Inhibitors

1, 1, 2,3, 3, 3, 3, 2, 2 and 1,* email
Received: 10 December 2012; in revised form: 5 February 2013 / Accepted: 5 February 2013 / Published: 18 February 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Characterzation of polyphenolic compounds in the stems of P. multiflorum was conducted using HPLC, high resolution LC-MS and LC-MSn. Proanthocyanidins in particular were isolated in 4.8% yield using solvent extraction followed by Sephadex LH-20 fractionation. HPLC analysis using a diol column revealed oligomers (from dimer to nonamer) as minor components, with (epi)catechin monomeric units predominating, and oligomers with higher degree of polymerization being dominant. Thiolysis treatment of the proanthocyanidins using mercaptoacetic acid produced thioether derivatives of (epi)catechin as the major product and a mean value of the degree of polymerization of 32.6 was estimated from the ratio of terminal and extension units of the (epi)catechin. The isolated proanthocyanidins were shown to strongly inhibit α-amylase with an acarbose equivalence (AE) value of 1,954.7 µmol AE/g and inhibit α-glucosidase with an AE value of 211.1 µmol AE/g.
Keywords: P. multiflorum; proanthocyanidins; α-amylase; (epi)catechin
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MDPI and ACS Style

Wang, H.; Song, L.; Feng, S.; Liu, Y.; Zuo, G.; Lai, F.; He, G.; Chen, M.; Huang, D. Characterization of Proanthocyanidins in Stems of Polygonum multiflorum Thunb as Strong Starch Hydrolase Inhibitors. Molecules 2013, 18, 2255-2265.

AMA Style

Wang H, Song L, Feng S, Liu Y, Zuo G, Lai F, He G, Chen M, Huang D. Characterization of Proanthocyanidins in Stems of Polygonum multiflorum Thunb as Strong Starch Hydrolase Inhibitors. Molecules. 2013; 18(2):2255-2265.

Chicago/Turabian Style

Wang, Hongyu; Song, Lixia; Feng, Shengbao; Liu, Yuancai; Zuo, Gang; Lai, Fuli; He, Guangyuan; Chen, Mingjie; Huang, Dejian. 2013. "Characterization of Proanthocyanidins in Stems of Polygonum multiflorum Thunb as Strong Starch Hydrolase Inhibitors." Molecules 18, no. 2: 2255-2265.

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