Next Article in Journal
Antiproliferative and Molecular Mechanism of Eugenol-Induced Apoptosis in Cancer Cells
Previous Article in Journal
α-Glucosidase Inhibitory Constituents from Acanthopanax senticosus Harm Leaves
Article Menu

Article Versions

Export Article

Open AccessArticle
Molecules 2012, 17(6), 6277-6289; doi:10.3390/molecules17066277

Scutellaria baicalensis Alleviates Cantharidin-Induced Rat Hemorrhagic Cystitis through Inhibition of Cyclooxygenase-2 Overexpression

1
Division of Urology, Department of Surgery, Chi Mei Medical Center, No.21, Taikang, Liuying Dist., Tainan City 73657, Taiwan
2
Graduate Institute of Clinical Medical Research, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan
3
School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan
4
Section of Biologics & Advanced Therapeutic Product Analysis, Division of Research and Analysis, Food and Drug Administration, No.161-2, Kunyang St, Nangang District, Taipei City 11561, Taiwan
5
Graduate Institute of Biomedical Materials, Taipei Medical University, 250 Wu-Hsing Street, Taipei City 11031, Taiwan
*
Author to whom correspondence should be addressed.
Received: 12 April 2012 / Revised: 13 May 2012 / Accepted: 15 May 2012 / Published: 25 May 2012
(This article belongs to the Section Natural Products)
Download PDF [1118 KB, uploaded 18 June 2014]

Abstract

Cantharidin, an active component in mylabris, is used in traditional Chinese medicine (TCM) to treat scabies and hepatoma, but accompanied by hemorrhagic cystitis. Evidence shows that cantharidin induces human bladder carcinoma cell death through COX-2 overexpression in vitro. In TCM, Scutellaria baicalensis is usually used to cure mylabris-induced hematuria. This work was undertaken to determine the mechanisms of cantharidin-induced rat hemorrhagic cystitis and explore the uroprotective effect of S. baicalensis. In vitro results showed cantharidin could induce cytotoxicity through prostaglandin (PG)E2 overproduction of T24 cells. Boiling-water extract of S. baicalensis (SB-WE) could significantly inhibit PGE2 production and COX-2 expression in lipo-polysaccharide-induced RAW 264.7 cells, indicating obvious anti-inflammatory abilities. In vivo results indicated that cantharidin caused rat hemorrhagic cystitis with hematuria via c-Fos and COX-2 overexpression. SB-WE was given orally to cantharidin-treated rats, whereby hematuria level, elevated PGE2 and COX-2 protein overexpression were significantly and dose-dependently inhibited by SB-WE. The anti-inflammatory components of SB-WE are baicalin and wogonin, whose contents were 200.95 ± 2.00 and 31.93 ± 0.26 μg/mg, respectively. In conclusion, cantharidin induces rat cystitis through c-Fos and COX-2 over-expression and S. baicalensis can prevent the resulting hematuria because of its anti-inflammatory effects.
Keywords: mylabris; cantharidin; Scutellaria baicalensis; hemorrhagic cystitis; cyclooxygenase-2; hematuria; c-Fos mylabris; cantharidin; Scutellaria baicalensis; hemorrhagic cystitis; cyclooxygenase-2; hematuria; c-Fos
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Huan, S.-H.; Wang, K.-T.; Yeh, S.-D.; Lee, C.-J.; Lin, L.-C.; Liu, D.-Z.; Wang, C.-C. Scutellaria baicalensis Alleviates Cantharidin-Induced Rat Hemorrhagic Cystitis through Inhibition of Cyclooxygenase-2 Overexpression. Molecules 2012, 17, 6277-6289.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top