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Anti-Tumor Effects of Atractylenolide I Isolated from Atractylodes macrocephala in Human Lung Carcinoma Cell Lines
Department of Oncology, Chengdu Military General Hospital, Chengdu 610083, Si Chuan Province, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 2 September 2013; in revised form: 1 October 2013 / Accepted: 16 October 2013 / Published: 29 October 2013
Abstract: Atractylenolide I (ATL-1) is the major sesquiterpenoid of Atractylodes macrocephala. This study was designed to investigate whether ATL-1 induced apoptosis in A549 and HCC827 cells in vitro and in vivo. In our results, ATL-1 significantly decreased the percentage of in vitro viability, in a dose-dependent manner. In addition, DAPI staining and flow cytometry tests demonstrated the induction of apoptosis by ATL-I. Western blot analysis indicated that the protein levels of caspase-3, caspase-9 and Bax were increased in A549 and HCC827 cells after ATL-I exposure; to the contrary, the expressions of Bcl-2, Bcl-XL were decreased after treatment with ATL-1. In the in vivo study, ATL-I effectively suppressed tumor growth (A549) in transplanted tumor nude mice with up-regulation of caspase-3, caspase-9, and Bax and down-regulation of Bcl-2 and Bcl-XL. In conclusion, our results demonstrated that ATL-I has significant antitumor activity in lung carcinoma cells, and the possible mechanism of action may be related to apoptosis induced by ATL-I via a mitochondria-mediated apoptosis pathway.
Keywords: atractylenolide I; Atractylodes macrocephala; antitumor activity; apoptosis; lung cancer
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MDPI and ACS Style
Liu, H.; Zhu, Y.; Zhang, T.; Zhao, Z.; Zhao, Y.; Cheng, P.; Li, H.; Gao, H.; Su, X. Anti-Tumor Effects of Atractylenolide I Isolated from Atractylodes macrocephala in Human Lung Carcinoma Cell Lines. Molecules 2013, 18, 13357-13368.
Liu H, Zhu Y, Zhang T, Zhao Z, Zhao Y, Cheng P, Li H, Gao H, Su X. Anti-Tumor Effects of Atractylenolide I Isolated from Atractylodes macrocephala in Human Lung Carcinoma Cell Lines. Molecules. 2013; 18(11):13357-13368.
Liu, Huanyi; Zhu, Yajie; Zhang, Tao; Zhao, Zhenguo; Zhao, Yu; Cheng, Peng; Li, Hua; Gao, Hui; Su, Xiaomei. 2013. "Anti-Tumor Effects of Atractylenolide I Isolated from Atractylodes macrocephala in Human Lung Carcinoma Cell Lines." Molecules 18, no. 11: 13357-13368.