Molecules 2013, 18(1), 167-177; doi:10.3390/molecules18010167
Article

Sulfation and Enhanced Antioxidant Capacity of an Exopolysaccharide Produced by the Medicinal Fungus Cordyceps sinensis

1email, 2email, 1,3email and 2,* email
Received: 22 November 2012; in revised form: 10 December 2012 / Accepted: 19 December 2012 / Published: 24 December 2012
(This article belongs to the Special Issue Antioxidants 2012)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: EPS-1 was an exopolysaccharide produced by the medicinal fungus Cordyceps sinensis (Cs-HK1). In the present study, EPS-1 was sulfated with chlorosulfonic acid (CSA)-pyridine (Pyr) at different volume ratios, yielding four sulfated derivatives, SEPS-1A, B, C and D, with different degrees of substitution (DS: 0.25–1.38) and molecular weights (17.1–4.1 kDa). The sulfation of EPS-1 occurred most frequently at the C-6 hydroxyl groups due to their higher reactivity. In aqueous solution, the native EPS-1 formed random coils or aggregated networks, but the sulfated derivatives formed single helices. The antioxidant activities of the sulfated EPS-1 derivatives for scavenging hydroxyl radicals (•OH) and 2,2-azinobis-3-ehtylbenzothiazolin-6-sulfonic acid radicals (ABTS•+) were significantly increased with increasing DS and decreasing molecular weight (MW). Sulfation has thus been shown to be an effective and favorable strategy for improving the physico-chemical properties and bioactivities of fungal polysaccharides.
Keywords: Cordyceps sinensis; exopolysaccharide; sulfation; molecular weight; antioxidant activity
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MDPI and ACS Style

Yan, J.-K.; Wang, W.-Q.; Ma, H.-L.; Wu, J.-Y. Sulfation and Enhanced Antioxidant Capacity of an Exopolysaccharide Produced by the Medicinal Fungus Cordyceps sinensis. Molecules 2013, 18, 167-177.

AMA Style

Yan J-K, Wang W-Q, Ma H-L, Wu J-Y. Sulfation and Enhanced Antioxidant Capacity of an Exopolysaccharide Produced by the Medicinal Fungus Cordyceps sinensis. Molecules. 2013; 18(1):167-177.

Chicago/Turabian Style

Yan, Jing-Kun; Wang, Wen-Qiang; Ma, Hai-Le; Wu, Jian-Yong. 2013. "Sulfation and Enhanced Antioxidant Capacity of an Exopolysaccharide Produced by the Medicinal Fungus Cordyceps sinensis." Molecules 18, no. 1: 167-177.

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