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Molecules 2013, 18(12), 15648-15661; doi:10.3390/molecules181215648

Preparative Isolation and Purification of Five Flavonoid Glycosides and One Benzophenone Galloyl Glycoside from Psidium guajava by High-Speed Counter-Current Chromatography (HSCCC)

1
Department of Traditional Chinese Medicine Chemistry, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, No. 6 Wangjing Zhonghuan South Road, Chaoyang District, Beijing 100102, China
2
Health-cultivation Laboratory of the Ministry Education, Beijing University of Chinese Medicine, 11 North Third Ring Road East, Chaoyang District, Beijing 100029, China
3
Chemistry of Chinese Medicine, Jilin Academy of Chinese Medicine Sciences, Changchun 130021, China
*
Author to whom correspondence should be addressed.
Received: 17 October 2013 / Revised: 9 December 2013 / Accepted: 10 December 2013 / Published: 16 December 2013
(This article belongs to the Section Natural Products)
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Abstract

Psidium guajava leaves have a diverse phytochemical composition including flavonoids, phenolics, meroterpenoids and triterpenes, responsible for the biological activities of the medicinal parts. In particular, flavonol glycosides show beneficial effects on type II diabetes mellitus. A simple and efficient HSCCC method has been developed for the preparative separation of five flavonoid glycosides and one diphenylmethane glycoside from P. guajava. A solvent system composed of n-hexane–ethyl acetate–methanol–water (0.7:4:0.8:4, v/v/v/v) was optimized for the separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. Under the optimized conditions, hyperoside (15.3 mg), isoquercitrin (21.1 mg), reynoutrin (65.2 mg), quercetin-3-O-β-D-arabinopyranoside (71.7 mg), quercetin-3-O-α-L-arabinofuranoside (105.6 mg) and 2,4,6-trihydroxy-3,5-dimethylbenzophenone 4-O-(6''-O-galloyl)-β-D-glucopyranoside (98.4 mg) were separated from crude sample (19.8 g). The structures of all the isolates were identified by ESI-MS, 1H- and 13C-NMR analyses and their purities (>95%) were determined using HPLC.
Keywords: HSCCC; flavonoid glycosides; benzophenone galloyl glycoside; Psidium guajava HSCCC; flavonoid glycosides; benzophenone galloyl glycoside; Psidium guajava
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MDPI and ACS Style

Zhu, Y.; Liu, Y.; Zhan, Y.; Liu, L.; Xu, Y.; Xu, T.; Liu, T. Preparative Isolation and Purification of Five Flavonoid Glycosides and One Benzophenone Galloyl Glycoside from Psidium guajava by High-Speed Counter-Current Chromatography (HSCCC). Molecules 2013, 18, 15648-15661.

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