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Molecules 2012, 17(5), 5126-5138; doi:10.3390/molecules17055126
Article

Comparison of the Simple Cyclic Voltammetry (CV) and DPPH Assays for the Determination of Antioxidant Capacity of Active Principles

1,* , 1, 1, 1, 2 and 2
1 Department of Chemical Engineering, Physical Chemistry and Organic Chemistry, University of Huelva, Campus de El Carmen, 21071 Huelva, Spain 2 Department of Physical Chemistry and Applied Thermodynamic, Córdoba University, Campus de Excelencia Internacional Agroalimentario (CEIA3), ed. Marie Curie, 14014 Córdoba, Spain
* Author to whom correspondence should be addressed.
Received: 2 March 2012 / Revised: 12 April 2012 / Accepted: 25 April 2012 / Published: 3 May 2012
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Abstract

Antioxidant activity of a number of small (low molecular weight) natural compounds found in spices, condiments or drugs (gallic acid, sesamol, eugenol, thymol, carvacrol, vanillin, salicylaldehyde, limonene, geraniol, 4-hexylresorcinol, etc.) has been evaluated using electrochemical and DPPH radical scavenging measurements. Structural analysis of the tested compound suggest a remarkable activity for phenol derivatives and the importance of the –R groups located on the phenolic ring in the molecule’s ability to act as free radical scavenging as well as their influence in the electrochemical behavior. The voltammetric method can be used for the determination of the antioxidant capability in the same manner as the DPPH radical scavenging because of the correlation found between oxidation potentials and anti-radical power (ARP = 1/EC50). Such electrochemical determination is fast and cheap and allows making measurements under a variety of experimental conditions. The accuracy of the electrochemical measurements is the same for all the compounds, irrespective of their scavenging activity, the opposite of what occurs in the DPPH test.
Keywords: antioxidant activity; cyclic voltammetry; DPPH; phenolics; low weight antioxidants antioxidant activity; cyclic voltammetry; DPPH; phenolics; low weight antioxidants
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Arteaga, J.F.; Ruiz-Montoya, M.; Palma, A.; Alonso-Garrido, G.; Pintado, S.; Rodríguez-Mellado, J.M. Comparison of the Simple Cyclic Voltammetry (CV) and DPPH Assays for the Determination of Antioxidant Capacity of Active Principles. Molecules 2012, 17, 5126-5138.

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