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Molecules 2013, 18(10), 13020-13026; doi:10.3390/molecules181013020
Article

Selection of a Mimotope Peptide of S-adenosyl-l-homocysteine and Its Application in Immunoassays

1 and 2,*
Received: 15 August 2013; in revised form: 9 October 2013 / Accepted: 11 October 2013 / Published: 18 October 2013
(This article belongs to the Section Organic Synthesis)
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Abstract: A competitive immunoassay for S-adenosyl-l-homocysteine (SAH) has been used in the clinical test for homocysteine via an enzymatic conversion reaction. Since S-adenosyl-l-homocysteine is a relatively unstable compound, we have used peptide library phage display to select a new mimotope peptide that interacts with the anti-SAH antibody. By immobilizing the synthetic peptide on solid phase as a competitive surrogate for SAH, we demonstrate its utility in a competitive ELISA assay. The linear range of the assay for SAH was 0.4–6.4 µM, in good correlation to the conventional assay using an SAH-conjugated plate. Our results show that the mimotope peptide has potential to substitute for SAH in immunoassays.
Keywords: homocysteine; competitive assay; phage display; monoclonal antibody; clinical diagnostics homocysteine; competitive assay; phage display; monoclonal antibody; clinical diagnostics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wu, C.; Tzertzinis, G. Selection of a Mimotope Peptide of S-adenosyl-l-homocysteine and Its Application in Immunoassays. Molecules 2013, 18, 13020-13026.

AMA Style

Wu C, Tzertzinis G. Selection of a Mimotope Peptide of S-adenosyl-l-homocysteine and Its Application in Immunoassays. Molecules. 2013; 18(10):13020-13026.

Chicago/Turabian Style

Wu, Chun; Tzertzinis, George. 2013. "Selection of a Mimotope Peptide of S-adenosyl-l-homocysteine and Its Application in Immunoassays." Molecules 18, no. 10: 13020-13026.


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