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Molecules 2012, 17(3), 2474-2490; doi:10.3390/molecules17032474
Review

Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads

1, 2, 1,2,*  and 2,3,*
1 School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Korea 2 Nano Systems Institute and Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-747, Korea 3 Department of Chemistry Education, Seoul National University, Seoul 151-747, Korea
* Authors to whom correspondence should be addressed.
Received: 22 December 2011 / Revised: 24 February 2012 / Accepted: 24 February 2012 / Published: 1 March 2012
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Abstract

Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.
Keywords: optically-encoded bead; fluorescence; quantum dots; surface-enhanced Raman scattering (SERS); bead-based assay; label-free detection; high-throughput screening optically-encoded bead; fluorescence; quantum dots; surface-enhanced Raman scattering (SERS); bead-based assay; label-free detection; high-throughput screening
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Jun, B.-H.; Kang, H.; Lee, Y.-S.; Jeong, D.H. Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads. Molecules 2012, 17, 2474-2490.

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