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Molecules 2012, 17(3), 3510-3523; doi:10.3390/molecules17033510
Article

Enrichment and Purification of Deoxyschizandrin and γ-Schizandrin from the Extract of Schisandra chinensis Fruit by Macroporous Resins

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* ,
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 and
*
Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 6 February 2012 / Revised: 7 March 2012 / Accepted: 12 March 2012 / Published: 19 March 2012
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Abstract

In present study, the performance and separation characteristics of 21 macroporous resins for the enrichment and purification of deoxyschizandrin and γ-schizandrin, the two major lignans from Schisandra chinensis extracts, were evaluated. According to our results, HPD5000, which adsorbs by the molecular tiers model, was the best macroporous resin, offering higher adsorption and desorption capacities and higher adsorption speed for deoxyschizandrin and γ-schizandrin than other resins. Columns packed with HPD5000 resin were used to perform dynamic adsorption and desorption tests to optimize the technical parameters of the separation process. The results showed that the best adsorption time is 4 h, the rate of adsorption is 0.85 mL/min (4 BV/h) and the rate of desorption is 0.43 mL/min (2 BV/h). After elution with 90% ethanol, the purity of deoxy-schizandrin increased 12.62-fold from 0.37% to 4.67%, the purity of γ-schizandrin increased 15.8-fold from 0.65% to 10.27%, and the recovery rate was more than 80%.
Keywords: Schisandra chinensis; deoxyschizandrin; γ-schizandrin; macroporous resin; adsorption and desorption; purification Schisandra chinensis; deoxyschizandrin; γ-schizandrin; macroporous resin; adsorption and desorption; purification
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Yang, F.-J.; Ma, C.-H.; Yang, L.; Zhao, C.-J.; Zhang, Y.; Zu, Y.-G. Enrichment and Purification of Deoxyschizandrin and γ-Schizandrin from the Extract of Schisandra chinensis Fruit by Macroporous Resins. Molecules 2012, 17, 3510-3523.

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