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Article

A Convenient Route to 4-Carboxy-4-Anilidopiperidine Esters and Acids

by
János Marton
1,
Brita Glaenzel
1,
Julia Roessler
1,†,
Daniela Golaszewski
1 and
Gjermund Henriksen
2,*,‡
1
ABX Advanced Biochemical Compounds, Biomedizinische Forschungsreagenzien GmbH, Heinrich-Glaeser-Strasse 10-14, D-01454 Radeberg, Germany
2
Department of Nuclear Medicine, Klinikum rechts der Isar, Technische Universität München, Ismaninger Strasse 22, D-81675 Munich, Germany
*
Author to whom correspondence should be addressed.
†
Present Address: Department of Chemistry and Food Chemistry, Organic Chemistry I, Dresden University of Technology, Bergstrasse 66, D-01069 Dresden, Germany.
‡
Present Address: Scintomics Up North AS, Utveien 15, N-1430 As, Norway.
Molecules 2012, 17(3), 2823-2832; https://doi.org/10.3390/molecules17032823
Submission received: 3 January 2012 / Revised: 20 February 2012 / Accepted: 24 February 2012 / Published: 7 March 2012
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Abstract

:
The route selection and development of a convenient synthesis of 4-carboxy-4-anilidopiperidines is described. Previous routes were hampered by the low yield of the target esters as well as the inability to convert the esters to the required free acids. Considerations for large-scale production led to a modified synthesis that utilised a tert-butyl ester of 4-carboxy-4-anilidopiperidines which resulted in a dramatic increase in the overall yield of the target N-propionylated- 4-anilidopiperidine-4-carboxylic acids and their corresponding methyl esters. These compounds are now available for use as precursors and reference standards, of particular value for the production of 11C and 18F-labelled 4-carboxy-4-anilidopiperidine radiotracers.

1. Introduction

The 4-anilidopiperidine (4-AP) [1,2] carfentanil (6a) is a highly potent μ-opioid-receptor (MOR) agonist [3,4]. [11C]6a (Scheme 1) is established for use as a tracer for MOR by means of positron emission tomography (PET) [5,6], whilst 18F-labelled derivatives with a potential for application in this non-invasive imaging technique [6] are in development (e.g., [18F(CH2)2]6k [7,8]). The synthesis of precursors for use in the radiosynthesis of [11C]6a and 18F-labelled analogues of 6a (Scheme 1) relies on compound 4-[N-(1-oxopropyl)-N-phenylamino]-1-(2-phenylethyl)-4-piperidine-carboxylic acid (6g, desmethyl carfentanil free acid), its sodium [9,10,11] or ammonium [12,13] salt [7,8].
Molecules 17 02823 g001 550
Scheme 1. A: Synthesis of 11C-labelled carfentanil and 18F-derivatives. B: Failure to cleave simple alkyl esters of 4-AP-carboxylic acid.
Scheme 1. A: Synthesis of 11C-labelled carfentanil and 18F-derivatives. B: Failure to cleave simple alkyl esters of 4-AP-carboxylic acid.
Molecules 17 02823 g001
These approaches towards tracers for use in PET has so far been by the limited accessibility of acid 6g by cleavage of 6a or its ethyl ester analogue (6j, Scheme 1). Hydrolysis of the carboxylic acid esters in the carfentanil series (6a: R2 = CH3 or 6j: R2 = CH3CH2) with commonly used reagents (e.g., KOH in ethylene glycol), results in the N-despropionyl compound (4a), presumably through an acyl-shift as was observed with the 4-AP sufentanil [14].
The original synthesis of 6a is based on the preparation of α-phenylamino nitrile (2a) from 1-(2-phenylethyl)-4-piperidone (1a), aniline and KCN in a Strecker-addition (route A: 1a→2a→3a→4a→5a→6a, Scheme 2 and Scheme 3). Nitrile hydrolysis yields carboxamide 3a, which is finally reacted with KOH in 1,2-ethanediol at 190 °C to yield the free acid 4a. Conversion to themethyl ester 5a followed by acylation of 5a with propionic anhydride results in 6a. A limitation of this method is the very low overall yield (1.2% [3]) of 6a, mainly caused by low conversion of the nitrile (2a) to the corresponding amide (3a, 14% [4], 3% [13]). Moreover, we found the modified reaction pathway [4] for the synthesis of 6a not to provide any improvement over the original procedure (route B: 1b→2b→3b→4b→5b→6b→6c→6a, 1.2% [15], Scheme 2 and Scheme 3) in contrast to the corresponding yield of 11% in the original report [4]. Furthermore, the preparation of 6g according to procedures reported in the literature (route C: 1a→2a→3a→4a→5c→6i→6g, Scheme 1 and Scheme 2) [4,8,12,13] also resulted in only a very low overall yield (0.5%). Finally, we identified more recently developed methods for the synthesis of 6a, 6g and 6h [13,16] to be applicable only for reactions on the milligram scale.

2. Results and Discussion

The need for gram amounts of pure carfentanil acid for use as precursor in radiolabelling, as well as for the corresponding authentic reference compounds of the radiotracers in question, prompted us to address the development of an improved method of preparing desmethyl carfentanil free acid (6g), desmethyl carfentanil sodium salt (6h) and carfentanil (6a) itself. The literature procedures and the identified improved synthetic sequences are summarized in Scheme 2 and Scheme 3.
Molecules 17 02823 g002 550
Scheme 2. Preparation of 4-phenylamino-1-substituted-4-piperidine carboxylic acid derivatives.
Scheme 2. Preparation of 4-phenylamino-1-substituted-4-piperidine carboxylic acid derivatives.
Molecules 17 02823 g002
For the synthesis of 6a, 6g–h, compound 4b was used as a key intermediate. 4b was prepared [4,15] in a good overall yield (33%). An alternative method [17] for the preparation of 4b was based on the reaction of α-aminonitrile 2b with CSI, followed by cyclization of the resulting amide (7) by treatment with 1 M HCl to yield a 1-phenyl-spirohydantoin (8) derivate. Alkaline hydrolysis [18] of the 2,4-imidazilidinedione derivative yielded α-amino acid 4b in an overall yield of 39%.
For our new synthesis route (route D: 4b→5d→6d→6e→6f→6g→6h and 6g→6a, Scheme 2 and Scheme 3) a tert-butyl group was chosen for protecting the carboxylic acid function of 4b, ultimately providing the new compounds 5d, 6d–f. This protecting group has several advantages: The introduction of tert-butyl goup in 4b is readily performed, and the final cleavage of the tert-butyl ester, subsequent to the required transformations, can be performed under mild conditions.
Molecules 17 02823 g003 550
Scheme 3. Synthesis of 4-carboxy-4-anilidopiperidine derivatives.
Scheme 3. Synthesis of 4-carboxy-4-anilidopiperidine derivatives.
Molecules 17 02823 g003
For the introduction of the tert-butyl group, 4b was reacted with N,N-dimethylformamide di-tert-butyl acetal [19] or, alternatively, with tert-butyl 2,2,2-trichloroacetamidate [20] to yield the tert-butyl ester 5d (71%/43%). N-propionlyation of 5d was initially attempted by refluxing the amine in neat propionic anhydride, but this procedure led to the removal of the tert-butyl group. In contrast propionyl chloride in the presence of Hünig-base yielded 6d in 60% yield. Hydrogenolysis followed by N-alkylation led to the new tert-butylester of carfentanil (6f). Deprotection of 6f with neat TFA at ambient temperature afforded the target compound 6g. Overall yields from 4b were: 6g (16.8%); 6h (13.8%); 6a (13.2%). Overall yields starting from 1b were as follows: 6g (5.54%); 6h (4.55%); 6a 4.35% which compare favourably to that of the literature procedures: 0.4–0.5%.

3. Experimental

3.1. General

Starting materials and reagents were obtained from major commercial suppliers and were used without further purifications. Melting points were measured with a Büchi-535 instrument and the reported data are uncorrected. 1H-NMR and 13C-NMR spectra were obtained with a Bruker 500 spectrometer, and measurements were obtained at 20 °C in CDCl3, CD3OD and DMSO-d6. Column chromatography was performed on silica gel (Kieselgel 60 Merck 1.09385 (0.040–0.063 mm)). TLC was accomplished on Macherey-Nagel Alugram® Sil G/UV254 40 × 80 mm aluminum sheets [0.25 mm silica gel with fluorescent indicator] with the following eluent systems (each (v/v)): [A]: hexane-ethyl acetate 8:2; [B]: chloroform-methanol 9:1; [C]: ethylacetate-methanol 8:2. The spots were visualized with a 254 nm UV lamp or with 5% phosphomolybdic acid in ethanol. Abbreviations: Ph: phenyl; Bn: benzyl; CSI: chlorosulfonyl isocyanate; TBTA: tert butyl 2,2,2-trichloroacetamidate; TFA: trifluoroacetic acid; DIPEA: N-ethyldiisopropylamine (Hünig-base), Caf: carfentanil, tBu: tert butyl group; Bn: benzyl group; βPh: aromatic part of a 2-phenylethyl group.

3.2. Chemistry

tert-Butyl 4-phenylamino-1-benzyl-4-piperidinecarboxylate (5d): Method A: 4-Phenylamino-1-benzyl-4-piperidinecarboxylic acid (4b) (3.1 g, 10 mmol) was dissolved in dry toluene (30 mL) under an argon atmosphere. The mixture was heated to 90 °C and N,N-dimethyformamide di-tert-butyl acetal (9.7 mL, 40 mmol) was added drop-wise over 40 min. The mixture was refluxed (oil-bath 110 °C) for 8h. The product mixture was cooled to ambient temperature and thereafter toluene (30 mL) was added. The organic phase was washed with saturated NaHCO3 solution (2 × 100 mL) and brine (100 mL), dried (Na2SO4) and the solvent was evaporated in vacuo. The crude product was purified by column chromatography (silica gel, eluent: hexane-ethyl acetate 8:2 (v/v). The product was dried in vacuo (3 × 10−1 mbar) for 24 h. Yield: 2.6 g (71%); m.p. 126–127 °C. Rf[A] = 0.18; Rf[B] = 0.90; Rf[C] = 0.88. 1H-NMR (CDCl3) δ = 7.22–7.32 (m, 5H, CH2Ph); 7.12 (t, 2H, NHPh); 6.72 (t, 1H, NHPh); 6.61 (d, 2H, NHPh); 3.78 (s, 1H, NH); 3.51 (s, 2H, CH2Ph); 2.57 (m, 2H, CH2CH2); 2.43 (m, 2H, CH2CH2); 2.21 (m, 2H, CH2CH2); 1.98 (m, 2H, CH2CH2); 1.34 (s, 9H, (CH3)3C). 13C-NMR (CDCl3) δ = 174.3 (COOtBu); 145.5 (NPh-C1); 138.4 (Bn-C1); 129.0 (NPh-C3,5); 128.9 (Bn-C2,6); 128.2 (Bn-C3,5); 127.0 (Bn-C4); 118.3 (NPh-C4); 115.5 (NPh-C2,6); 81.0 ((CH3)3C); 63.0 (PhCH2); 58.8 (C-4); 49.1 (C-2,6); 33.4 (C-3,5); 27.8 ((CH3)3C). HRMS (ESI) Calcd for C23H30N2O2, 366.4966; [M+H]+: 367.2385; Found, [M+H]+: 367.2554.
Method B: To a solution of 4b (4.11 g, 13.24 mmol) in dry dichloromethane (26 mL) and dry tetrahydrofuran (6 mL) tert butyl-2,2,2-trichloroacetamidate (TBTA, 8.71 g, 39.86 mmol) was added under argon atmosphere. Boron trifluoride diethyletherate (0.18 mL, 1.45 mmol) was added carefully at 0 °C and then the reaction mixture was stirred for 72 h at ambient temperature. It was filtered and water (50 mL) was added to the filtrate. The pH of the solution was adjusted to 9 with NH4OH. The suspension was extracted with chloroform (3 × 50 mL). The combined organic phase was dried (Na2SO4), filtered and the solvent was evaporated under reduced pressure. The crude product was purified by column chromatography as above. Yield: 2.13 g (43%).
tert-Butyl 4-[N-(1-oxopropyl)-N-phenylamino]-1-benzyl-4-piperidinecarboxylate (6d): The starting material 5d (3.32 g, 9.05 mmol) was dissolved in dry chloroform (115 mL) and N-ethyldiisopropylamine (7.9 mL, 46 mmol) was added to the solution at ambient temperature. To the resulting mixture, propionyl chloride (2.4 mL, 27.6 mmol) was added drop-wise, and the reaction mixture was refluxed for 8 h. The mixture was cooled to room temperature and poured into water (150 mL). The organic phase was separated and the inorganic phase was extracted with chloroform (3 × 150 mL). The combined organic phases were washed with brine (100 mL), dried (Na2SO4), filtered and evaporated under reduced pressure. The residue was chromatographed on silica gel (eluent 1: hexane-ethyl acetate 7:3 (v/v), eluent 2: hexane-ethyl acetate 1:1 (v/v)). Yield: 2.32 g (60%); yellowish oil; Rf[A] = 0.13; Rf[B] = 0.92; Rf[C] = 0.84. 1H-NMR (CDCl3) δ = 7.18–7.41 (m, 10H, CH2Ph, NPh); 3.47 (s, 2H, CH2Ph); 2.56 (m, 2H, CH2CH2); 2.44 (m, 2H, CH2CH2); 2.25 (m, 2H, CH2CH2); 1.84 (q, J = 7.3 Hz, 2H, COCH2CH3); 1.59 (m, 2H, CH2CH2); 1.50 (s, 9H, (CH3)3C); 0.95 (t, J = 7.3 Hz, 3H, COCH2CH3). 13C-NMR (CDCl3) δ = 173.5 (COOtBu); 172.3 (COCH2CH3); 139.8 (NPh-C1); 138.1 (Bn-C1); 130.6 (NPh-C3,5); 129.1 (Bn-C2,6; Bn-C3,5); 128.4 (Bn-C4); 128.1 (NPh-C2,6); 126.9 (NPh-C4); 80.8 ((CH3)3C); 63.2 (PhCH2); 62.8 (C4); 49.6 (C-2,6); 33.7 (C-3,5); 29.0 (COCH2CH3); 28.0 ((CH3)3C); 9.4 (COCH2CH3). HRMS (ESI) Calcd for C26H34N2O3, 422.5598; [M+H]+: 423.2647; Found, [M+H]+: 423.2767.
tert-Butyl 4-[N-(1-oxopropyl)-N-phenylamino]-4-piperidinecarboxylate (6e): A solution of 6d (4.95 g, 11.7 mmol) in ethanol (200 mL) was hydrogenolysed under heterogenous catalytic conditions (10% Pd/C (1.24 g); 8 bar) at ambient temperature in an autoclave. After 18 h, the catalyst was removed by filtration and the solvent was evaporated. The residue was suspended in water (300 mL) followed by alkalization with NH4OH. The suspension was extracted with chloroform (4 × 150 mL) and the combined organic phase were dried (Na2SO4), filtered, and concentrated in vacuo. The crude material was purified by silica gel column chromatography (eluent: chloroform-methanol 9:1 (v/v)). The product was dried in vacuo (3.5 × 10−1 mbar, 48 h). Yield: 2.87 g (73%); yellowish oil Rf[A] = 0.10; Rf[B] = 0.40; Rf[C] = 0.16. 1H-NMR (CD3OD) δ = 7.35–7.52 (m, 5H, NPh); 2.95 (m, 2H, CH2CH2); 2.80 (m, 2H, CH2CH2); 2.22 (m, 2H, CH2CH2); 1.90 (q, J = 7.5 Hz, 2H, COCH2CH3); 1.53 (s, 9H, (CH3)3C); 1.50 (m, 2H, CH2CH2); 0.95 (t, J = 7.5 Hz, 3H, COCH2CH3). 13C-NMR (CD3OD) δ = 175.9 (COOtBu); 173.4 (COCH2CH3); 140.5 (NPh-C1); 131.6 (NPh-C3,5); 130.6 (NPh-C2,6); 130.1 (NPh-C4); 82.3 ((CH3)3C); 64.6 (C-4); 43.2 (C-2,6); 34.8 (C-3,5); 29.9 (COCH2CH3); 28.3 ((CH3)3C); 9.8 (COCH2CH3). HRMS (ESI) Calcd for C19H28N2O3, 332.4373; [M+H]+: 333.2178; Found, [M+H]+: 333.2322.
tert-Butyl 4-[N-(1-oxopropyl)-N-phenylamino]-1-(2-phenylethyl)-4-piperidinecarboxylate (6f): To a solution of 6e (0.93 g, 2.81 mmol) in N,N-dimethylformamide (15 mL) under an argon atmosphere, triethylamine (0.7 mL, 5 mmol) was added and the mixture stirred for 15 min. 2-Phenylethylbomide (0.5 mL, 3.69 mmol) was added drop-wise to the solution and the reaction mixture was stirred at 70 °C for 24 h. The solvent was evaporated in vacuo. Water (50 mL) and NH4OH (5 mL) was added to the residue and the suspension extracted with chloroform (4 × 50 mL). The combined organic extracts were washed with water (30 mL) and brine (30 mL), dried (Na2SO4), filtered, and concentrated under reduced pressure. The crude product was purified by column chromatography on silica gel (eluent 1: hexane, eluent 2: hexane-ethyl acetate 1:1 (v/v)). The product was dried in vacuo (2 × 10−1 mbar, 12 h). Yield: 0.99 g (81%); colourless oil Rf[A] = 0.18; Rf[B] = 0.80; Rf[C] = 0.81. 1H-NMR (DMSO-d6) δ = 7.21–7.51 (m, 7H, CH2CH2Ph, NPh); 7.14 (m, 3H, NPh); 2.61 (m, 2H, CH2CH2Ph); 2.55 (m, 2H, CH2CH2Ph); 2.39 (m, 2H, CH2CH2); 2.32 (m, 2H, CH2CH2); 2.07 (m, 2H, CH2CH2); 1.76 (q, J = 7.5 Hz, 2H, COCH2CH3); 1.47 (m, 2H, CH2CH2); 1.42 (s, 9H, (CH3)3C); 0.83 (t, J = 7.5 Hz, COCH2CH3). 13C-NMR (DMSO-d6) δ = 172.3 (COOtBu); 171.3 (COCH2CH3); 140.4 (NPh-C1); 139.4 (βPh-C1); 130.4 (NPh-C3,5); 129.3 (βPh-C3,5); 128.5 (βPh-C2,6 and βPh-C4); 128.1 (NPh-C2,6); 125.7 (NPhC4); 79.7 ((CH3)3C); 62.2 (C-4); 59.6 (CH2CH2Ph); 49.1 (C-2,6); 33.0 (CH2CH2Ph); 32.8 (C-3,5); 28.3 (COCH2CH3); 27.6 ((CH3)3C); 9.3 (COCH2CH3). HRMS (ESI) Calcd for C27H36N2O3, 436.5864; [M+H]+: 437.2804; Found, [M+H]+: 437.2887.
4-[N-(1-oxopropyl)-N-phenylamino]-1-(2-phenylethyl)-4-piperidinecarboxylic acid (6g, desmethyl carfentanil free acid, CAS RN: [186022-53-7]): 6f (955 mg, 2.18 mmol) was stirred with TFA (10 mL) at room temperature for 16 h under an atmosphere of argon. The solvent was evaporated and the residue was dried in vacuo (2 × 10−1 mbar, 16 h). The crude product was dissolved in 1M NaOH solution (4 mL) and then water was added (10 mL). The solution was filtered and the pH of the filtrate was adjusted to 5 with 1M HCl solution (3.5 mL) and it was left overnight at 0–4 °C. The precipitate was filtered, washed with cold methanol (10 mL) and dried in vacuo (2 × 10−1 mbar). The free acid was purified by RP-HPLC (Phenomenex 100A, Nucleosil C18; flow: 5 mL/min; mobil phase: methanol-water-acetic acid 70:30:0.1 (v/v/v), λ = 254 nm, tR = 17.6 min). The product was dried in vacuo at room temperature (2 × 10−1 mbar, 16 h). Yield: 563 mg (67%); m.p. 230.0–233.5 °C. Rf[A] = 0.04; Rf[B] = 0.07; Rf[C] = 0.06. 1H-NMR (DMSO-d6) δ = 12.43 (brs, 1H, COOH); 7.19–7.49 (m, 7H, CH2CH2Ph, NPh); 7.14 (m, 3H, NPh); 2.59–2.64 (m, 2H, CH2CH2Ph); 2.52–2.54 (m, 2H, CH2CH2Ph); 2.33–2.43 (m, 4H, 2 × CH2CH2); 2.08 (m, 2H, CH2CH2); 1.75 (q, J = 7.4 Hz, 2H, COCH2CH3); 1.52 (m, 2H, CH2CH2); 0.8 (t, J = 7.4 Hz, COCH2CH3). 13C-NMR (DMSO-d6 + CD3OD) δ = 174.2 (COOH); 173.9 (COCH2CH3); 139.0 (NPh-C1); 137.3 (βPh-C1); 130.9 (NPh-C3,5); 130.4 (βPh-C3,5); 129.9 (βPh-C2,6); 129.5 (βPh-C4); 129.4 (NPh-C4); 127.7 (NPh-C2,6); 60.2 (C-4); 57.2 (CH2CH2Ph); 49.8 (C-2,6); 30.9 (CH2CH2Ph); 30.3 (C-3,5); 29.1 (COCH2CH3); 9.7 (COCH2CH3). C23H28N2O3 (380.48).
4-[N-(1-oxopropyl)-N-phenylamino]-1-(2-phenylethyl)-4-piperidinecarboxylic acid sodium salt (6h, desmethyl carfentanil sodium salt, CAS RN: [98598-82-4]): The free acid 6g (300 mg, 0.78 mmol) was dissolved in dry methanol (120 mL) at 60 °C and the solution was cooled to room temperature. Sodium methylate (43 mg) in dry methanol (10 mL) was given to the above solution and it was stirred at ambient temperature for 30 min. The solvent was removed by rotary evaporation and the residue was dried in vacuo (2 × 10−1 mbar, 72 h). Yield: 260 mg (82%); m.p. 114–116 °C. Rf[A] = 0.06; Rf[B] = 0.11; Rf[C] = 0.10. 1H-NMR (CD3OD) δ = 7.38–7.48 (m, 5H, CH2CH2Ph); 7.23 (m, 2H, NPh); 7.14 (m, 3H, NPh); 2.69–2.75 (m, 4H, CH2CH2Ph and CH2CH2Ph); 2.67 (m, 2H, CH2CH2); 2.51 (m, 2H, CH2CH2); 2.33 (m, 2H, CH2CH2); 1.88 (q, J = 7.4 Hz, 2H, COCH2CH3); 1.07 (m, 2H, CH2CH2); 0.92 (t, J = 7.4 Hz, 3H, COCH2CH3). 13C-NMR (CD3OD) δ = 180.1 (COONa); 175.6 (COCH2CH3); 141.9 (NPh-C1); 141.3 (βPh-C1); 131.9 (NPh-C3,5); 130.1 (βPh-C3,5); 129.6 (βPh-C2,6); 129.4 (NPh-C2,6); 129.4 (βPh-C4); 127.0 (NPh-C4); 66.2 (C-4); 61.5 (CH2CH2Ph); 51.6 (C-2,6); 34.5 (CH2CH2Ph); 34.0 (C-3,5); 30.4 (COCH2CH3); 9.8 (COCH2CH3). C23H27N2NaO3 (402.46).
Methyl 4-[N-(1-oxopropyl)-N-phenylamino]-1-(2-phenylethyl)-4-piperidinecarboxylate (6a, carfentanil free base, CAS RN base: [59708-52-0]; Carfentanil oxalate salt: [61086-44-0]): 6g (1.2 g, 2.87 mmol) was dissolved in dry methanol (20 mL) and refluxed in the presence of cc. sulfuric acid (0.4 mL) for 21 h under argon atmosphere. The solution was taken to room temperature and the solvent removed under reduced pressure. Water (25 mL) was added to the residue, and pH of the mixture adjusted to 9 with NH4OH. The suspension was extracted with a mixture of chloroform-methanol 5:2 (v/v). The organic phase was dried (Na2SO4) and the solvent evaporated. The resulting residue was dissolved in methylisobutylketone (20 mL) and oxalic acid dihydrate (0.38 g, 3 mmol) in methylisobutylketone (15 mL) was added. The white crystalls were filtered and dried in vacuo (3 × 10−1 mbar, 16 h). Yield: 1.1 g (79%); m.p. 183–184 °C. (Lit m.p. 189.5 °C [4], 188–189 °C [21], 182–184 °C [22]) 1H-NMR (DMSO-d6) δ = 7.15–7.50 (m, 10H, CH2CH2Ph, NPh); 3.67 (s, 3H, COOCH3); 3.24 (m, 2H, CH2CH2); 3.05 (m, 2H, CH2CH2Ph); 2.97 (m, 2H, CH2CH2); 2.83 (m, 2H, CH2CH2Ph); 2.23 (m, 2H, CH2CH2); 1.81 (m, 2H, CH2CH2); 1.77 (q, J = 7.4 Hz, 2H, COCH2CH3); 0.79 (t, J = 7.4 Hz, 3H, COCH2CH3). 13C-NMR (CDCl3) δ = 173.2 (COOCH3); 172.4 (COCH2CH3); 164.1 ((COOH)2); 138.4 (NPh-C1); 137.4 (βPh-C1); 130.3 (NPh-C3,5); 129.6 (βPh-C3,5); 129.0 (βPh-C2,6); 128.6 (βPh-C4); 128.5 (NPh-C2,6); 126.6 (NPh-C4); 60.1 (C-4); 56.6 (COOCH3); 52.3 (CH2CH2Ph); 48.6 (C-2,6); 30.3 (CH2CH2Ph); 30.0 (C-3,5); 28.2 (COCH2CH3); 9.0 (COCH2CH3). C24H30N2O3 (394.51). oxalate salt: C26H34N2O7 (484.54).

4. Conclusions

A simple and effective synthesis of 4-carboxy-4-anilidopiperidines has been developed based on coverting 4b to the corresponding t-Bu ester for use as a key intermediate. The improved method facilitates the production of 4-carboxy-4-APs in general, and more specifically, opens a route for preparation of carboxy-4-APs for use in PET imaging.

Supplementary Materials

Supplementary materials can be accessed at: https://www.mdpi.com/1420-3049/17/3/2823/s1.

References and Notes

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  • Sample Availability: Samples of the compounds 3b, 4b, 5d, 6d–g are available from the authors.

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MDPI and ACS Style

Marton, J.; Glaenzel, B.; Roessler, J.; Golaszewski, D.; Henriksen, G. A Convenient Route to 4-Carboxy-4-Anilidopiperidine Esters and Acids. Molecules 2012, 17, 2823-2832. https://doi.org/10.3390/molecules17032823

AMA Style

Marton J, Glaenzel B, Roessler J, Golaszewski D, Henriksen G. A Convenient Route to 4-Carboxy-4-Anilidopiperidine Esters and Acids. Molecules. 2012; 17(3):2823-2832. https://doi.org/10.3390/molecules17032823

Chicago/Turabian Style

Marton, János, Brita Glaenzel, Julia Roessler, Daniela Golaszewski, and Gjermund Henriksen. 2012. "A Convenient Route to 4-Carboxy-4-Anilidopiperidine Esters and Acids" Molecules 17, no. 3: 2823-2832. https://doi.org/10.3390/molecules17032823

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