Molecules 2013, 18(1), 408-417; doi:10.3390/molecules18010408
Article

Response Surface Methodology Optimization of Fermentation Conditions for Rapid and Efficient Accumulation of Macrolactin A by Marine Bacillus amyloliquefaciens ESB-2

Received: 20 November 2012; in revised form: 5 December 2012 / Accepted: 18 December 2012 / Published: 28 December 2012
(This article belongs to the Section Natural Products)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In the present work, an antibiotic-producing marine bacterium was isolated from a seawater sample collected from Yuhuan, Zhejiang, China, identified and named as Bacillus amyloliquefaciens ESB-2 on the basis of phenotypic characteristics and 16S rRNA gene sequencing. Response surface methodology was applied to optimize the fermentation conditions for rapid and efficient accumulation of macrolactin A, a pharmacologically important marine antibiotic. Eight fermentation conditions were examined for their significance on macrolactin A production using Plackett–Burman factorial design, where peptone, medium volume and temperature significantly improved production rate. Further optimization was carried out using Box-Behnken design of experiments to study the influence of process variables. The optimized fermentation condition for maximum production was peptone 14.8 mg/mL, yeast extract 1 mg/mL, FePO4 0.01 mg/mL, temperature 26.3 °C, initial pH value 6.0, medium volume 72.4%, rotation speed 150 r/min, inoculation 5% and fermented for 2 days. Under the optimized conditions, the concentration of macrolactin A reached 21.63 mg/L, representing a 2.4-fold increase compared to the original standard condition, which was also 17% higher than previous highest report of 18.5 mg/L and three times higher in terms of daily productivity.
Keywords: macrolactin A; fermentation; Bacillus amyloliquefaciens; response surfaces methodology; marine antibiotics
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MDPI and ACS Style

He, S.; Wang, H.; Wu, B.; Zhou, H.; Zhu, P.; Yang, R.; Yan, X. Response Surface Methodology Optimization of Fermentation Conditions for Rapid and Efficient Accumulation of Macrolactin A by Marine Bacillus amyloliquefaciens ESB-2. Molecules 2013, 18, 408-417.

AMA Style

He S, Wang H, Wu B, Zhou H, Zhu P, Yang R, Yan X. Response Surface Methodology Optimization of Fermentation Conditions for Rapid and Efficient Accumulation of Macrolactin A by Marine Bacillus amyloliquefaciens ESB-2. Molecules. 2013; 18(1):408-417.

Chicago/Turabian Style

He, Shan; Wang, Hongqiang; Wu, Bin; Zhou, Hui; Zhu, Peng; Yang, Rui; Yan, Xiaojun. 2013. "Response Surface Methodology Optimization of Fermentation Conditions for Rapid and Efficient Accumulation of Macrolactin A by Marine Bacillus amyloliquefaciens ESB-2." Molecules 18, no. 1: 408-417.

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