Next Article in Journal
A Phytoanticipin Derivative, Sodium Houttuyfonate, Induces in Vitro Synergistic Effects with Levofloxacin against Biofilm Formation by Pseudomonas aeruginosa
Previous Article in Journal
Salvianolic Acid A Protects the Peripheral Nerve Function in Diabetic Rats through Regulation of the AMPK-PGC1α-Sirt3 Axis
Molecules 2012, 17(9), 11229-11241; doi:10.3390/molecules170911229
Article

Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from Gymnosperma glutinosum Leaves

1,* , 1, 2, 3, 1, 2, 2, 2 and 2
1 Departamento de Química, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Universidad S/N, Cd. Universitaria, San Nicolás de los Garza 66451, Nuevo León, Mexico 2 Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Universidad S/N, Cd. Universitaria, San Nicolás de los Garza 66451, Nuevo León, Mexico 3 Institute of Organic and Biomolecular Chemistry, Georg-August-University of Göttingen, Tammannstrasse 2, Göttingen D-37077, Germany
* Author to whom correspondence should be addressed.
Received: 16 July 2012 / Revised: 7 September 2012 / Accepted: 10 September 2012 / Published: 20 September 2012
(This article belongs to the Section Metabolites)
View Full-Text   |   Download PDF [285 KB, uploaded 18 June 2014]   |   Browse Figures

Abstract

Bioassay-guided fractionation of hexane extracts of Gymnosperma glutinosum (Asteraceae) leaves, collected in North Mexico, afforded the known compounds hentriacontane (1) and (+)-13S,14R,15-trihydroxy-ent-labd-7-ene (2), as well as the new ent-labdane diterpene (−)-13S,14R,15-trihydroxy-7-oxo-ent-labd-8(9)-ene (3). In addition, D-glycero-D-galactoheptitol (4) was isolated from the methanolic extract of this plant. Their structures were established on the basis of high-field 1D- and 2D NMR methods supported by HR-MS data. The cytotoxic activity was determined by using the in vitro L5178Y-R lymphoma murine model. Hentriacontane (1) and the new ent-labdane 3 showed weak cytotoxicity, whereas the ent-labdane 2 showed significant (p < 0.05) and concentration dependent cytotoxicity (up to 78%) against L5178Y-R cells at concentrations ranging from 7.8 to 250 µg/mL.
Keywords: Gymnosperma glutinosum; asteraceae; Mexican medicinal plant; lymphoma cells; cancer; ent-Labdane diterpenes Gymnosperma glutinosum; asteraceae; Mexican medicinal plant; lymphoma cells; cancer; ent-Labdane diterpenes
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Quintanilla-Licea, R.; Morado-Castillo, R.; Gomez-Flores, R.; Laatsch, H.; Verde-Star, M.J.; Hernández-Martínez, H.; Tamez-Guerra, P.; Tamez-Guerra, R.; Rodríguez-Padilla, C. Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from Gymnosperma glutinosum Leaves. Molecules 2012, 17, 11229-11241.

View more citation formats

Supplement

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert