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• Li, M
• Zhu, L
• Zhou, C
• Lin, L
• Fan, Y
• Zhuang, Z
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• Zhu, L
• Zhou, C
• Lin, L
• Fan, Y
• Zhuang, Z
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• Zhu, L
• Zhou, C
• Lin, L
• Fan, Y
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Molecules 2012, 17(9), 10716-10723; doi:10.3390/molecules170910716

Article

Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa

Meng Li ^1,2 , Ling Zhu ^2,* , Chun-Ya Zhou ^2,3 , Lin Lin ² , Yan-Jun Fan ²  and Zhi-Meng Zhuang ²

¹ College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China ² Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China ³ College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

* Author to whom correspondence should be addressed.

Received: 4 June 2012; in revised form: 23 August 2012 / Accepted: 24 August 2012 / Published: 7 September 2012

Download PDF Full-Text [186 KB, uploaded 7 September 2012 08:42 CEST]

Abstract: Twenty-five novel EST-derived simple sequence repeat (EST-SSR) markers were developed in the ark shell Scapharca broughtonii. Polymorphisms of these EST-SSR markers were evaluated in 48 wild individuals collected from Shidao, Shandong Province, China. A total of 202 alleles were detected at 25 loci. The numbers of alleles per locus ranged from 4 to 14, with an average of 8.08. The observed and expected heterozygosities varied from 0.2917 to 1.000 and from 0.3570 to 0.9002, respectively. After sequential Bonferroni correction for multiple tests, only one locus was found to deviate from Hardy-Weinberg equilibrium. Twenty-five EST-SSR markers showed a high rate of across-species transferability (100%) in Scapharca subcrenata and a low rate of across-genus transferability (20%) in Tegillarca granosa. These EST-SSRs will be helpful for QTL mapping, molecular breeding and investigation of population genetic diversity in ark shell S. broughtonii and other Scapharca species.

Keywords: ark shell; Scapharca broughtonii; EST-SSR markers; transferability

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