Molecules 2013, 18(1), 408-417; doi:10.3390/molecules18010408
Article

Response Surface Methodology Optimization of Fermentation Conditions for Rapid and Efficient Accumulation of Macrolactin A by Marine Bacillus amyloliquefaciens ESB-2

1 Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China 2 Department of Ocean Science and Engineering, Zhejiang University, Hangzhou 310058, China
* Author to whom correspondence should be addressed.
Received: 20 November 2012; in revised form: 5 December 2012 / Accepted: 18 December 2012 / Published: 28 December 2012
(This article belongs to the Section Natural Products)
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Abstract: In the present work, an antibiotic-producing marine bacterium was isolated from a seawater sample collected from Yuhuan, Zhejiang, China, identified and named as Bacillus amyloliquefaciens ESB-2 on the basis of phenotypic characteristics and 16S rRNA gene sequencing. Response surface methodology was applied to optimize the fermentation conditions for rapid and efficient accumulation of macrolactin A, a pharmacologically important marine antibiotic. Eight fermentation conditions were examined for their significance on macrolactin A production using Plackett–Burman factorial design, where peptone, medium volume and temperature significantly improved production rate. Further optimization was carried out using Box-Behnken design of experiments to study the influence of process variables. The optimized fermentation condition for maximum production was peptone 14.8 mg/mL, yeast extract 1 mg/mL, FePO4 0.01 mg/mL, temperature 26.3 °C, initial pH value 6.0, medium volume 72.4%, rotation speed 150 r/min, inoculation 5% and fermented for 2 days. Under the optimized conditions, the concentration of macrolactin A reached 21.63 mg/L, representing a 2.4-fold increase compared to the original standard condition, which was also 17% higher than previous highest report of 18.5 mg/L and three times higher in terms of daily productivity.
Keywords: macrolactin A; fermentation; Bacillus amyloliquefaciens; response surfaces methodology; marine antibiotics

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MDPI and ACS Style

He, S.; Wang, H.; Wu, B.; Zhou, H.; Zhu, P.; Yang, R.; Yan, X. Response Surface Methodology Optimization of Fermentation Conditions for Rapid and Efficient Accumulation of Macrolactin A by Marine Bacillus amyloliquefaciens ESB-2. Molecules 2013, 18, 408-417.

AMA Style

He S, Wang H, Wu B, Zhou H, Zhu P, Yang R, Yan X. Response Surface Methodology Optimization of Fermentation Conditions for Rapid and Efficient Accumulation of Macrolactin A by Marine Bacillus amyloliquefaciens ESB-2. Molecules. 2013; 18(1):408-417.

Chicago/Turabian Style

He, Shan; Wang, Hongqiang; Wu, Bin; Zhou, Hui; Zhu, Peng; Yang, Rui; Yan, Xiaojun. 2013. "Response Surface Methodology Optimization of Fermentation Conditions for Rapid and Efficient Accumulation of Macrolactin A by Marine Bacillus amyloliquefaciens ESB-2." Molecules 18, no. 1: 408-417.

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