Molecules 2012, 17(3), 2474-2490; doi:10.3390/molecules17032474
Review

Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads

1, 2, 1,2,* email and 2,3,* email
Received: 22 December 2011; in revised form: 24 February 2012 / Accepted: 24 February 2012 / Published: 1 March 2012
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.
Keywords: optically-encoded bead; fluorescence; quantum dots; surface-enhanced Raman scattering (SERS); bead-based assay; label-free detection; high-throughput screening
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MDPI and ACS Style

Jun, B.-H.; Kang, H.; Lee, Y.-S.; Jeong, D.H. Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads. Molecules 2012, 17, 2474-2490.

AMA Style

Jun B-H, Kang H, Lee Y-S, Jeong DH. Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads. Molecules. 2012; 17(3):2474-2490.

Chicago/Turabian Style

Jun, Bong-Hyun; Kang, Homan; Lee, Yoon-Sik; Jeong, Dae Hong. 2012. "Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads." Molecules 17, no. 3: 2474-2490.

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