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Molecules 2013, 18(1), 167-177; https://doi.org/10.3390/molecules18010167

Sulfation and Enhanced Antioxidant Capacity of an Exopolysaccharide Produced by the Medicinal Fungus Cordyceps sinensis

1
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China
2
Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
3
Physical Processing of Agricultural Products Key Lab of Jiangsu Province, Zhenjiang, 212013, Jiangsu, China
*
Author to whom correspondence should be addressed.
Received: 22 November 2012 / Revised: 10 December 2012 / Accepted: 19 December 2012 / Published: 24 December 2012
(This article belongs to the Special Issue Antioxidants 2012)
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Abstract

EPS-1 was an exopolysaccharide produced by the medicinal fungus Cordyceps sinensis (Cs-HK1). In the present study, EPS-1 was sulfated with chlorosulfonic acid (CSA)-pyridine (Pyr) at different volume ratios, yielding four sulfated derivatives, SEPS-1A, B, C and D, with different degrees of substitution (DS: 0.25–1.38) and molecular weights (17.1–4.1 kDa). The sulfation of EPS-1 occurred most frequently at the C-6 hydroxyl groups due to their higher reactivity. In aqueous solution, the native EPS-1 formed random coils or aggregated networks, but the sulfated derivatives formed single helices. The antioxidant activities of the sulfated EPS-1 derivatives for scavenging hydroxyl radicals (•OH) and 2,2-azinobis-3-ehtylbenzothiazolin-6-sulfonic acid radicals (ABTS•+) were significantly increased with increasing DS and decreasing molecular weight (MW). Sulfation has thus been shown to be an effective and favorable strategy for improving the physico-chemical properties and bioactivities of fungal polysaccharides.
Keywords: Cordyceps sinensis; exopolysaccharide; sulfation; molecular weight; antioxidant activity Cordyceps sinensis; exopolysaccharide; sulfation; molecular weight; antioxidant activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Yan, J.-K.; Wang, W.-Q.; Ma, H.-L.; Wu, J.-Y. Sulfation and Enhanced Antioxidant Capacity of an Exopolysaccharide Produced by the Medicinal Fungus Cordyceps sinensis. Molecules 2013, 18, 167-177.

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