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Molecules 2012, 17(9), 10716-10723; doi:10.3390/molecules170910716
Article

Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa

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Received: 4 June 2012; in revised form: 23 August 2012 / Accepted: 24 August 2012 / Published: 7 September 2012
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Abstract: Twenty-five novel EST-derived simple sequence repeat (EST-SSR) markers were developed in the ark shell Scapharca broughtonii. Polymorphisms of these EST-SSR markers were evaluated in 48 wild individuals collected from Shidao, Shandong Province, China. A total of 202 alleles were detected at 25 loci. The numbers of alleles per locus ranged from 4 to 14, with an average of 8.08. The observed and expected heterozygosities varied from 0.2917 to 1.000 and from 0.3570 to 0.9002, respectively. After sequential Bonferroni correction for multiple tests, only one locus was found to deviate from Hardy-Weinberg equilibrium. Twenty-five EST-SSR markers showed a high rate of across-species transferability (100%) in Scapharca subcrenata and a low rate of across-genus transferability (20%) in Tegillarca granosa. These EST-SSRs will be helpful for QTL mapping, molecular breeding and investigation of population genetic diversity in ark shell S. broughtonii and other Scapharca species.
Keywords: ark shell; Scapharca broughtonii; EST-SSR markers; transferability ark shell; Scapharca broughtonii; EST-SSR markers; transferability
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Li, M.; Zhu, L.; Zhou, C.-Y.; Lin, L.; Fan, Y.-J.; Zhuang, Z.-M. Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa. Molecules 2012, 17, 10716-10723.

AMA Style

Li M, Zhu L, Zhou C-Y, Lin L, Fan Y-J, Zhuang Z-M. Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa. Molecules. 2012; 17(9):10716-10723.

Chicago/Turabian Style

Li, Meng; Zhu, Ling; Zhou, Chun-Ya; Lin, Lin; Fan, Yan-Jun; Zhuang, Zhi-Meng. 2012. "Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa." Molecules 17, no. 9: 10716-10723.



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