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Sulfation and Enhanced Antioxidant Capacity of an Exopolysaccharide Produced by the Medicinal Fungus Cordyceps sinensis
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China
Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
Physical Processing of Agricultural Products Key Lab of Jiangsu Province, Zhenjiang, 212013, Jiangsu, China
* Author to whom correspondence should be addressed.
Received: 22 November 2012; in revised form: 10 December 2012 / Accepted: 19 December 2012 / Published: 24 December 2012
Abstract: EPS-1 was an exopolysaccharide produced by the medicinal fungus Cordyceps sinensis (Cs-HK1). In the present study, EPS-1 was sulfated with chlorosulfonic acid (CSA)-pyridine (Pyr) at different volume ratios, yielding four sulfated derivatives, SEPS-1A, B, C and D, with different degrees of substitution (DS: 0.25–1.38) and molecular weights (17.1–4.1 kDa). The sulfation of EPS-1 occurred most frequently at the C-6 hydroxyl groups due to their higher reactivity. In aqueous solution, the native EPS-1 formed random coils or aggregated networks, but the sulfated derivatives formed single helices. The antioxidant activities of the sulfated EPS-1 derivatives for scavenging hydroxyl radicals (•OH) and 2,2-azinobis-3-ehtylbenzothiazolin-6-sulfonic acid radicals (ABTS•+) were significantly increased with increasing DS and decreasing molecular weight (MW). Sulfation has thus been shown to be an effective and favorable strategy for improving the physico-chemical properties and bioactivities of fungal polysaccharides.
Keywords: Cordyceps sinensis; exopolysaccharide; sulfation; molecular weight; antioxidant activity
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Yan, J.-K.; Wang, W.-Q.; Ma, H.-L.; Wu, J.-Y. Sulfation and Enhanced Antioxidant Capacity of an Exopolysaccharide Produced by the Medicinal Fungus Cordyceps sinensis. Molecules 2013, 18, 167-177.
Yan J-K, Wang W-Q, Ma H-L, Wu J-Y. Sulfation and Enhanced Antioxidant Capacity of an Exopolysaccharide Produced by the Medicinal Fungus Cordyceps sinensis. Molecules. 2013; 18(1):167-177.
Yan, Jing-Kun; Wang, Wen-Qiang; Ma, Hai-Le; Wu, Jian-Yong. 2013. "Sulfation and Enhanced Antioxidant Capacity of an Exopolysaccharide Produced by the Medicinal Fungus Cordyceps sinensis." Molecules 18, no. 1: 167-177.