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Molecules 2012, 17(9), 11229-11241; doi:10.3390/molecules170911229
Article

Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from Gymnosperma glutinosum Leaves

1,* , 1, 2, 3, 1, 2, 2, 2 and 2
Received: 16 July 2012; in revised form: 7 September 2012 / Accepted: 10 September 2012 / Published: 20 September 2012
(This article belongs to the Section Metabolites)
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Abstract: Bioassay-guided fractionation of hexane extracts of Gymnosperma glutinosum (Asteraceae) leaves, collected in North Mexico, afforded the known compounds hentriacontane (1) and (+)-13S,14R,15-trihydroxy-ent-labd-7-ene (2), as well as the new ent-labdane diterpene (−)-13S,14R,15-trihydroxy-7-oxo-ent-labd-8(9)-ene (3). In addition, D-glycero-D-galactoheptitol (4) was isolated from the methanolic extract of this plant. Their structures were established on the basis of high-field 1D- and 2D NMR methods supported by HR-MS data. The cytotoxic activity was determined by using the in vitro L5178Y-R lymphoma murine model. Hentriacontane (1) and the new ent-labdane 3 showed weak cytotoxicity, whereas the ent-labdane 2 showed significant (p < 0.05) and concentration dependent cytotoxicity (up to 78%) against L5178Y-R cells at concentrations ranging from 7.8 to 250 µg/mL.
Keywords: Gymnosperma glutinosum; asteraceae; Mexican medicinal plant; lymphoma cells; cancer; ent-Labdane diterpenes Gymnosperma glutinosum; asteraceae; Mexican medicinal plant; lymphoma cells; cancer; ent-Labdane diterpenes
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Quintanilla-Licea, R.; Morado-Castillo, R.; Gomez-Flores, R.; Laatsch, H.; Verde-Star, M.J.; Hernández-Martínez, H.; Tamez-Guerra, P.; Tamez-Guerra, R.; Rodríguez-Padilla, C. Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from Gymnosperma glutinosum Leaves. Molecules 2012, 17, 11229-11241.

AMA Style

Quintanilla-Licea R, Morado-Castillo R, Gomez-Flores R, Laatsch H, Verde-Star MJ, Hernández-Martínez H, Tamez-Guerra P, Tamez-Guerra R, Rodríguez-Padilla C. Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from Gymnosperma glutinosum Leaves. Molecules. 2012; 17(9):11229-11241.

Chicago/Turabian Style

Quintanilla-Licea, Ramiro; Morado-Castillo, Rolando; Gomez-Flores, Ricardo; Laatsch, Hartmut; Verde-Star, María Julia; Hernández-Martínez, Humberto; Tamez-Guerra, Patricia; Tamez-Guerra, Reyes; Rodríguez-Padilla, Cristina. 2012. "Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from Gymnosperma glutinosum Leaves." Molecules 17, no. 9: 11229-11241.



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