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Molecules 2013, 18(10), 13020-13026; doi:10.3390/molecules181013020

Selection of a Mimotope Peptide of S-adenosyl-l-homocysteine and Its Application in Immunoassays

1
Health Research Institute, AIST, 1-8-31 Midorigaoka, Ikeda 563-8577, Osaka, Japan
2
New England Biolabs, 240 County Road, Ipswich, MA 01938, USA
*
Author to whom correspondence should be addressed.
Received: 15 August 2013 / Revised: 9 October 2013 / Accepted: 11 October 2013 / Published: 18 October 2013
(This article belongs to the Section Molecular Diversity)
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Abstract

A competitive immunoassay for S-adenosyl-l-homocysteine (SAH) has been used in the clinical test for homocysteine via an enzymatic conversion reaction. Since S-adenosyl-l-homocysteine is a relatively unstable compound, we have used peptide library phage display to select a new mimotope peptide that interacts with the anti-SAH antibody. By immobilizing the synthetic peptide on solid phase as a competitive surrogate for SAH, we demonstrate its utility in a competitive ELISA assay. The linear range of the assay for SAH was 0.4–6.4 µM, in good correlation to the conventional assay using an SAH-conjugated plate. Our results show that the mimotope peptide has potential to substitute for SAH in immunoassays.
Keywords: homocysteine; competitive assay; phage display; monoclonal antibody; clinical diagnostics homocysteine; competitive assay; phage display; monoclonal antibody; clinical diagnostics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Wu, C.; Tzertzinis, G. Selection of a Mimotope Peptide of S-adenosyl-l-homocysteine and Its Application in Immunoassays. Molecules 2013, 18, 13020-13026.

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